Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,380,728 Δ1 bp 11.8% intergenic (‑110/+25) PP_t67 ← / ← PP_t66 tRNA‑Met/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,380,7280G.11.8% 137.6 / 15.6 51intergenic (‑110/+25)PP_t67/PP_t66tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base G (22/21);  new base . (2/4);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

AGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCA  >  NC_002947/1380601‑1380868
                                                                                                                               |                                                                                                                                              
aGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGaa                                                                                                                        >  2:948669/1‑150 (MQ=255)
 ggCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGCAt                                                                                                                       <  1:948669/150‑1 (MQ=255)
 ggCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAAt                                                                                                                       >  1:168518/1‑150 (MQ=255)
    tgctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTg                                                                                                                    <  2:606079/150‑1 (MQ=255)
       tCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGT‑TAACACAGTGGCC‑tttttcttt                                                                                                                         <  1:610056/142‑6 (MQ=21)
       tCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGT‑TAACACAGTGGCC‑tttttcttt                                                                                                                         >  2:610056/1‑137 (MQ=21)
           tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTGTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCgg                                                                                                             <  1:1121573/150‑1 (MQ=255)
           tCCCGCGTCAGTGGGGCGGATTCTAAAGTGGGCGGTTTGTGTGTCAAGGGCTAGGCTTGGATATTTCGACGTGTTTTCGCGAATGCTAATCAACAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑ATATCTTGAATCTGGTTGCgg                                                                                                             >  2:361526/1‑150 (MQ=18)
              cgcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTgg                                                                                                                                     >  1:351508/1‑124 (MQ=255)
              cgcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTgg                                                                                                                                     <  2:351508/124‑1 (MQ=255)
                    aGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGAt                                                                                                    <  1:315829/150‑1 (MQ=255)
                     gTGGGGAGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGAtt                                                                                                   >  2:547697/1‑150 (MQ=255)
                         ggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGaa                                                                                               >  2:612968/1‑150 (MQ=255)
                          gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAAC‑ACAGTGGCC‑tttttcttt                                                                                                                         >  1:985036/1‑118 (MQ=25)
                          gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGT‑TAACACAGTGGCC‑tttttcttt                                                                                                                         <  2:984976/123‑6 (MQ=25)
                          gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGT‑TAACACAGTGGCC‑tttttcttt                                                                                                                         <  2:985036/123‑6 (MQ=25)
                          gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGT‑TAACACAGTGGCC‑tttttcttt                                                                                                                         >  1:984976/1‑118 (MQ=25)
                          gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGAAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCT‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                              >  1:775895/1‑150 (MQ=255)
                                    cAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAcc                                                                                             >  1:530792/1‑141 (MQ=255)
                                    cAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAcc                                                                                             <  2:530792/141‑1 (MQ=255)
                                       tgtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGc                                                                                                                                    >  2:1142515/1‑100 (MQ=255)
                                       tgtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGc                                                                                                                                    <  1:1142515/100‑1 (MQ=255)
                                       tgtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTATTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                 <  2:1053653/150‑1 (MQ=255)
                                            ggTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                            <  1:860219/150‑1 (MQ=255)
                                            gctttGTATGTCAAGCGCTAGTTTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTTGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                            <  1:495307/148‑1 (MQ=255)
                                                  tATGTCAAGCGCTAGGCTTTGATTTTTTTATTTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                      >  2:477265/1‑150 (MQ=255)
                                                            gcTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTAc                                                            <  1:547697/150‑1 (MQ=255)
                                                             cTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑AAAGTGGCC‑TTTTCTTGAATGTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACAAGCTaac                                                           >  2:305667/1‑148 (MQ=255)
                                                                ggCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCAGGAGCCTGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAgg                                                        <  2:168518/150‑1 (MQ=255)
                                                                   tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCtt                                                                                                                           >  1:4061/1‑80 (MQ=255)
                                                                   tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCtt                                                                                                                           <  2:4061/80‑1 (MQ=255)
                                                                      gATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTc                                                  >  1:1029901/1‑150 (MQ=255)
                                                                                    ttttCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTa                                                             <  2:57381/125‑1 (MQ=255)
                                                                                    ttttCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTa                                                             >  1:57381/1‑125 (MQ=255)
                                                                                      ttCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgg                                  <  2:1029901/150‑1 (MQ=255)
                                                                                        cgcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTGCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgggg                                <  1:361526/150‑1 (MQ=255)
                                                                                               gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTc                         >  2:354770/1‑150 (MQ=255)
                                                                                                     cagcagGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTa                       >  2:1191397/1‑146 (MQ=255)
                                                                                                     cagcagGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTa                       <  1:1191397/146‑1 (MQ=255)
                                                                                                      agcagGCAAAGAAAAACGCCACTGAGCTA‑ACAGTGGCC‑TTTTCTTGCATCGGTTTGCGGGATCCGGATTTGAACCGACGACCTTCGCGTTATGATCCCCACGAGCTACCTGACTTCCCCCCCCCACGGCAGTGGGGCGGATTCTACAggg                  >  2:169982/1‑148 (MQ=14)
                                                                                                           gCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAg             <  1:354770/150‑1 (MQ=255)
                                                                                                           gCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAg             <  1:477265/150‑1 (MQ=255)
                                                                                                             aaaGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGGCAGGGGGGCGGATTCTACAGCGTGTCGtt           >  1:233593/1‑150 (MQ=255)
                                                                                                              aaGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAg                                                                <  2:197952/96‑1 (MQ=39)
                                                                                                              aaGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAg                                                                >  1:197952/1‑96 (MQ=39)
                                                                                                                gAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTtat        >  2:1092840/1‑150 (MQ=255)
                                                                                                                  aacatgCCACTGCGTGA‑ACATTTTCC‑TTTTCGTGAATCTGGTTTCGGGAGCCGGATTTGAACCGACGTCCTTCGGGTTATGAGCCCGACGGGCGACCTGTTTGCTACATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTatat      <  2:233593/145‑1 (MQ=14)
                                                                                                                    aaGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGt    >  1:178827/1‑150 (MQ=255)
                                                                                                                    aaGGCCACTGT‑TAACACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTatat      <  1:191438/148‑1 (MQ=255)
                                                                                                                      ggCCACTGTTAAC‑ACAGTGGCCTTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGGAGTTTATATGTc   >  1:1151557/1‑150 (MQ=17)
                                                                                                                      ggCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCa  >  1:1075665/1‑150 (MQ=255)
                                                                                                                               |                                                                                                                                              
AGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTA‑ACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCA  >  NC_002947/1380601‑1380868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑