breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsPputida_HGL_95_S220_L002_R1_001.good.fq509,36968,085,953100.0%133.7 bases150 bases94.4%
errorsPputida_HGL_95_S220_L002_R2_001.good.fq509,36968,085,953100.0%133.7 bases150 bases92.3%
total1,018,738136,171,906100.0%133.7 bases150 bases93.3%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0029476,181,92920.61.9100.0%Pseudomonas putida KT2440 chromosome, complete genome.
total6,181,929100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000018260
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500097
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.005

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0029470.92937

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input12:34:38 22 Aug 201912:34:53 22 Aug 201915 seconds
Read alignment to reference genome12:34:54 22 Aug 201912:37:07 22 Aug 20192 minutes 13 seconds
Preprocessing alignments for candidate junction identification12:37:07 22 Aug 201912:37:19 22 Aug 201912 seconds
Preliminary analysis of coverage distribution12:37:19 22 Aug 201912:37:47 22 Aug 201928 seconds
Identifying junction candidates12:37:47 22 Aug 201912:38:05 22 Aug 201918 seconds
Re-alignment to junction candidates12:38:05 22 Aug 201912:38:29 22 Aug 201924 seconds
Resolving best read alignments12:38:29 22 Aug 201912:38:51 22 Aug 201922 seconds
Creating BAM files12:38:51 22 Aug 201912:39:17 22 Aug 201926 seconds
Tabulating error counts12:39:17 22 Aug 201912:39:26 22 Aug 20199 seconds
Re-calibrating base error rates12:39:26 22 Aug 201912:39:27 22 Aug 20191 second
Examining read alignment evidence12:39:27 22 Aug 201912:43:25 22 Aug 20193 minutes 58 seconds
Polymorphism statistics12:43:25 22 Aug 201912:43:26 22 Aug 20191 second
Output12:43:26 22 Aug 201912:43:53 22 Aug 201927 seconds
Total 9 minutes 14 seconds