| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,861,170 | G→T | 15.4% | Q69K (CAG→AAG) | gllD ← | 4‑oxalomesaconate tautomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,861,170 | 0 | G | T | 15.4% | 64.9 / 3.7 | 26 | Q69K (CAG→AAG) | gllD | 4‑oxalomesaconate tautomerase |
| Reads supporting (aligned to +/- strand): ref base G (11/11); new base T (2/2); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
CGCGCCGCTGGCGGCCACCAGGCCGCGCTCAAGGGCGAACGGCCCTACGCCGGCCAGAATGTTGCCGCAGTTTTGCCCATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTCGGCGCCGCCGATGCCGTCAATCTGCCGGGCATCGGGCGAGCCCATCACCGCCAGCAGCACCCGGTCACGCAGCGGGCCAGGGGCGGGCAGGTCGTCATGCAAAAAAT > NC_002947/2861024‑2861316 | cgcgCCGCTGGCGGCCACCAGGCCGCGCTCAAGGGCGAACGGCCCTACGCCGGCCAGAATGTTGCCGCAGTTTTGCCCATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTTCgc < 2:462581/150‑2 (MQ=255) gcggcCACCAGGCCGCGCTCAAGGGCGAACGGCCCTACGCCGGCCAGAATGTTGCCGCAGTTTTGCCCATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGgatg > 1:131207/1‑150 (MQ=255) gcgcTCAAGGGCGAACGGCCCTACGCCGGCCAGAATGTTGCCGCAGTTTTGCCCATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCt < 1:321466/150‑1 (MQ=255) gcTCAAGGGCGAACGGCCCTACGCCGGCCAGAATGTTGCCGCAGTTTTGCCCATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTgg > 2:71435/1‑150 (MQ=255) aGGGCGAACGGCCCTACGCCGGCCAGAATGTTGCCGCAGTTTTGCCCATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGc < 2:131207/150‑1 (MQ=255) aCGGCCCTACGCCGGCCAGAATGTTGCCGCAGTTTTGCCCATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGc < 1:287369/127‑1 (MQ=255) aCGGCCCTACGCCGGCCAGAATGTTGCCGCAGTTTTGCCCATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGc > 2:287369/1‑127 (MQ=255) cccTACGCCGGCCAGAATGTTGCCGCAGTTTTGCCCATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTCGGcgcc > 1:412562/1‑150 (MQ=255) gCCAGAATGTTGCCGCAGTTTTGCCCATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTCGGCGCCGCCGATGCCg > 1:464067/1‑150 (MQ=255) aGAATGTTGCCGCAGTTTTGCCCATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTCGGCGCCGCCGATGCCGTCa > 2:230629/1‑150 (MQ=255) ttttGCCCATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGa > 1:176837/1‑97 (MQ=255) ttttGCCCATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGa < 2:176837/97‑1 (MQ=255) cATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCTGATGATGGCGACTTTGCTGGTGAGCGAGTc < 2:313174/109‑1 (MQ=255) cATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTc > 1:313174/1‑109 (MQ=255) cATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTCGGCGCCGCCGATGCCGTCAATCTGCCGGGCATCGGGCGAGc < 1:71435/150‑1 (MQ=255) ccTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTCGGCGCCGCCGATGCCGTCAATCTGCCGGGCATCGGGCGAGCCCATCACCGCcagca < 2:308848/150‑1 (MQ=255) tCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTTCGCAGcgcgat > 1:421762/1‑59 (MQ=255) tCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTTCGCAGcgcgat < 2:421762/61‑3 (MQ=38) caccaccacCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTCGGCGCCGCCGATGCCGTCAATCTGCCGGGCATCGGGCGAGCCCATCACCGCCAGCAGCACCCg > 2:464775/1‑150 (MQ=255) ccaccaccTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTCGGCGCCGCCGATGCCGTCAATCTGCCGGGCATCGGGCGAGCCCATCACCGCCAGCAGCACCCGGt > 1:51729/1‑150 (MQ=255) gCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTCGGCGCCGCCGATGCCGTCAATCTGCCGGGCATCGGGCGAGCCCATCACCGCCAGCAGCACCCGGTCACGCAGCggg < 1:349833/150‑1 (MQ=255) cGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTCGGCGCCGCCGATGCCGTCAATCTGCCGGGCATCGGGCGAGCCCATCACCGCCAGCAGCACCCGGTCACGCAGCGGGc < 1:464775/150‑1 (MQ=255) cACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTCGGCGCCGCCGATGCCGTCAATCTGCCGGGCATCGGGCGAGCCCATCACCGCCAGCAGCACCCGGTCACGCAGCGGGCCAGGGGCGGGCAg < 1:230629/150‑1 (MQ=255) tcatccgcgCTTCGCAGCGC‑GATGATGGCGACTTTGCTGGTGAGCGAGTCGGCGCCGCCGATGCCGTCAATCTGCCGGGCATCGGGCGAGCCCATCACCGCCAGCAGCACCCGGTCACGCAGCGGGCCAGGGGCGGGCAGGTCGTCATGc > 1:280031/6‑150 (MQ=255) tCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTCGGCGCCGCCGATGCCGTCAATCTGCCGGGCATCGGGCGAGCCCATCACCGCCAGCAGCACCCGGTCACGCAGCGGGCCAGGGGCGGGCAGGTCGTCATGCaaa > 2:317803/1‑150 (MQ=255) gcTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTCGGCGCCGCCGATGCCGTCAATCTGCCGGGCATCGGGCGAGCCCATCACCGCCAGCAGCACCCGGTCACGCAGCGGGCCAGGGGCGGGCAGGTCGTCATGCAAAAAAt < 2:464067/150‑1 (MQ=255) | CGCGCCGCTGGCGGCCACCAGGCCGCGCTCAAGGGCGAACGGCCCTACGCCGGCCAGAATGTTGCCGCAGTTTTGCCCATAGTCCACCCGCGCCTCGTCCACCACCACCTGAGCGAACAGGTAGTCCACGTCGGCGTCATCGCGCTGCGAAGCGCGGATGATGGCGACTTTGCTGGTGAGCGAGTCGGCGCCGCCGATGCCGTCAATCTGCCGGGCATCGGGCGAGCCCATCACCGCCAGCAGCACCCGGTCACGCAGCGGGCCAGGGGCGGGCAGGTCGTCATGCAAAAAAT > NC_002947/2861024‑2861316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |