Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,443,379	C→A	A272S (GCC→TCC)	PP_1262 ←	LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,443,379	0	C	A	100.0%	153.0 / NA	49	A272S (GCC→TCC)	PP_1262	LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/19);  total (30/19)

AGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCATCGGCGGT  >  NC_002947/1443236‑1443520                                                                                                                                                |                                                                                                                                              agcagcTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACac                                                                                                                                         <  2:899383/150‑2 (MQ=255)  gcagcTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAgg                                                                                                                                        <  2:336312/150‑1 (MQ=255)      cTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCa                                                                                                                                    >  1:650383/1‑150 (MQ=255)            aGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTcc                                                                                                                               <  2:995057/149‑1 (MQ=255)            aGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTcc                                                                                                                               >  1:995057/1‑149 (MQ=255)                    aTCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGa                                                                                                                      <  2:796399/150‑1 (MQ=255)                            cTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGTAATACAGGTTCAACTCCTGCAGGCGATACTCCgg                                                                                                              <  2:1195327/150‑1 (MQ=255)                             tGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGc                                                                                                             >  1:463932/1‑150 (MQ=255)                              gATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTTCAGGCTATACTCCGGcc                                                                                                            >  2:270778/1‑149 (MQ=255)                               aTGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAg                                                                                                           >  2:1223805/1‑150 (MQ=255)                                   gTTTTCAGGTCTGCCTCATGCGCGGCAAGAATTTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTAcc                                                                                                       <  1:85326/150‑1 (MQ=255)                                    ttttCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTAccc                                                                                                      <  1:127606/150‑1 (MQ=255)                                         aGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTAcc                                                                                                 >  2:1216984/1‑150 (MQ=255)                                           gTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGc                                                                                                                        >  2:192931/1‑125 (MQ=255)                                           gTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGc                                                                                                                        <  1:192931/125‑1 (MQ=255)                                           gTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAg                                                                                               >  1:1217600/1‑150 (MQ=255)                                            tCTGCCTCATGCGCGGCAAGAATCTCTGGCAGAGAGTTGAGCCGGTACTCGACCCAGGTCTTGAGCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAgg                                                                                              >  2:144129/1‑150 (MQ=255)                                                ccTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTAc                                                                                          >  1:527824/1‑150 (MQ=255)                                                        gcgGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGc                                                                                  >  2:9538/1‑150 (MQ=255)                                                          ggCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAg                                                                                >  1:367312/1‑150 (MQ=255)                                                           gCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGATCCAATTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAATTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAgg                                                                               <  1:341054/150‑1 (MQ=255)                                                           gCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTcc                                                                                     <  1:911818/144‑1 (MQ=255)                                                           gCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTcc                                                                                     >  2:911818/1‑144 (MQ=255)                                                            cAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGc                                                                              >  2:786114/1‑150 (MQ=255)                                                             aaGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGcc                                                                             >  2:1014283/1‑150 (MQ=255)                                                                  tctcTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGa                                                                        >  2:338143/1‑150 (MQ=255)                                                                     ctGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAg                                                                     >  2:874580/1‑150 (MQ=255)                                                                                    gcAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAACCAGCGTTCCCGCAGGCCCTCGATAGCCGAATAGATCGgca                                                      >  1:716996/1‑150 (MQ=255)                                                                                        gTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGTTGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCAc                                                  >  2:858922/1‑150 (MQ=255)                                                                                           cTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGcc                                               >  2:530362/1‑150 (MQ=255)                                                                                            tCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCt                                              >  1:139816/1‑150 (MQ=255)                                                                                                 ccAGTTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGcc                                         >  2:540834/1‑150 (MQ=255)                                                                                                   aGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCa                                       <  2:1139473/150‑1 (MQ=255)                                                                                                    ggTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCAt                                      <  2:650383/150‑1 (MQ=255)                                                                                                    ggTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCAt                                      <  1:615717/150‑1 (MQ=255)                                                                                                        ttGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCAGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCa                                  >  1:379047/1‑150 (MQ=255)                                                                                                              ggggcggccATATGCTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTACgg                            >  2:669633/8‑150 (MQ=255)                                                                                                                 gCATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATc                         >  1:485424/1‑150 (MQ=255)                                                                                                                  cATCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCg                        <  1:144129/150‑1 (MQ=255)                                                                                                                   aTCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCt                                                                           >  1:243129/1‑98 (MQ=255)                                                                                                                   aTCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCt                                                                           <  2:243129/98‑1 (MQ=255)                                                                                                                    tCCAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCa                      <  1:9538/150‑1 (MQ=255)                                                                                                                     ccAGATACTGCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAg                     >  1:1230483/1‑150 (MQ=255)                                                                                                                           aCTTCCGCGATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCa               <  2:463932/150‑1 (MQ=255)                                                                                                                           aCTGCCGCGATGGATAGCTGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCa               <  2:313521/150‑1 (MQ=255)                                                                                                                                 gcgATGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCAt         <  2:1217600/150‑1 (MQ=255)                                                                                                                                    aTGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCATcgg      >  2:1047649/1‑150 (MQ=255)                                                                                                                                     tGGATAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCATcggc     >  2:326402/1‑150 (MQ=255)                                                                                                                                        aTAGATGGAATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCATCGGCGgt  >  1:1115277/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                              AGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCAGTCTTCATGGCATCGGCGGT  >  NC_002947/1443236‑1443520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑