| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,655,692 | Δ1 bp | 41.7% | intergenic (‑96/+56) | epd ← / ← tktA | D‑erythrose 4‑phosphate dehydrogenase/transketolase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,655,692 | 0 | T | . | 41.7% | 38.5 / 34.7 | 24 | intergenic (‑96/+56) | epd/tktA | D‑erythrose 4‑phosphate dehydrogenase/transketolase A |
| Reads supporting (aligned to +/- strand): ref base T (7/6); new base . (6/4); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
CGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGACTCACCG > NC_002947/5655548‑5655841 | cGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtgtg < 2:1178178/150‑1 (MQ=255)cGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtgtg < 1:278697/150‑1 (MQ=255) gACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtgtg < 1:1051829/114‑1 (MQ=255) gACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtgtg > 2:1051829/1‑114 (MQ=255) cAGTTAAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCcagca > 1:181130/1‑150 (MQ=255) ttCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCa < 2:828527/136‑1 (MQ=255) ttCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCa > 1:828527/1‑136 (MQ=255) tcAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGc > 1:1023780/1‑77 (MQ=14) tcAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGc < 2:1023780/77‑1 (MQ=14) cGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCGCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAg < 2:581695/150‑1 (MQ=255) gggCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTtctc > 2:233040/1‑132 (MQ=255) gggCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTtctc < 1:233040/132‑1 (MQ=255) gCCCGCGCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGaa > 2:1204058/1‑150 (MQ=255) cccGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAAT‑‑‑‑GCCACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATg > 1:230206/1‑150 (MQ=21) ccGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAg > 1:194220/1‑115 (MQ=255) ccGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAg < 2:194220/115‑1 (MQ=255) ccACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTc > 2:331435/1‑139 (MQ=37) ccACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTc < 1:331435/139‑1 (MQ=37) cacagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGt > 2:472177/7‑123 (MQ=37) cacagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGt < 1:472177/117‑1 (MQ=37) cacagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAAT‑‑‑‑GCCACACCGCAAATTCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCg > 1:909937/7‑150 (MQ=12) acagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGTGTGAAGCCAAACTCTTc < 1:658778/132‑1 (MQ=37) acagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGTGTGAAGCCAAACTCTTc > 2:658778/6‑137 (MQ=37) gTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCgg > 1:539002/1‑150 (MQ=255) ctctGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTATTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCaaa < 1:268306/114‑1 (MQ=255) ctctGTGGGAGCGGGATTGCCCGCGCCTGAAGGCCAAACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCAAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGACTCAcc > 2:740773/1‑150 (MQ=255) ctctGTGGAAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCATCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCaaa > 2:268306/1‑114 (MQ=255) tctGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGACTCACCg > 2:546327/1‑150 (MQ=255) | CGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGC‑‑‑‑‑CCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGACTCACCG > NC_002947/5655548‑5655841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |