| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,988,900 | 0 | C | G | 33.3% | 90.6 / 42.3 | 48 | intergenic (+138/‑408) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base C (9/21); new base G (6/9); total (16/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAA > NC_002947/5988760‑5989039 | tGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTc < 1:56043/150‑1 (MQ=255)tGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTc < 1:344811/150‑1 (MQ=255) cgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTT‑GCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTacaa > 2:666639‑M2/45‑150 (MQ=255) cTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTacaaaca > 2:574435/1‑150 (MQ=255) ttGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGGTTACTCCATGCGAGTTCTTGCAGTGGCAGAC‑ACAGCCGAGCGCCCGGGAGCACGGATCGATATGGCGTAGGacaaaaaa > 1:337522/1‑150 (MQ=12) gAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAgg < 1:66539/150‑1 (MQ=255) gggTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCg < 1:373248/150‑1 (MQ=255) gggTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑CAACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACACGGCg < 2:280027/150‑1 (MQ=255) ccTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCgcgcgcgc < 2:592011/150‑1 (MQ=255) ccTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCgcgcgcgc > 1:594404/1‑150 (MQ=255) gtgAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCATGTGCTGGACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTg < 1:18476/150‑1 (MQ=25) tgAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTgg < 1:284323/150‑1 (MQ=255) agaTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGca < 1:641517/150‑1 (MQ=255) gatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGcac > 2:442458‑M2/19‑150 (MQ=255) gggCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGGGCCCCCTGGCGCACGCGTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGGGCCTGGCACagga < 1:188639/150‑1 (MQ=255) gggCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACagga < 1:692297/150‑1 (MQ=255) agcgcCGCCCGCGCGGCGCTGCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTT‑GCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCaggag < 1:117345/149‑1 (MQ=9) gcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACaggagg < 1:574435‑M2/138‑1 (MQ=255) gcgcCGCCCGCGCGGCTCTTAGCATCTAAGCCCTCTCCTACGTTTGTTTCGGGCCAATTATTCCCG‑GAAGCGCTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCaggagg < 1:683528/150‑1 (MQ=9) ccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGAt > 2:254364‑M2/10‑150 (MQ=255) gcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTg < 1:445032‑M2/143‑1 (MQ=255) ccgcGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTT‑GCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGc < 1:243946/150‑1 (MQ=9) cgcgcgGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTT‑GCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGcc < 2:564138/150‑1 (MQ=9) gcgcTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCTTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaac < 1:442458/150‑1 (MQ=255) ttCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac < 1:254364/150‑1 (MQ=255) cgcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGGGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGt > 1:489460/1‑150 (MQ=255) cgcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTT‑GCGCGCGCCCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTa < 2:467179/150‑1 (MQ=9) cgcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTT‑GCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTa < 1:559113/150‑1 (MQ=2) aaaCCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTT‑GCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCgg < 1:320811/150‑1 (MQ=2) aaaCCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTT‑GCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACCTAGGAGCgg > 2:804/1‑150 (MQ=2) aCCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCggg < 2:594404/150‑1 (MQ=255) gcGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTT‑GCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGttt > 2:485110/2‑150 (MQ=2) ccGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGtt > 2:652847/1‑150 (MQ=255) cTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTT‑GCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcc < 1:666639/150‑1 (MQ=2) cTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTT‑GCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCcgc < 1:556935/150‑1 (MQ=2) gtttgtttCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGc > 2:163337/1‑116 (MQ=255) gtttgtttCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGc < 1:163337/116‑1 (MQ=255) gtttgtttCGGGCCAATTATTCCTGCGGGATTT‑GCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAg > 1:600829/1‑150 (MQ=2) tttgtttCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaag < 2:29467‑M2/150‑10 (MQ=255) ttgtttCGGGCCAATTATTCCTGCGGGATTT‑GCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAgcg > 1:1924/1‑150 (MQ=9) tgtttCGGTCCAGTTATTCCTGAGCGACGC‑GCACGCCAGCCCCCTGGAGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTAAACCcgctcctac < 2:337522/150‑7 (MQ=21) tttCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCggg > 1:598238/1‑133 (MQ=255) tttCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCggg < 2:598238/133‑1 (MQ=255) ttCGGGCCAATTATTCCTGCGGGATTT‑GCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGcgccgc > 2:389806/1‑150 (MQ=9) cAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggc < 2:252780‑M2/150‑22 (MQ=255) cTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcga < 1:641007‑M2/150‑31 (MQ=255) cTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcga < 2:544517‑M2/150‑31 (MQ=255) tgGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcccgcgcggcgcttcgcgg > 1:652847‑M2/1‑117 (MQ=255) ctccgccttggggCATGTCTCGATATCGCGTCGTACAAACGAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaa > 1:117130‑M2/6‑102 (MQ=255) gcgcACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGttt < 1:691282/92‑1 (MQ=255) gcgcACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGttt > 2:691282/1‑92 (MQ=255) | TGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGC‑GCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAA > NC_002947/5988760‑5989039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |