| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,218,711 | Δ1 bp | 9.8% | intergenic (+51/+32) | oprJ → / ← nfxB | outer membrane protein OprJ/HTH‑type transcriptional regulator NfxB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,218,711 | 0 | G | . | 9.8% | 157.2 / 12.2 | 51 | intergenic (+51/+32) | oprJ/nfxB | outer membrane protein OprJ/HTH‑type transcriptional regulator NfxB |
| Reads supporting (aligned to +/- strand): ref base G (23/23); new base . (3/2); total (26/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01 | |||||||||||
GAACGAAGCAGCCTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCATCGACGATGCCGTACACCATGCT > NC_002947/3218564‑3218856 | gAACGAAGCAGCCTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGcc < 2:726560/150‑1 (MQ=255) aaGCAGCCTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGGCATCGAT‑CCCTggg < 1:71375/150‑1 (MQ=255) aaGCAGCCTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTggg < 1:1033816/150‑1 (MQ=255) aaGCAGCCTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTggg < 1:537114/150‑1 (MQ=255) ccTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGttttt < 1:954554/150‑1 (MQ=255) cTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGtttttt > 2:534141/1‑150 (MQ=255) tCATCGAAGCCAGACCCCTGCGACACCTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCTGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTg < 2:10860/150‑1 (MQ=255) ccAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCCGCTGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGaa < 1:866232/150‑1 (MQ=255) aCCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAg < 1:534141/150‑1 (MQ=255) ccAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCTAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGTGTTTTTTTGGCTGGTGAAGGCAGGt < 2:362857/150‑1 (MQ=255) ccAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCt < 1:986426/137‑1 (MQ=255) ccAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCt > 2:986426/1‑137 (MQ=255) cAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTc > 1:595110/1‑150 (MQ=255) aGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCa < 1:1025014/150‑1 (MQ=255) gACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCCCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCagcg > 2:1058572/1‑150 (MQ=255) aCAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCagcga > 2:561956/1‑150 (MQ=255) cTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCgg < 2:595110/150‑1 (MQ=255) gCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTg > 2:409400/1‑150 (MQ=255) gTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGcc > 2:577571/1‑150 (MQ=255) gTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCgcag > 2:707653/1‑150 (MQ=255) cgcgCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGa > 2:375841/1‑147 (MQ=255) cgcgCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGa < 1:375841/147‑1 (MQ=255) aCTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCCCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAagc < 1:1058572/150‑1 (MQ=255) ggcggcTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCACCTCCa > 2:281302/1‑150 (MQ=255) ggcTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCg < 1:561956/150‑1 (MQ=255) tGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAag < 1:351671/136‑1 (MQ=255) tGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAag > 2:351671/1‑136 (MQ=255) cGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCAc > 2:1006169/1‑150 (MQ=255) cGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCAc < 2:903547/150‑1 (MQ=255) gAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGAAAAAGCAGCTCCAGCGTTTGCACg > 2:880304/1‑150 (MQ=255) gCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGa > 1:1026059/1‑150 (MQ=255) cTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTcgcg > 1:246723/1‑150 (MQ=255) aaCAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTgg < 1:724215/150‑1 (MQ=255) cctcctTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGgccgcc > 2:37400/1‑150 (MQ=255) tgtttcAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGG‑TTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCgg < 1:837013/145‑1 (MQ=255) aGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCgcgc > 2:267471/1‑121 (MQ=255) aGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCgcgc < 1:267471/121‑1 (MQ=255) ccACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCATcgac < 1:409400/150‑1 (MQ=255) ttGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCATCGACGATg > 2:728671/1‑150 (MQ=255) ccccAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCATCGACGATGCCGt > 1:825110/1‑150 (MQ=255) cccAGGGCATCGAT‑CCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGc > 2:903371/1‑135 (MQ=255) cccAGGGCATCGAT‑CCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGc < 1:903371/135‑1 (MQ=255) cccAGGGCATCGAT‑CCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCATCGACGATGCCGTa > 1:796287/1‑150 (MQ=255) cccAGGGCATCGAT‑CCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTAAGCCAGAGGTCTCGCTGGCCGCCCGCCCGCGTCGTTCGGCATCGACGATGCCGTa > 2:31745/1‑150 (MQ=255) cccAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCATCGACGATGCCGTa < 1:728671/150‑1 (MQ=255) cccAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCATCGACGATGCCGTa > 2:796993/1‑150 (MQ=255) ccAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCATCGACGATGCCGTac > 1:500255/1‑150 (MQ=255) cAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGcgtcgt < 1:1152030/128‑1 (MQ=255) cAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGcgtcgt > 2:1152030/1‑128 (MQ=255) tCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCATCGACGATGCCGTACACCATGc > 1:321422/1‑150 (MQ=255) cGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCATCGACGATGCCGTACACCATGCt < 2:790685/150‑1 (MQ=255) | GAACGAAGCAGCCTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCATCGACGATGCCGTACACCATGCT > NC_002947/3218564‑3218856 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |