Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,851,755	A→G	I267V (ATC→GTC)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,851,755	0	A	G	96.7%	293.3 / ‑1.4	92	I267V (ATC→GTC)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (42/46);  total (44/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTC  >  NC_002947/1851609‑1851899                                                                                                                                                    |                                                                                                                                                 aGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCt                                                                                                                                               <  2:1663731/150‑1 (MQ=255)    ccGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCAGACATCACCGGGCGGGGGA‑AACATGTCTAtt                                                                                                                                            >  2:2235040/1‑150 (MQ=255)           gTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTg                                                                                                                                     >  1:1995972/1‑150 (MQ=255)             aCAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGCGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGgc                                                                                                                                   <  2:1397294/150‑1 (MQ=255)               aaGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGgcgc                                                                                                                                 <  2:1995972/150‑1 (MQ=255)                cgCAGATCGCCAAGATCTTCCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCa                                                                                                                                >  2:2084731/2‑150 (MQ=255)                 gCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGAACGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCaa                                                                                                                               >  1:969968/1‑150 (MQ=255)                    gATCGCCAAGCTGTTGCATGAGCTGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGAt                                                                                                                            >  1:1300783/1‑150 (MQ=255)                        gCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAg                                                                                                                        >  2:1718798/1‑150 (MQ=255)                        gCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTCGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCCAGATGCAg                                                                                                                        >  2:416602/1‑150 (MQ=255)                          cAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAgcg                                                                                                                      >  1:48261/1‑150 (MQ=255)                              cTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCaaa                                                                                                                  <  2:535336/150‑1 (MQ=255)                               tGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAg                                                                                                                 <  2:969968/150‑1 (MQ=255)                                 ttGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTTATGAACCAGCTGCAGAACGAGTTGCTCGCCACGGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGc                                                                                                               <  2:413068/150‑1 (MQ=255)                                   gCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCTATCTGGCGCAAGATGCAGCGCAAAGGCCt                                                                                                             >  1:452039/1‑150 (MQ=255)                                    cATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTg                                                                                                            <  2:48261/150‑1 (MQ=255)                                     aTGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAg                                                                                                                        <  1:549097/137‑1 (MQ=255)                                     aTGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAg                                                                                                                        >  2:549097/1‑137 (MQ=255)                                      tGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCTATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                          <  2:452039/150‑1 (MQ=255)                                       gAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑ACCCTGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAg                                                                                                         <  1:2235040/150‑1 (MQ=255)                                        aGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGt                                                                                                        <  1:1718798/150‑1 (MQ=255)                                         gcggcgGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGtt                                                                                                       >  1:1887203/1‑150 (MQ=255)                                         gcggcgGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGtt                                                                                                       <  2:2143642/150‑1 (MQ=255)                                         gcggcgGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGtt                                                                                                       <  1:1505709/150‑1 (MQ=255)                                               gcTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGACGGCAT‑GCTAGTCGCTCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCa                                                                                                 >  2:1772673/1‑150 (MQ=255)                                                 tGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGa                                                                                               >  2:1299172/1‑150 (MQ=255)                                                     ccGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTa                                                                                           >  2:262199/1‑150 (MQ=255)                                                         gAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTacgac                                                                                       >  2:381351/1‑150 (MQ=255)                                                            cgcTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtg                                                                                    <  1:712279/150‑1 (MQ=255)                                                               ttCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgcgt                                                                                 >  2:803079/1‑150 (MQ=255)                                                               ttCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgcgt                                                                                 >  2:659123/1‑150 (MQ=255)                                                                 catcaGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACgtgcgtgc                                                                               >  2:76024/1‑150 (MQ=255)                                                                   tcaGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgcgt                                                                                 <  2:76566/146‑1 (MQ=255)                                                                   tcaGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgcgt                                                                                 >  1:76566/1‑146 (MQ=255)                                                                       cGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggtgcg                                                                         >  1:1468224/1‑150 (MQ=255)                                                                         aCGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGAGGTGcgcg                                                                       >  1:1785631/1‑150 (MQ=255)                                                                         aCGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGGGCGTTGcgcg                                                                       >  1:1834199/1‑150 (MQ=255)                                                                            tgatgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGc                                                                    <  2:1887203/150‑1 (MQ=255)                                                                            tgatgaACCAGCTGAAGCACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑GGGTGATGGATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGc                                                                    <  1:396025/150‑1 (MQ=255)                                                                             gatgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCACAAAGGCCTGGAGTTCAGCCAGATCTacgac                                                                                       >  1:516770/1‑130 (MQ=255)                                                                             gatgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCACAAAGGCCTGGAGTTCAGCCAGATCTacgac                                                                                       <  2:516770/130‑1 (MQ=255)                                                                                gaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTg                                                                  <  1:38069/148‑1 (MQ=255)                                                                                gaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTg                                                                  >  2:38069/1‑148 (MQ=255)                                                                                gaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGt                                                                <  2:1300783/150‑1 (MQ=255)                                                                                 aaCCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTg                                                               >  1:534486/1‑150 (MQ=255)                                                                                    cAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTgcgc                                                                        <  1:251167/138‑1 (MQ=255)                                                                                    cAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTgcgc                                                                        >  2:251167/1‑138 (MQ=255)                                                                                       cTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGaa                                                         <  2:1304504/150‑1 (MQ=255)                                                                                        tGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGTAGGGCAATGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGaaa                                                        <  2:441239/150‑1 (MQ=255)                                                                                          cAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATc                                                      <  1:2084731/150‑1 (MQ=255)                                                                                            gagcGAGGTGCGCCCGACCGGCGGGAAGGCCCACATCAGCGGCCGGGCGA‑AACATGTCGATTCGATCTGGCGCAAGATGCCGCGCAAAGGCCGGGAGTTCAGCCAGATCTACGACCTTCGTGCGGTGCGCGTGCTGGTGCCGGAAATCcg                                                    <  1:2144598/147‑1 (MQ=255)                                                                                            gAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCa                                                                                                                         >  1:765202/1‑81 (MQ=255)                                                                                            gAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCa                                                                                                                         <  2:765202/81‑1 (MQ=255)                                                                                               cGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGa                                                 <  1:76024/150‑1 (MQ=255)                                                                                                  gTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTg                                              <  1:1299172/150‑1 (MQ=255)                                                                                                     gCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTa                                           <  2:534486/150‑1 (MQ=255)                                                                                                      cTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTac                                          >  2:1675333/1‑150 (MQ=255)                                                                                                         gCCACTGGCGTGAAGGCCGACATCAGCGGACGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATACAGCGCAAAGGCCTGGAGCTCACCCAGATCTACGACGTTCGTGCGGTGCGCGTGCGGGTGCCGTCAATCCGCGACTGCTACGcc                                       >  2:399486/1‑150 (MQ=255)                                                                                                           cACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGc                                                                    >  2:1763461/1‑119 (MQ=255)                                                                                                           cACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGc                                                                    <  1:1763461/119‑1 (MQ=255)                                                                                                           cACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGAGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACcgc                                     >  1:408121/1‑150 (MQ=255)                                                                                                                   tGAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCa                             <  1:381351/150‑1 (MQ=255)                                                                                                                    gAAGGCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCAt                            <  1:1974917/150‑1 (MQ=255)                                                                                                                     aaGGCCGTCATCAGCCGCCGGGCGA‑AACAGGCCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCAGGAGGTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATc                           <  1:617847/150‑1 (MQ=255)                                                                                                                        gCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTgg                                                                                                           <  2:140545/67‑1 (MQ=255)                                                                                                                        gCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTgg                                                                                                           >  1:140545/1‑67 (MQ=255)                                                                                                                        gCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACcgc                                     >  1:106142/1‑137 (MQ=255)                                                                                                                        gCCGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACcgc                                     <  2:106142/137‑1 (MQ=255)                                                                                                                         ccGGCAACAGCGGGCGGGGGA‑AACATGTCTATTCGATCTGGCGGAAGATGCAGGGCCAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGc                       <  2:1834199/150‑1 (MQ=255)                                                                                                                         ccGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGc                       >  1:1731670/1‑150 (MQ=255)                                                                                                                         ccGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGc                       <  1:1232781/150‑1 (MQ=255)                                                                                                                          cGACATCAGTGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGca                      <  2:408121/150‑1 (MQ=255)                                                                                                                          cGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggtgcg                                                                         >  2:1652424/1‑99 (MQ=255)                                                                                                                          cGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggtgcg                                                                         <  1:1652424/99‑1 (MQ=255)                                                                                                                          cGACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTCCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGca                      >  2:1515451/1‑150 (MQ=255)                                                                                                                           gACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCt                                                                                            <  1:250444/79‑1 (MQ=255)                                                                                                                           gACATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCt                                                                                            >  2:250444/1‑79 (MQ=255)                                                                                                                            aCATCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGcaca                    <  1:659123/150‑1 (MQ=255)                                                                                                                              aTCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACAcc                  >  2:1767651/1‑150 (MQ=255)                                                                                                                               tCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCt                 <  1:262199/150‑1 (MQ=255)                                                                                                                               tCAGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCt                 >  1:1487151/1‑150 (MQ=255)                                                                                                                                 aGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTgg                                <  2:1431696/133‑1 (MQ=255)                                                                                                                                 aGCGGCCGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTgg                                >  1:1431696/1‑133 (MQ=255)                                                                                                                                       cGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTggcgg         <  1:2229586/150‑1 (MQ=255)                                                                                                                                       cGGGCGA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTggcgg         >  2:1858542/1‑150 (MQ=255)                                                                                                                                            gA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCatat    >  2:421365/1‑150 (MQ=255)                                                                                                                                            gA‑AACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCatat    >  2:421020/1‑150 (MQ=255)                                                                                                                                               aaCATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGa                                                 <  1:1380773/103‑1 (MQ=255)                                                                                                                                               aaCATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGa                                                 >  2:1380773/1‑103 (MQ=255)                                                                                                                                               aaCATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTc  <  2:1731670/150‑1 (MQ=255)                                                                                                                                               aaCATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTc  <  1:421365/150‑1 (MQ=255)                                                                                                                                               aaCATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTc  <  1:421020/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 AGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGA‑AACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTC  >  NC_002947/1851609‑1851899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑