| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,758,222:1 | +C | 9.4% | intergenic (+190/+328) | PP_5050 → / ← PP_5051 | transposase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,758,222 | 1 | . | C | 9.4% | 200.6 / 14.6 | 53 | intergenic (+190/+328) | PP_5050/PP_5051 | transposase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (24/24); new base C (2/3); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATCCAATGCTGGGGAGAAATTCTCTGGCACGCTGACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCACCCGCAAAGCCCAGTATTCACGGCTTTTGCAAAACTGGCA > NC_002947/5758073‑5758360 | tATCCAATGCTGGGGAGAAATTCTCTGGCACGCTGACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGgc > 2:866742/1‑150 (MQ=255) tCCAATGCTGGGGAGAAATTCTCTGGCACGCTGACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGgc‑gc > 2:769805/1‑150 (MQ=255) tGGGGAGAAATTCTCTGGCACGCTGACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGc < 1:1122563/150‑1 (MQ=255) tGGGGAGAAATTCTCTGGCACGCTGACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGc < 1:13167/150‑1 (MQ=255) agaAATTCTCTGGCACGCTGACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAg > 2:222651/1‑150 (MQ=255) ggcgtgccagttttgcaaaagccgtgaatactgggctttgcgggtgatttggccggattttgaggggatttgtgccttccagaggtgcagcgggcacaggaacggtgcattcagacgactgaaatggcTGGGGC‑GCAAAGCGGCCCCAGc > 2:61733‑M1/129‑150 (MQ=255) tctGGCACGCTGACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGAGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCa < 2:742052/150‑1 (MQ=255) gttttgcaaaagccgtgaatactgggctttgcgggtgatttggccggattttgaggggatttgtgccttccagaggtgcagcgggcacaggaacggtgcattcagacgactgaaatggcTGGGGC‑GCAAAGCGGCCCCa < 2:607943‑M1/20‑1 (MQ=255) gttttgcaaaagccgtgaatactgggctttgcgggtgatttggccggattttgaggggatttgtgccttccagaggtgcagcgggcacaggaacggtgcattcagacgactgaaatggcTGGGGC‑GCAAAGCGGCCCCa > 1:607943‑M1/120‑139 (MQ=255) cACGCTGACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAgtcgt < 2:1052585/150‑1 (MQ=255) aCGCTGACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAgtcgtc < 2:1081934/150‑1 (MQ=255) aCGCTGACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAgtcgtc < 1:222651/150‑1 (MQ=255) cGCTGACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCt > 2:308056/1‑150 (MQ=255) gACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAAt < 1:769805/150‑1 (MQ=255) tCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCgg > 1:71674/1‑122 (MQ=255) tCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCgg < 2:71674/122‑1 (MQ=255) tCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCt > 2:236024/1‑143 (MQ=255) tCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCt < 1:236024/143‑1 (MQ=255) cTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTc > 1:553245/1‑150 (MQ=255) cTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTc > 2:688218/1‑150 (MQ=255) cTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTc > 2:878593/1‑150 (MQ=255) tCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAgtcgtc < 2:361175/135‑1 (MQ=255) tCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAgtcgtc > 1:361175/1‑135 (MQ=255) cGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGACAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCt < 1:1157118/150‑1 (MQ=255) tGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAgtcgt < 1:1078826/124‑1 (MQ=255) tGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAgtcgt > 2:1078826/1‑124 (MQ=255) agGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACct > 1:846348/1‑150 (MQ=255) cGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTgg > 1:668302/1‑150 (MQ=255) aTGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGaa < 2:553245/150‑1 (MQ=255) aaaaTTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTc > 1:656490/1‑150 (MQ=255) cTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAAt < 2:590912/94‑1 (MQ=255) cTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAAt > 1:590912/1‑94 (MQ=255) tCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGc < 2:268662/150‑1 (MQ=255) tCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGc > 2:369679/1‑150 (MQ=255) ccATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAAt < 2:656490/150‑1 (MQ=255) tAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCAc > 1:234951/1‑150 (MQ=255) ccccgAATCCTGTGGGAGCGGCCTTCTGTCGCGATGGGGC‑GCAAATCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGGAGGCACAAATCCCCTCAAAATCCGGCCAAATCACCCGCAAAGccc < 2:574824/149‑1 (MQ=255) cccAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCGCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCg > 1:1124302/1‑127 (MQ=255) cccAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACctct > 1:716782/1‑100 (MQ=255) cccAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACctct < 2:716782/100‑1 (MQ=255) cccAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCg < 2:1124302/127‑1 (MQ=255) ccAATCCTGGGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGGGGGCGCACATACTTTAGACGACTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCTCTCAACATCCGGCCAAATCACCCGCAAAGCCCAGt > 2:644764/1‑150 (MQ=255) cAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCACCCGCAAAGCCCAGTa > 1:927334/1‑150 (MQ=255) ccTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCACCCGCAAAGCCCAGTATTCa < 1:878593/150‑1 (MQ=255) attcagacgactgaaatggcTGGGGCCGCTTTGCG‑CCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCACCCGCAAAGCCCa > 2:1085226‑M1/21‑137 (MQ=255) attcagacgactgaaatggcTGGGGCCGCTTTGCG‑CCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCACCCGCAAAGCCCa < 1:1085226‑M1/117‑1 (MQ=255) aGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCACCCGCAAAGCCCAGTATTCACGGCtttt < 2:927334/150‑1 (MQ=255) gCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCACCCGCAAAGCCCAGTATTCACGGCTTTTg < 2:668302/150‑1 (MQ=255) ccTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCACCCGCAAAGCCCAGTATTCACGGCTTTTGCaaa > 2:1050075/1‑150 (MQ=255) gactgaaatggcTGGGGCCGCTTTGCG‑CCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCACCCGCAAAGCCCa < 2:307823‑M1/117‑1 (MQ=255) gactgaaatggcTGGGGCCGCTTTGCG‑CCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCACCCGCAAAGCCCa > 1:307823‑M1/13‑129 (MQ=255) tgaaatggcTGGGGCCGCTTTGCG‑CCCCATCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCACCCGCAAAGCCCAGTATTCACGGCTTTTGCAAAACTg < 2:1183810‑M1/141‑1 (MQ=255) tCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCACCCGCAAAGCCCAGTATTCACGGCTTTTGCAAAACTGGCa > 1:1023855/1‑150 (MQ=255) C‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCAc < 2:184810‑M1/100‑1 (MQ=255) C‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCAc > 1:184810‑M1/1‑100 (MQ=255) | TATCCAATGCTGGGGAGAAATTCTCTGGCACGCTGACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAATCTCCTACCCCAATCCTGTGGGAGCGGCCTTGTGTCGCGATGGGGC‑GCAAAGCGGCCCCAGCCATTTCAGTCGTCTGAATGCACCGTTCCTGTGCCCGCTGCACCTCTGGAAGGCACAAATCCCCTCAAAATCCGGCCAAATCACCCGCAAAGCCCAGTATTCACGGCTTTTGCAAAACTGGCA > NC_002947/5758073‑5758360 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |