Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947195,6340CT56.1% 4.5 / 43.6 42T380T (ACC→ACT) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (10/8);  new base T (13/10);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAG  >  NC_002947/195485‑195778
                                                                                                                                                     |                                                                                                                                                
gTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGAcaccacc                                                                                                                                                  >  1:276452/1‑150 (MQ=255)
                         aCGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGt                                                                                                                         >  1:248236/1‑150 (MQ=17)
                           gTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGAGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCAAGACCGCGGCGTTGACGGCGAGCCCGTCg                                                                                                                       >  2:691793/1‑150 (MQ=11)
                                   caacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGGCCCCGAATCCCACCCCGGCTCAGACCACGATCAACGCCTGCGTCGACACCACCACCGCGACCCTGACGGCGAGCCCTGCGGTACCCGa                                                                                                               >  2:75031/1‑150 (MQ=11)
                                        gTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcg                                                                                                          <  2:276452/150‑1 (MQ=38)
                                            gACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt                                                                                                      >  1:109869/1‑150 (MQ=38)
                                             aCCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGCGCAACTGACCCCGAATCCGA‑CCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGa                                                                                                    <  2:316902/150‑1 (MQ=255)
                                                    gCGTCACCATCGAGAACGCCGCTGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACtt                                                                                              >  2:593044/1‑150 (MQ=255)
                                                        cACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACCCGATCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGACGTCGTGATCACTTacac                                                                                          >  1:193245/1‑150 (MQ=11)
                                                         aCCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACAcc                                                                                         >  1:527700/1‑150 (MQ=255)
                                                          ccATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCg                                                                                        <  1:379048/150‑1 (MQ=17)
                                                            accGAGAACGCCACCGGCGGTCATTTCGGGCACCTGACCCCGAACCCGACCCCGGCTCAGACCACGCTCCACGACTCGGTCGACACCACCACCCCGCCCCAGCCGCCGACCCCGCCGGTCCCCCCCGGCGACGGGCCCACTTACAcccgg                                                                                      >  2:159750/3‑147 (MQ=9)
                                                            aTCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGt                                                                                       <  1:119493/149‑1 (MQ=12)
                                                            aTCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGt                                                                                       >  2:119493/1‑149 (MQ=12)
                                                            aTCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTg                                                                                      >  2:612089/1‑150 (MQ=255)
                                                             tCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGa                                                                                     >  1:234179/1‑150 (MQ=12)
                                                               gagaACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCCCGCGCAACGACTCGGTCGACACCCCCACCGCGACCCTGACGACGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAcc                                                                                   >  2:172703/1‑150 (MQ=14)
                                                                   aCGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa                                                                               <  2:109869/150‑1 (MQ=40)
                                                                               gTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGccc                                                                   <  2:248236/150‑1 (MQ=21)
                                                                               gTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGcc                                                                    <  1:206692/149‑1 (MQ=25)
                                                                               gTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGcc                                                                    >  2:206692/1‑149 (MQ=25)
                                                                                       gAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGcag                                                           >  1:493365/1‑148 (MQ=12)
                                                                                       gAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCg                                                           <  1:507908/150‑1 (MQ=255)
                                                                                             cTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAAcc                                                     >  1:329636/1‑150 (MQ=255)
                                                                                                 ccccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGa                                                 >  1:657414/1‑150 (MQ=255)
                                                                                                  cccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGaa                                                <  2:527700/150‑1 (MQ=255)
                                                                                                   ccGAATCCGCCCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAAc                                               <  1:30867/150‑1 (MQ=255)
                                                                                                   ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGacc                                               >  1:190880/1‑148 (MQ=255)
                                                                                                   ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGc                                                                     >  1:628385/1‑128 (MQ=37)
                                                                                                   ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGc                                                                     <  2:628385/128‑1 (MQ=38)
                                                                                                   ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAAc                                               <  2:657414/150‑1 (MQ=255)
                                                                                                   ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAAc                                               >  2:209190/1‑150 (MQ=255)
                                                                                                   ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAAc                                               >  1:96177/1‑150 (MQ=255)
                                                                                                    cGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcgg                                                                                                         <  2:271684/91‑1 (MQ=25)
                                                                                                    cGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcgg                                                                                                         >  1:271684/1‑91 (MQ=21)
                                                                                                           gACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCaa                                       >  1:530006/1‑150 (MQ=255)
                                                                                                             ccccGGCTCAGACCACGATCAACGACTCGGTCGACACCACCCCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACg                                     <  1:75031/150‑1 (MQ=255)
                                                                                                                   cTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCaaa                               >  1:626700/1‑150 (MQ=255)
                                                                                                                      aGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGa                            >  1:453150/1‑150 (MQ=255)
                                                                                                                          cACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTAc                        <  1:691793/150‑1 (MQ=255)
                                                                                                                              aTCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGtt                    <  1:593044/150‑1 (MQ=255)
                                                                                                                                   cGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGc               <  2:692312/150‑1 (MQ=255)
                                                                                                                                     ccTCGGTCGACACCACTACCGCAACCCTGACGCCGCGCCCGTCGGGCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg             <  1:505531/149‑1 (MQ=14)
                                                                                                                                                accactaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAg  >  2:436984/1‑150 (MQ=255)
                                                                                                                                                  cactaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGCTGAAGCggg            >  1:456524/1‑138 (MQ=255)
                                                                                                                                                  caccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGa                                                                <  2:410221/86‑1 (MQ=39)
                                                                                                                                                  caccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGa                                                                >  1:410221/1‑86 (MQ=39)
                                                                                                                                                     |                                                                                                                                                
GTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAG  >  NC_002947/195485‑195778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑