| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,826,490 | Δ1 bp | 12.2% | coding (4745/12954 nt) | pvdL ← | non‑ribosomal peptide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,826,490 | 0 | T | . | 12.2% | 113.2 / 11.8 | 41 | coding (4745/12954 nt) | pvdL | non‑ribosomal peptide synthetase |
| Reads supporting (aligned to +/- strand): ref base T (18/18); new base . (3/2); total (21/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.41e-01 | |||||||||||
TGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTGCTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGG > NC_002947/4826343‑4826635 | tgttgCAGCTCGGTTCGTGGCTCGACATGCTCACGCTGCTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCa < 2:584342/150‑1 (MQ=255)tgttgCAGCTCGGTTCGTGGCTCGACATGCTCACGCTGCTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCa < 1:508714/150‑1 (MQ=255) gCTCACGCTGCTGCCACACTGGGGCAGGCAGTATGGGTCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATCTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCt > 1:1188956/1‑150 (MQ=255) cTCACGCTGCTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTg < 1:371820/150‑1 (MQ=255) tCACGCTGCTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTgg < 2:756623/150‑1 (MQ=255) tgcctgcCCCAGTGTGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCCGGGGCCCAATGGCATCTGTGCCAAAGGGCTGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCt > 2:402924/4‑150 (MQ=255) tGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATa > 2:38114/1‑150 (MQ=255) gcaggcagTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCg > 1:1098179/1‑150 (MQ=255) gcagTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCa > 1:1027066/1‑150 (MQ=255) gcagTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCa > 2:757261/1‑150 (MQ=255) cagTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGc < 1:1058004/105‑1 (MQ=255) cagTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGc > 2:1058004/1‑105 (MQ=255) cTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTgcgc < 1:38114/150‑1 (MQ=255) cGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATgg < 1:757261/150‑1 (MQ=255) ttGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTcc > 2:908039/1‑150 (MQ=255) gCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCtt < 1:776614/150‑1 (MQ=255) cGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTtgt < 2:417517/150‑1 (MQ=255) cGCTGGGGACCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTtgt > 2:380311/1‑150 (MQ=255) ggggCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTAt < 1:908039/150‑1 (MQ=255) gggCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATc < 2:1027066/150‑1 (MQ=255) gggCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATc > 2:228835/1‑150 (MQ=255) gCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTtg > 1:163393/1‑134 (MQ=255) gCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTtg < 2:163393/134‑1 (MQ=255) aTCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGcc > 2:28781/1‑150 (MQ=255) gAGTTGCGCCGGCACCAGGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCtg < 2:1098179/150‑1 (MQ=255) gTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCtgtg > 1:140287/1‑150 (MQ=255) ttGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGc > 1:458210/1‑83 (MQ=255) ttGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGc < 2:458210/83‑1 (MQ=255) tggctgagCAATTGC‑CAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCa > 1:995640/8‑150 (MQ=255) tggctgagCAATTGC‑CAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCa > 1:164616/8‑150 (MQ=255) aGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTtgt > 2:617340/1‑96 (MQ=255) aGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTtgt < 1:617340/96‑1 (MQ=255) cTGGCAATTGCTCAGCGAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCCGCAGGCAGGCCTGCACtt > 1:41545/1‑150 (MQ=255) tGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTc < 1:437107/150‑1 (MQ=255) gagCAATTGC‑CAGCCAGTACGGCCAGCACCTCGGCAGGCTggg > 2:387131/3‑43 (MQ=255) gagCAATTGC‑CAGCCAGTACGGCCAGCACCTCGGCAGGCTggg < 1:387131/41‑1 (MQ=255) gCAATTGC‑CAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTc < 2:164616/150‑1 (MQ=255) aaTTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCg > 2:501747/1‑150 (MQ=255) aTTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTtgt > 2:671815/1‑87 (MQ=255) aTTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTtgt < 1:671815/87‑1 (MQ=255) ttGCTCACCAAGTACGGGTAGCGCTTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTAACATCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCggg < 1:380311/150‑1 (MQ=255) | TGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTGCTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAATTGCTCAGCCAGTACGGCCAGCACCTCGGCAGGCTGGGCGCTGCCACTGTAATAGCCGACCAGTTGCGCGCCAATGGCGTCCTTGTGTATCAGCACCAGCGCCTGTTCCACACCCGCCTGTGCCAGCAGGCAGGCCTGCACTTCCTCGGG > NC_002947/4826343‑4826635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |