Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,697,369:1 +A 9.2% intergenic (+23/‑54) hslV → / → hslU ATP‑dependent HslVU protease peptidase subunit/protease HslVU ATPase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,697,3691.A 9.2% 232.6 / 19.7 65intergenic (+23/‑54)hslV/hslUATP‑dependent HslVU protease peptidase subunit/protease HslVU ATPase subunit
Reads supporting (aligned to +/- strand):  ref base . (31/28);  new base A (4/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.91e-01

CCCTGCTGAACAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTGCGCAAC  >  NC_002947/5697222‑5697518
                                                                                                                                                    |                                                                                                                                                     
cccTGCTGAACAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTc‑cc                                                                                                                                                     <  1:1140816/150‑1 (MQ=255)
 ccTGCTGAACAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCg                                                                                                                                                    >  1:845176/1‑150 (MQ=255)
 ccTGCTGAACAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCg                                                                                                                                                    >  2:676705/1‑150 (MQ=255)
   tgctgAACAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGt                                                                                                                                                  >  2:1175806/1‑150 (MQ=255)
        aaCAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAgg                                                                                                                                             <  1:685999/150‑1 (MQ=255)
           aaGACACACCTGTCGGCCCGCGAAATTGCAGAGATCGCCCTGAACATCGCCGGCGACATATGCGTGTTCACCAACCACACCCTGCCCTTCGAGGAGGAGGACCTGGCCGACTGATTCGGCTGTTCTGGCGCCCCTTC‑ACCGGGAAGGGGc                                                                                                                                          <  2:26126/150‑1 (MQ=255)
            ggaccgaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑ACCGGGAAGGGGcg                                                                                                                                         <  2:6328/149‑2 (MQ=255)
             gaccgaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCtt                                                                                                                                        >  2:802212/1‑150 (MQ=255)
             gaccgaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑ACCGGGAAGGGGcgc                                                                                                                                        <  1:175101/150‑3 (MQ=255)
             gaccgaccTCTCGGCCCGCGAAATCGCCGAGACAGCCCTGAAAATCGCCGGCGACATCTGCGTGTCCCCCAACCACAACCTGACCATCGAGGATCCGAACCTGGCCGCCTGAACCACCCGTTCACGCCCTCCTTC‑CCGGTGAAGGGGcgg                                                                                                                                        >  2:561315/1‑148 (MQ=255)
              accgaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCttt                                                                                                                                       <  1:1175806/150‑1 (MQ=255)
               ccgaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCtttt                                                                                                                                      >  1:277417/1‑150 (MQ=255)
                   ccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCAc                                                                                                                                  <  1:802212/150‑1 (MQ=255)
                     tctcGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGc                                                                                                                                >  1:1065898/1‑150 (MQ=255)
                       tcGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGc                                                                                                                                >  2:472892/1‑148 (MQ=255)
                       tcGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGc                                                                                                                                <  1:472892/148‑1 (MQ=255)
                           cccGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTc                                                                                                                          >  2:791083/1‑150 (MQ=255)
                            ccGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCa                                                                                                                         >  2:909436/1‑150 (MQ=255)
                                     cGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCaa                                                                                                                <  2:176866/150‑1 (MQ=255)
                                        cGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGCCTGATTCAGCTGTTCTGGCTCCGCAAC‑CGCGTTAAGTTGCTTTTCCACGCTGACTCACCTGCCCAAGGa                                                                                                             <  1:1114485/150‑1 (MQ=255)
                                               gCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCACCGG‑GAAGGGGc                                                                                                                                          <  2:928100/114‑1 (MQ=255)
                                               gCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATACAGCTGTTCTGGCGCCCCTTCACCGG‑GAAGGGGc                                                                                                                                          >  1:928100/1‑114 (MQ=255)
                                                 ccTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGa                                                                                                    <  1:676705/150‑1 (MQ=255)
                                                 ccTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGa                                                                                                    >  2:630876/1‑150 (MQ=255)
                                                  cTGAACCTCGCCGGCGCTAGCTTCGTGTGCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGAt                                                                                                   <  2:374490/150‑1 (MQ=255)
                                                        aTCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTc                                                                                             >  1:28270/1‑150 (MQ=255)
                                                           gCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCAt                                                                                          <  2:277417/150‑1 (MQ=255)
                                                           gCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCAt                                                                                          <  1:909436/150‑1 (MQ=255)
                                                           gCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATATCCAt                                                                                          <  2:340265/150‑1 (MQ=255)
                                                             cGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGa                                                                                        <  1:974182/150‑1 (MQ=255)
                                                                     tCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCcgc                                                                                <  2:791516/150‑1 (MQ=255)
                                                                        gCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCgag                                                                             <  1:791083/150‑1 (MQ=255)
                                                                                accaacaaCACCCTGACCATCTAGGAGAAGGACCTGGCCAACTGGTTCAGGTGTTCTGGCGCCCCTTC‑CCGGGGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTcaa                                                                     >  2:1200201/1‑148 (MQ=255)
                                                                                 ccaaccaCAACTTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGAGCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCAc                                                                    >  2:438957/1‑150 (MQ=255)
                                                                                 ccaaccaCAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAg                                                                                                               >  2:247142/1‑107 (MQ=255)
                                                                                 ccaaccaCAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAg                                                                                                               <  1:247142/107‑1 (MQ=255)
                                                                                  caaccaCAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑ACCGGGAAGGGGc                                                                                                                                          <  2:847328/79‑1 (MQ=39)
                                                                                  caaccaCAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑ACCGGGAAGGGGc                                                                                                                                          >  1:847328/1‑79 (MQ=39)
                                                                                    accaCAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTCTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGCTAATGTCCCTAACCGCCCGCAATAGTGTCCAGGaa                                                                 >  2:1163697/1‑150 (MQ=255)
                                                                                      cacaACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGGTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACt                                                               >  2:1137134/1‑150 (MQ=255)
                                                                                       acaACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGAcccccc                                                                                  >  1:538210/1‑130 (MQ=255)
                                                                                       acaACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGAcccccc                                                                                  <  2:538210/130‑1 (MQ=255)
                                                                                          aCCTGACCATCGAGGAGCAGGACCTGACCGACTGATTCAGCTGTTCTGGCGAACCTTC‑CCGGTGAAGGGGCTTTTCGACGCTGACTCACCTGCCCAACGCCCGTATTAATCATCTCCATGACCCCCCGCGACATCCTCCACGGACTCAAc                                                           >  2:295457/1‑150 (MQ=255)
                                                                                                 cATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCccca                                                    <  1:1137134/150‑1 (MQ=255)
                                                                                                 cATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTACTGGCACCCATTC‑CAGGAAAAGCGACACTAAAAAGCCGACTAACTATCACAATAACAGTATGGATGAGGGCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCcaca                                                    >  2:439369/1‑150 (MQ=255)
                                                                                                 cATCGAGGAGCAGGACCCGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCcaca                                                    >  2:619348/1‑150 (MQ=255)
                                                                                                   ccGAGGAGCACGACCTCGCCGACTGATTCACCCCTTCTGGCGCCCCTTC‑CCGGGGAAGGGGCTTTTCCACGCTTACTCACCTGCCCAAGGACCGTTTTGTTTTTATCCAACACCCCCCACCGAAACCTCCACGAACTCAACCGCCAcatc                                                  <  2:388732/149‑1 (MQ=255)
                                                                                                      aggagAAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACatcatc                                               <  2:388924/150‑1 (MQ=255)
                                                                                                           cAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCa                                          <  2:845176/150‑1 (MQ=255)
                                                                                                              gACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGGATTGCTCATGTCCACGACCCCCCGCGAGATCGTCCACTAACTCAACCGCCACATCATCGGCCAggc                                       >  2:436249/1‑149 (MQ=255)
                                                                                                                          tGATTCAGATGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAgcg                           >  2:1201055/1‑150 (MQ=255)
                                                                                                                              tCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGAACATGTCCATGACCCCCCGCGAGCTCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGcc                       >  1:640761/1‑150 (MQ=255)
                                                                                                                                   tGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCa                                                                                           >  1:787260/1‑77 (MQ=255)
                                                                                                                                   tGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCa                                                                                           <  2:787260/77‑1 (MQ=255)
                                                                                                                                   tGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGt                                                                        <  2:233994/96‑1 (MQ=255)
                                                                                                                                   tGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGt                                                                        >  1:233994/1‑96 (MQ=255)
                                                                                                                                         ggCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGc            <  1:903780/150‑1 (MQ=255)
                                                                                                                                          gcgcCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGCGGCCATTGcc           >  2:662378/1‑150 (MQ=255)
                                                                                                                                          gcgcCCCTTC‑CCGGTGAAGGGGCTGTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTTCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGcc           >  1:226712/1‑150 (MQ=255)
                                                                                                                                            gcCCCTTCACCGG‑GAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCaa                              >  1:1198974/1‑129 (MQ=255)
                                                                                                                                            gcCCCTTCACCGG‑GAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCaa                              <  2:1198974/129‑1 (MQ=255)
                                                                                                                                            gcCCCTTCACCGG‑GAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTg                    >  1:890493/1‑139 (MQ=255)
                                                                                                                                            gcCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCt         >  1:45589/1‑150 (MQ=255)
                                                                                                                                            gcCCCTTC‑ACCGGGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTg                    <  2:890493/139‑1 (MQ=255)
                                                                                                                                             ccccTTCACCGG‑GAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTg        >  1:183836/1‑150 (MQ=255)
                                                                                                                                                 ttC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCCCCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTGCGCa    >  1:1189133/1‑150 (MQ=255)
                                                                                                                                                   c‑ccGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTGCGCAAc  >  1:959717/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCCTGCTGAACAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTC‑CCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTGCGCAAC  >  NC_002947/5697222‑5697518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑