Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,635,606	T→G	T66P (ACC→CCC)	uvrY ←	BarA/UvrY two‑componenent system response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,635,606	0	T	G	100.0%	130.0 / NA	44	T66P (ACC→CCC)	uvrY	BarA/UvrY two‑componenent system response regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/25);  total (19/25)

CCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAA  >  NC_002947/4635462‑4635752                                                                                                                                                 |                                                                                                                                                   ccTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCt                                                                                                                                               >  1:1221641/1‑150 (MQ=255)    ggACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCa                                                                                                                                            <  2:436365/150‑1 (MQ=255)     gACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAg                                                                                                                                           <  1:424090/150‑1 (MQ=255)      aCCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCTgg                                                                                                                                          <  1:1316928/150‑1 (MQ=255)          tttCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGgccgc                                                                                                                                      >  2:697700/1‑150 (MQ=255)              gTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACACGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGAt                                                                                                                                  >  2:904293/1‑150 (MQ=255)                 aaGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACAGTAACCTCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGcc                                                                                                                               >  2:38857/1‑150 (MQ=255)                    gCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCggg                                                                                                                            >  2:786857/1‑150 (MQ=255)                     ccTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGc                                                                                                                           <  2:745324/150‑1 (MQ=255)                       tGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCAt                                                                                                                         >  1:652552/1‑150 (MQ=255)                         cACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCAttt                                                                                                                       >  2:1291445/1‑150 (MQ=255)                          ccccTTGGTCAGGTAGCCGGCAGCGGCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCCCCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCAtttt                                                                                                                      <  2:1174047/149‑1 (MQ=255)                                 gTCAGGTAGCCGGCAGCGCCTGCATGCAACAAGCGTGTGTGGAACGGGTCTTCCTCGCACACCGTCACTGCCACTACCTTGATGTCAGGGTAGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCAGTTTCAcatcc                                                                                                               <  2:404050/150‑1 (MQ=255)                                 gTCAGGTAGCCGGCAGCGCCGGCCTGTGGTAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCAcatcc                                                                                                               >  1:362479/1‑150 (MQ=255)                                    aGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGTCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCAcatccatc                                                                                                            <  1:74284/150‑1 (MQ=255)                                       tAGCCGGCAGCGCCGGCCTGCAACACGCGTGTGGGGAACGGGTCTTCCTCGCACACCGCCACCGCCCCTACCCTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGc                                                                                                         <  2:926448/150‑1 (MQ=255)                                         gCCGGCAGCGCCGGTCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTcc                                                                                                                                             >  1:354871/1‑112 (MQ=255)                                         gCCGGCAGCGCCGGTCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTcc                                                                                                                                             <  2:354871/112‑1 (MQ=255)                                         gCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTAGGTCAGGGTGGGCCGCAGGTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGcac                                                                                                       >  1:674047/1‑150 (MQ=255)                                             gCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGTCGATGCCGGGCGTTTTCACATCCATCAGCACAACg                                                                                                   >  2:1292214/1‑150 (MQ=255)                                             gCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACg                                                                                                   >  2:1213322/1‑150 (MQ=255)                                                gcgcCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAACACAACGTcc                                                                                                >  2:1076567/1‑150 (MQ=255)                                                  gcCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCgg                                                                                              <  2:320170/150‑1 (MQ=255)                                                        cTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAg                                                                                        >  1:855906/1‑150 (MQ=255)                                                                 gCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGc                                                                               <  2:1221641/150‑1 (MQ=255)                                                                    tgtgGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAg                                                                            >  2:1224313/1‑150 (MQ=255)                                                                         ggAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGTCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCa                                                                       <  1:1292214/150‑1 (MQ=255)                                                                          gAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGTCGGGCATTTTTACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAg                                                                      <  1:483754/150‑1 (MQ=255)                                                                          gAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAg                                                                      <  2:738909/150‑1 (MQ=255)                                                                                gTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGa                                                                >  2:884811/1‑150 (MQ=255)                                                                                          cacaCCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGaa                                                      >  2:594162/1‑150 (MQ=255)                                                                                          cacaCCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAACACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGaa                                                      <  1:1076567/150‑1 (MQ=255)                                                                                                gTCACCGCCACTACCTTGATGTCAGGGTGGCTTCGCATCAGTGTGCGTGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTccc                                                >  1:357240/1‑150 (MQ=255)                                                                                                 tCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCt                                               <  2:357240/150‑1 (MQ=255)                                                                                                      gCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTcaccc                                          >  2:1184459/1‑150 (MQ=255)                                                                                                        cACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTcacccac                                        <  1:786857/150‑1 (MQ=255)                                                                                                                tGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCGCCCACCACCTGCa                                <  1:232941/150‑1 (MQ=255)                                                                                                                     tCAGGGTGGCTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCCCCACCTGCAGGCCg                           <  2:652552/150‑1 (MQ=255)                                                                                                                              gtGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCTGGCCAGTTTTAGTGCCGTCTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCg                  <  1:808746/149‑1 (MQ=255)                                                                                                                              cTGCGCAGCAGTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCg                  <  1:192783/150‑1 (MQ=255)                                                                                                                                 cgcAGCATTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGCCTCGCCGGAATCTCCCTCGCCCACCCCCTGCAGGCCGTCGATGTCGGcc               <  2:1147168/150‑1 (MQ=255)                                                                                                                                        gTTTGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTACCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATcc        <  1:697700/150‑1 (MQ=255)                                                                                                                                        gTTTGCGGGGGGCCTCAAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGTGCCAGTTTCAGTGCCGACGCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATcc        <  1:1311627/150‑1 (MQ=255)                                                                                                                                          ttGCGGGGGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCTCCGGAATCTCCCTCTCCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCgt      <  1:38857/150‑1 (MQ=255)                                                                                                                                              ggggggCCTCCAGCCCGCCGATGACGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAAGTTCGCTCACCCACCACCTGCATGCCGTCGATGTCGGCAAACATCCGTGTaa  >  2:142448/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                   CCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAA  >  NC_002947/4635462‑4635752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑