Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,851,740	G→T	G262C (GGC→TGC)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,851,740	0	G	T	100.0%	61.8 / NA	20	G262C (GGC→TGC)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/11);  total (9/11)

TTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGG  >  NC_002947/1851596‑1851869                                                                                                                                                 |                                                                                                                                  ttCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCgg                                                                                                                              >  2:596328/1‑150 (MQ=255)  tCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCggg                                                                                                                             >  1:318124/1‑150 (MQ=255)       tACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGaaa                                                                                                                        <  2:33642/150‑1 (MQ=255)                    aCCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGAAGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAACCATATCTATTcaa                                                                                                           >  1:9723/1‑148 (MQ=255)                     ccAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTAt                                                                                                               >  2:149708/1‑145 (MQ=255)                     ccAGTACAAGCAGATCGCCAAGATGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTAt                                                                                                               <  1:149708/145‑1 (MQ=255)                         tACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTATTCGATCTgg                                                                                                      <  1:444907/150‑1 (MQ=255)                                  aTCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATg                                                                                             <  1:596328/150‑1 (MQ=255)                                                   tGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                            <  1:282672/150‑1 (MQ=255)                                                    gAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAg                                                                           <  2:603210/150‑1 (MQ=255)                                                         gcggcgGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGAAGAACGAGTTGCTGGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATACCTATTCGATCTGGCGCAAGATGCAGTGCAACTGCCTGGAGTTCAg                                                                      <  2:9723/150‑1 (MQ=255)                                                                      gAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTac                                                            <  1:634629/147‑1 (MQ=255)                                                                      gAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTac                                                            >  2:634629/1‑147 (MQ=255)                                                                                                ccAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGcc                               >  1:135100/1‑150 (MQ=255)                                                                                                     tGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGaaa                          <  2:135100/150‑1 (MQ=255)                                                                                                          aaCGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgc                                                     >  1:529201/1‑118 (MQ=255)                                                                                                          aaCGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgc                                                     <  2:529201/118‑1 (MQ=255)                                                                                                                    tCGCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCAAGATCTACGACATGCGTTCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTaca           >  1:577570/1‑150 (MQ=255)                                                                                                                      gCCACTGGCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTATTCGTTCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACAcc         >  1:548760/1‑150 (MQ=255)                                                                                                                             gCGTGAAGGCCGACATCAGCTGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTgg  <  2:560311/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                  TTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGG  >  NC_002947/1851596‑1851869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑