| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,835,842 | Δ1 bp | 8.9% | coding (147/693 nt) | PP_4249 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,835,842 | 0 | C | . | 8.9% | 129.8 / 8.8 | 45 | coding (147/693 nt) | PP_4249 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (18/22); new base . (2/2); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01 | |||||||||||
TTCAAGCAACACCTTCTGCGGGTTCGGCGGCCGCTTGCCACCGGTAACCCTGCGCTCGGCCATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGCCCATTGATCCCCGTCAATACCGGCACTTTGCCCATTAATCATGCT > NC_002947/4835698‑4835991 | ttCAAGCAACACCTTCTGCGGGTTCGGCGGCCGCTTGCCACCGGTAACCCTGCGCTCGGCCATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGgcgc < 2:524413/150‑1 (MQ=255) gCAACACCTTCTGCGGGTTCGGCGGCCGCTTGCCACCGGTAACCCTGCGCTCGGCCATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGcgcgcccg > 1:323429/1‑150 (MQ=255) tGCGGGTTCGGCGGCCGCTTGCCACCGGTAACCCTGCGCTCGGCCATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGcc < 1:175663/150‑1 (MQ=255) gggTCCGGCGGCCGCTTGCCACCGGTAACCCTGCGCTCGGCCATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGa > 2:933240/1‑150 (MQ=255) gTTCGGCGGCCGCTTGCCACCGGTAACCCTGCGCTCGGCCATGTCCGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATGTCGTCCATAAACCCGCTGTCCAGCTGCGCGCGCGCGCGATTGTCCAGaaa > 2:1164306/1‑149 (MQ=255) tCGGCGGCCGCTTGCCACCGGTAACCCTGCGCTCGGCCATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCa < 2:323429/150‑1 (MQ=255) cggcggCCGCTTGCCACCGGTAACCCTGCGCTCGGCCATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAg < 1:933240/150‑1 (MQ=255) ggcggcCGCTTGCCACCGGTAACCCTGCGCTCGGCCATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCaag < 2:1027353/150‑3 (MQ=255) cGCTTGCCACCGGTAACCCTGCGCTCGGCCATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCCCGCCATGGGCCAGAATCAGGCAcccc > 2:880979/1‑150 (MQ=255) aCCGGTAACCCTGCGCTCGGCCATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTc < 2:86558/150‑1 (MQ=255) cccTGCGCTCGGCCATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTACGTCCATGAACCCGCTGTCCAT‑GGcgcgggcgcgccgatg > 2:362839/1‑111 (MQ=255) cccTGCGCTCGGCCATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTACGTCCATGAACCCGCTGTCCAT‑GGcgcgggcgcgccgatg < 1:362839/115‑5 (MQ=255) cTGCGCTCGGCAATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCt < 1:849601/150‑1 (MQ=255) ggCCATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCcg > 1:333287/1‑150 (MQ=255) ccATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCcgcg < 2:333287/150‑1 (MQ=255) aTGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCgg‑‑a < 1:996016/150‑1 (MQ=255) tACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCgg‑‑agga < 1:498163/150‑1 (MQ=255) tCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCAT‑GGcgcgggcgcgccgatg < 1:52968/89‑5 (MQ=255) tCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCAT‑GGcgcgggcgcgccgatg > 2:52968/1‑85 (MQ=255) gAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCaa > 1:356724/1‑150 (MQ=255) cgcACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCt < 1:108545/150‑1 (MQ=255) aCTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTggg < 1:5871/150‑1 (MQ=255) tACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGAc > 2:940333/1‑150 (MQ=255) aCCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAgc > 1:157084/1‑136 (MQ=255) aCCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAgc < 2:157084/136‑1 (MQ=255) ccAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGAcc > 1:123334/1‑150 (MQ=255) cAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTTCGACCt > 1:505657/1‑150 (MQ=255) agCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGc < 2:505657/150‑1 (MQ=255) gCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGcc < 1:632980/150‑1 (MQ=255) ccGCAAGCCTTTGCGCCATTTCGGCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGccc < 1:1164306/150‑1 (MQ=255) cGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGCCCa > 1:903519/1‑150 (MQ=255) gCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATAGGCGCGCCCGCACGAATGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGCCCAt > 1:751434/1‑150 (MQ=255) aaGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCAt > 1:652829/1‑86 (MQ=255) aaGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCAt < 2:652829/86‑1 (MQ=255) ccTTTGCCCCATTTCGTCCATGAGCCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCCACCCGCGG‑‑AGGATCGCAGCGCCAGCGTGCGACCTTTGCCACCTCCGCCCagtttcc < 2:620702/150‑7 (MQ=255) tttGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGCCCATTGATccc > 1:518497/1‑150 (MQ=255) tCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGCCCATTGATCCCCGTCAATACCGGCa > 2:120981/1‑150 (MQ=255) ccATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGCCCATTGATCCCCGTCAATACCGGCAc > 1:773941/1‑150 (MQ=255) ccATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGCCCATTGATCCCCGTCAATACCGGCAc < 2:751434/150‑1 (MQ=255) ccATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGCCCATTGATCCCCGTCAATACCGGCAc < 1:120981/150‑1 (MQ=255) ccGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGGTCTGCAT‑G‑TGCCCAGGCCGCGGACATTTTCGACATTGGTCGATTGAGCCCCGGCAATACCGGCACTTTGCCCa > 1:928817/1‑150 (MQ=255) cGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGCCCATTGATCCCCGTCAATACCGGCACTTTGCCCAt < 1:940333/150‑1 (MQ=255) cGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGCCCATTGATCCCCGTCAATACCGGCACTTTGCCCAt > 1:138471/1‑150 (MQ=255) cATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGCCCATTGATCCCCGTCAATACCGGCACTTTGCCCATTAATCAt > 2:1057446/1‑150 (MQ=255) aTCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGCCCATTGATCCCCGTCAATACCGGCACTTTGCCCATTAATCATg < 1:619135/150‑1 (MQ=255) cGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGCCCATTGATCCCCGTCAATACAGGCACTTTGCCCATTAATCATGCt > 1:733259/1‑150 (MQ=255) | TTCAAGCAACACCTTCTGCGGGTTCGGCGGCCGCTTGCCACCGGTAACCCTGCGCTCGGCCATGTACGGGAACTCGAAGCGCACTACCGCTACCCCAAGAGCCGCAAGCCTTTGCGCCATTTCGTCCATGAACCCGCTGTCCATCGGCGCGCCCGCGCCATGGGCCAGAATCAGGCACCCCTTGTAGTCATCCTGACCTGCAACCCGCGG‑‑AGGATCGCAGCGCAAGCCTGGGACATTTGCGACCTTCGCCCATTGATCCCCGTCAATACCGGCACTTTGCCCATTAATCATGCT > NC_002947/4835698‑4835991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |