Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,165,388	A→G	I8T (ATC→ACC)	hexR ←	DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,165,388	0	A	G	100.0%	81.9 / NA	26	I8T (ATC→ACC)	hexR	DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/15);  total (11/15)

GCAGAAGCGGTTGACGGTCGGTTCGCTGACCTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGATCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTT  >  NC_002947/1165243‑1165516                                                                                                                                                  |                                                                                                                                 gCAGAAGCGGTTGACGGTCGGTTCGCTGACCTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTg                                                                                                                              >  1:296808/1‑150 (MQ=255) gCAGAAGCGGTTGACGGTCGGTTCGCTGACCTTGGACAATTCGGAACAATCACAGTTGCTGAGACGGATGGTGGTTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTg                                                                                                                              >  1:13521/1‑150 (MQ=255)                          cTGACCTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCa                                                                                                                         >  2:699775/1‑130 (MQ=255)                          cTGACCTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCa                                                                                                                         <  1:699775/130‑1 (MQ=255)                           tGACCTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCa                                                                                                    >  1:391663/1‑150 (MQ=255)                               cTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCAcg                                                                                                           <  1:637540/139‑1 (MQ=255)                               cTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCAcg                                                                                                           >  2:637540/1‑139 (MQ=255)                               cTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATggg                                                                                                >  1:28005/1‑150 (MQ=255)                                  ggCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGgtgt                                                                                             <  2:13521/150‑1 (MQ=255)                                  ggCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGgtgt                                                                                             <  2:28005/150‑1 (MQ=255)                                        cTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCt                                                                                                                               >  1:298389/1‑110 (MQ=255)                                        cTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCt                                                                                                                               <  2:298389/110‑1 (MQ=255)                                        cTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCtt                                                                                       <  2:297202/150‑1 (MQ=255)                                          gggCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTgg                                                                                     <  1:499487/150‑1 (MQ=255)                                          gggCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTgg                                                                                     <  1:639884/150‑1 (MQ=255)                                                 cgcagcgATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCGCGGGTTCCAGGGGTGTGTCCTTGGGTCgggg                                                                              >  2:444663/1‑149 (MQ=255)                                                                         tttttgCGGGTTGTGCCGGATGACTTCGACCACTTTACGTACGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCCGGAAGACAGCCGGCTGTGTGAgcagca                                                      <  2:257725/147‑1 (MQ=255)                                                                           gttgCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAgg                                                    <  2:296808/150‑1 (MQ=255)                                                                                        cAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTTCATGGGTGTGTCCTTGGTTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGgt                                       >  1:432392/1‑150 (MQ=255)                                                                                                                   ggCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTa                            >  1:118168/1‑134 (MQ=255)                                                                                                                   ggCCTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTa                            <  2:118168/134‑1 (MQ=255)                                                                                                                     ccTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACg          <  2:19726/150‑1 (MQ=255)                                                                                                                      cTTGTTCAGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGt         <  2:391663/150‑1 (MQ=255)                                                                                                                             aGCTCGTCGAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACtt  <  1:444663/150‑1 (MQ=255)                                                                                                                                tcgtcgAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGa            <  2:17873/137‑1 (MQ=255)                                                                                                                                tcgtcgAGGCGGCCCTGGGTCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGa            >  1:17873/1‑137 (MQ=255)                                                                                                                                                  |                                                                                                                                 GCAGAAGCGGTTGACGGTCGGTTCGCTGACCTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGATCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTT  >  NC_002947/1165243‑1165516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑