Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,568,744 C→A 12.5% V283F (GTC→TTC)  PP_2252 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,568,7440CA12.5% 81.2 / 3.7 32V283F (GTC→TTC) PP_2252hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (13/15);  new base A (2/2);  total (15/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01

AAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTG  >  NC_002947/2568620‑2568889
                                                                                                                            |                                                                                                                                                 
aaGCTGTCCTCAACCTGCCCCGAAAGGTAATGCACCCCCGCCATTGCCCGGCTCGGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATgg                                                                                                                          <  2:184400/150‑1 (MQ=255)
   cTGTCCTCAACCTGCCCCGACAGGTAATAGACGCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAGGTTGAGGATGACCCGCGGTTGTTCGACCAGTTGGGGGTGGTCACGGTCATGGGtt                                                                                                                       >  2:178800/1‑149 (MQ=255)
           aaCCTGCCCCGACAGGTAATAGTCCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCAcccc                                                                                                               <  2:337152/150‑1 (MQ=255)
              cTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTa                                                                                                            >  1:210786/1‑150 (MQ=255)
                          gTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCgg                                                                                                                                          >  2:87628/1‑108 (MQ=39)
                          gTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCgg                                                                                                                                          <  1:87628/108‑1 (MQ=39)
                                   ccccGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGGTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATTGGTCATCACCCCGTAACCGGTTGGGGCAATCGAAat                                                                                       >  1:175682/1‑150 (MQ=255)
                                         cATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGc                                                                                 <  2:334632/150‑1 (MQ=255)
                                               cccGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCgg                                                                                                                                          >  1:108392/1‑87 (MQ=37)
                                               cccGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCgg                                                                                                                                          <  2:108392/87‑1 (MQ=37)
                                                        gccgtcctcCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGcc                                                                  <  1:178800/144‑1 (MQ=255)
                                                        gCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGcc                                                                  >  1:502993/1‑150 (MQ=255)
                                                           gTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCAc                                                               <  2:210786/150‑1 (MQ=255)
                                                            ttCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGCCCATCTTCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACt                                                              <  1:164786/150‑1 (MQ=255)
                                                            ttCTCCATCAACTCCTGCCATTCCTGGATCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGcc                                                                  >  1:255221/1‑146 (MQ=255)
                                                            ttCTCCATCAACTCCTGCCATTCCTGGATCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGcc                                                                  <  2:255221/146‑1 (MQ=255)
                                                                  aTCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGc                                                            <  1:88021/146‑1 (MQ=255)
                                                                  aTCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGc                                                            >  2:88021/1‑146 (MQ=255)
                                                                  aTCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGAt                                                        >  1:292532/1‑150 (MQ=255)
                                                                  aTCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGAt                                                        >  1:252580/1‑150 (MQ=255)
                                                                          cTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACg                                                                                                                                 >  2:235473/1‑69 (MQ=255)
                                                                          cTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACg                                                                                                                                 <  1:235473/69‑1 (MQ=255)
                                                                               aTTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTgg                                           >  2:82895/1‑150 (MQ=255)
                                                                                ttCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGc                                          <  2:252580/150‑1 (MQ=255)
                                                                                      gTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATccc                                    >  1:82008/1‑150 (MQ=255)
                                                                                             gCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTggg                             <  2:502993/150‑1 (MQ=255)
                                                                                                    tCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAggg                      <  1:82895/150‑1 (MQ=255)
                                                                                                        gATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGgc                  >  1:45106/1‑150 (MQ=255)
                                                                                                                gCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCt          <  2:292532/150‑1 (MQ=255)
                                                                                                                        tCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTg  <  2:398959/150‑1 (MQ=255)
                                                                                                                           aCCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCgg                                                                                                 >  1:382701/1‑52 (MQ=255)
                                                                                                                           aCCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCgg                                                                                                 <  2:382701/52‑1 (MQ=255)
                                                                                                                            |                                                                                                                                                 
AAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTG  >  NC_002947/2568620‑2568889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑