Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,655,6870CA42.3% 17.1 / 21.3 26intergenic (‑91/+61)epd/tktAD‑erythrose 4‑phosphate dehydrogenase/transketolase A
Reads supporting (aligned to +/- strand):  ref base C (8/7);  new base A (6/5);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CAGCGGCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTT‑CATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGACT  >  NC_002947/5655541‑5655836
                                                                                                                                                        |                                                                                                                                                      
caGCGGCCGATAAGGTCATAACCTTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTT‑CATGCAAc                                                                                                                                                     <  2:299302/150‑1 (MQ=255)
      ccGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAGAGGTTG‑CATGAAAccctgt                                                                                                                                                <  1:443094/150‑5 (MQ=38)
      ccGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTT‑CATGCAACCTCtgt                                                                                                                                               <  2:951004/150‑1 (MQ=255)
               ccATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTT‑CATGCAACCTCTGTGGGAGCggg                                                                                                                                      <  2:984582/150‑1 (MQ=255)
                cATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTT‑CATGCAACCTCTGTGGGAGCGGGc                                                                                                                                     <  2:638763/150‑1 (MQ=255)
                    aCCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTCGCCTTCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTG‑CATGAAACC‑CTGTGGGAGCGGGc                                                                                                                                     <  1:921065/150‑1 (MQ=18)
                                                tGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTT‑CATGCAACCTCTGTGGGAGCGGGCtt                                                                                                                                   >  1:1109340/1‑120 (MQ=255)
                                                tGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTT‑CATGCAACCTCTGTGGGAGCGGGCtt                                                                                                                                   <  2:1109340/120‑1 (MQ=255)
                                                                 aCGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTT‑CATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCtt                                                                                   >  2:1255872/1‑150 (MQ=255)
                                                                               ttGTCGTCAGCATGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTT‑CATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCg                                                                     <  1:1255872/150‑1 (MQ=255)
                                                                                                                         gggCAAGCCCGCTCCCACAG‑GGTTT‑CATGCAACCTCTGTGGGAGCGAGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAActc                               >  2:416199/1‑150 (MQ=255)
                                                                                                                             aaGCCCGCTCCCACAG‑GGTTT‑CATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCg                           >  2:155900/1‑150 (MQ=255)
                                                                                                                               gCCCGCTCCCACAG‑GGTTT‑CATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGAGCCAAAGCTGGGCGGTAGGGGTAAGCGAAACTCTTCGaa                         >  2:997179/1‑150 (MQ=37)
                                                                                                                                cccGCTCCCACAG‑GGTTT‑CATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTaaa                                                                                           >  1:1061517/1‑83 (MQ=255)
                                                                                                                                cccGCTCCCACAG‑GGTTT‑CATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTaaa                                                                                           <  2:1061517/83‑1 (MQ=255)
                                                                                                                                 ccGCTCCCACAG‑GGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACAACGCCCGCAGATCTTTAAATCAGCCTTCAAGCAGCTCTTCGCCAGTACCAAGGATGTTCTCCAGGGTGAAGCAAAACTCTTCGAACa                       >  2:563574/1‑150 (MQ=25)
                                                                                                                                       ccACAGAGGTTG‑CATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTc                            <  1:491305/139‑1 (MQ=37)
                                                                                                                                       ccACAGAGGTTG‑CATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTc                            >  2:491305/1‑139 (MQ=37)
                                                                                                                                       ccACAGAGGTTG‑CATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCg                 >  2:1072901/1‑150 (MQ=38)
                                                                                                                                       ccACAGAGGTTG‑CATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCg                 >  2:414734/1‑150 (MQ=38)
                                                                                                                                       ccACAGAGGTTG‑CATGAAACC‑CTGTGGGAGCGCGCTTGCCCGCGAATGCCACACCGCAAATTCCGCAGAAATTTAAATCAGTCTTCCAGCAGCTCTTCTGCAGTACCGAGGATGTTATCCAGGGTGAAGCCAAACTCTTCGAACAATGcc                  >  1:561642/1‑150 (MQ=9)
                                                                                                                                       ccACAG‑GGTTT‑CATGCAACCTATGTGGGAGCGGGATTGCCTGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATTAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTACAGGGTGAAGCCAAACTCTTCGAACAATGCCg                 >  2:294293/1‑150 (MQ=255)
                                                                                                                                          caG‑GGTTT‑CATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAg              >  1:387143/1‑150 (MQ=255)
                                                                                                                                               gTTG‑CATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGaa               >  1:1029572/1‑144 (MQ=255)
                                                                                                                                                gttgcATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGaa               <  2:1029572/140‑1 (MQ=255)
                                                                                                                                                    cATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATc                                                                                         <  1:110303/66‑1 (MQ=21)
                                                                                                                                                        cAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTTCCGGGTATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGACt  <  1:155900/150‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                      
CAGCGGCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTT‑CATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGACT  >  NC_002947/5655541‑5655836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑