Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,851,755	A→G	I267V (ATC→GTC)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,851,755	0	A	G	100.0%	124.7 / NA	39	I267V (ATC→GTC)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/14);  total (25/14)

CCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATA  >  NC_002947/1851611‑1851896                                                                                                                                                 |                                                                                                                                              cccGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTAt                                                                                                                                          >  1:860074/1‑150 (MQ=255)  ccGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTAtt                                                                                                                                         >  1:656204/1‑150 (MQ=255)     aCCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGa                                                                                                                                      >  2:116028/1‑150 (MQ=255)       cAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCAAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTAGCGAGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATc                                                                                                                                    >  1:634864/1‑150 (MQ=255)          tACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTgg                                                                                                                                 >  2:1252101/1‑150 (MQ=255)            cAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGgcg                                                                                                                               >  2:744579/1‑150 (MQ=255)               gCAGATCGCCAAGCTGTTGCATGAGCGGCGGTTGGACCGCGAGTGCTTAATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGGCGATATCAGCGGCCTGGCGAAACATGTCTATTCGATCTGGCGCaa                                                                                                                            <  2:265085/150‑1 (MQ=255)                 aGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGa                                                                                                                          <  2:788212/150‑1 (MQ=255)                 aGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGa                                                                                                                          >  2:1111209/1‑150 (MQ=255)                           gCTGTTGCATGAGCGGCGGCTAGACCGCGAGCGCTTCATCAGCGACGTTATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACAAGTCTATTCGATCTGGCGCAAGATGCAGCGCaa                                                                                                                >  1:1223553/1‑150 (MQ=255)                                    tGAGCGGCGGCTGGACCGCGATCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTAGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                       >  1:698500/1‑150 (MQ=255)                                    tGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                       <  2:732857/150‑1 (MQ=255)                                          gcggcTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAg                                                                                                 >  2:544705/1‑150 (MQ=255)                                                ggACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGAt                                                                                           <  1:313346/150‑1 (MQ=255)                                                        aGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTatgacg                                                                                   >  2:1249361/1‑150 (MQ=255)                                                         tcgcTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGTTCGCCACTGTCGTGAAGTCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTACAGCCAGATCTACGAcgt                                                                                  <  2:10256/149‑1 (MQ=255)                                                           gcTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGAGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgc                                                                                <  1:996016/150‑1 (MQ=255)                                                                  caGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGTGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggt                                                                         <  1:79514/150‑1 (MQ=255)                                                                   aGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggt                                                                         <  1:285471/149‑1 (MQ=255)                                                                   aGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggt                                                                         >  2:285471/1‑149 (MQ=255)                                                                      gACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTgcgc                                                                     >  1:734925/1‑150 (MQ=255)                                                                           gatgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCt                                                                >  1:843647/1‑150 (MQ=255)                                                                            atgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTg                                                               <  2:612119/150‑1 (MQ=255)                                                                            atgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTg                                                               >  1:1150477/1‑150 (MQ=255)                                                                              gaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGt                                                             >  2:493988/1‑150 (MQ=255)                                                                                 ccAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGcc                                                          >  1:500535/1‑150 (MQ=255)                                                                                          gAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTACTCGATCTAGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGGAATCcg                                                 >  1:1057176/1‑150 (MQ=255)                                                                                             cGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGa                                              >  1:511264/1‑150 (MQ=255)                                                                                                     tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTaca                                      >  2:737499/1‑150 (MQ=255)                                                                                                                gTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGcc                                                                                               <  1:1148858/82‑1 (MQ=255)                                                                                                                gTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGcc                                                                                               >  2:1148858/1‑82 (MQ=255)                                                                                                                   aaGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGGTGGGCATc                        <  2:500535/150‑1 (MQ=255)                                                                                                                     ggCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGGGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGt                      <  1:1252101/150‑1 (MQ=255)                                                                                                                       ccGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGc                    >  2:704613/1‑150 (MQ=255)                                                                                                                         gACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGCGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGcac                  >  1:543941/1‑150 (MQ=255)                                                                                                                            aTCAGCGGCCGGGCGAAACATGTCTATTCGCTCTGGCGCAAGATACAGCGCAAAGGCCTGGAATTCAGCCAGATCTACGACGTGCATGCGGTGCGCGTGCTAGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACAcc               >  1:555578/1‑150 (MQ=255)                                                                                                                                         cGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGACAGATCTACGACGTGCGTGCGGTGCGCTTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGAata  <  2:634864/150‑1 (MQ=255)                                                                                                                                             aCATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTg                              >  1:875604/1‑118 (MQ=255)                                                                                                                                             aCATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTg                              <  2:875604/118‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                              CCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATA  >  NC_002947/1851611‑1851896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑