| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 70,755:1 | +G | 8.3% | coding (1150/2055 nt) | glyS ← | glycine‑tRNA ligase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 70,755 | 1 | . | G | 8.3% | 183.2 / 9.4 | 48 | coding (1150/2055 nt) | glyS | glycine‑tRNA ligase subunit beta |
| Reads supporting (aligned to +/- strand): ref base . (24/20); new base G (2/2); total (26/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGCGGCATGTACTGCTCGTTCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGG > NC_002947/70608‑70899 | cgcgcgGCATGTACTGCTCGTTCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑a > 2:729306/1‑149 (MQ=255)cgcgcgGCATGTACTGCTCGTTCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑a < 1:729306/149‑1 (MQ=255) tGCTCGTTCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGCGACGCCCAGCGCGCTg > 1:453612/1‑150 (MQ=255) cTCGTTCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGc > 2:829779/1‑150 (MQ=255) cTCGTTCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGc > 2:829976/1‑150 (MQ=255) cTCGTTCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGc > 1:408696/1‑150 (MQ=255) ttCAGGTCCAGGACTACGTCTTCCGGTTCACCGTCGGTGGGCGTGTAGTAGTAAACGGCAACGCCCTGCGTCTCAGGGAATTCGCGGACCATCGCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGGCAGCGCGCTGGtcgtcg > 2:961888/1‑150 (MQ=255) tCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGCGACGCCCAGCGCGCTGGCCGtgc < 2:453612/150‑3 (MQ=255) cagtgccaggtcTACGTCTCCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATGCGCCGACCATCTCGGTGGCCANGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTcgcc < 1:623187/150‑1 (MQ=255) gggccagggcTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTcgccgc > 2:1106757/1‑150 (MQ=255) gccagggcTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGc > 1:540187/1‑140 (MQ=255) gccagggcTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGc < 2:540187/140‑1 (MQ=255) gcTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGc > 2:912754/1‑150 (MQ=255) aCCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGACCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGACCAATGAAgccggc > 2:431498/1‑150 (MQ=255) gcgTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGCGACGCCCAGCGCGCTGGCCGt > 1:245386/1‑107 (MQ=255) gcgTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGCGACGCCCAGCGCGCTGGCCGt < 2:245386/107‑1 (MQ=255) gcgTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAaca > 2:211370/1‑150 (MQ=255) gtagtagtaACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGGCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACg > 2:362104/1‑150 (MQ=255) aaCCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCt > 1:902945/1‑150 (MQ=255) aCCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCt < 1:601561/89‑1 (MQ=255) aCCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCt > 2:601561/1‑89 (MQ=255) ggCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCCCTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAACAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTc < 1:1149976/150‑1 (MQ=255) ggCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTc < 1:829779/150‑1 (MQ=255) ggCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTc < 1:912754/150‑1 (MQ=255) ggCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTc < 1:829976/150‑1 (MQ=255) cccTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTAAACg < 2:1121672/150‑1 (MQ=255) ccTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACgg > 1:820017/1‑150 (MQ=255) tGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGa < 2:115869/99‑1 (MQ=255) tGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGa > 1:115869/1‑99 (MQ=255) tttCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGa < 2:902945/150‑1 (MQ=255) ttCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCg < 2:1067294/127‑1 (MQ=255) ttCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCg > 1:1067294/1‑127 (MQ=255) aGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCg < 1:959379/124‑1 (MQ=255) aGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCg > 2:959379/1‑124 (MQ=255) aaTTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGcc > 2:969578/1‑150 (MQ=255) ttCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTg < 2:408696/150‑1 (MQ=255) gCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGaa < 2:486528/150‑1 (MQ=255) ccATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAAcacca > 2:174021/1‑150 (MQ=255) ggTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGACGGCGGCCCGCAAGGCCAAGCGTTATTCCTGGTCGGGGACGGTGCCGTGCTGAGCCTGGAACACCACGTTCtt > 1:871799/1‑150 (MQ=255) gTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTg < 1:211370/150‑1 (MQ=255) gCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCGCGTTCTTGAgg < 1:969578/150‑1 (MQ=255) ggTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCt > 2:725232/1‑150 (MQ=255) gTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTc > 2:448083/1‑150 (MQ=255) ttCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAg > 1:365243/1‑150 (MQ=255) gACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGaa < 2:1205247/146‑1 (MQ=255) gACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGaa > 1:1205247/1‑146 (MQ=255) caCAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCAAATTTTCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGtt < 1:316905/149‑1 (MQ=255) aCAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGttt > 1:786690/1‑150 (MQ=255) agGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGt < 1:638536/150‑1 (MQ=255) gCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTgg < 1:1106757/150‑1 (MQ=255) | CGCGCGGCATGTACTGCTCGTTCAGGGCCAGGGCTACGTCTTCCGGCTCACCGTCGTTGAGCGCGTAGTAGTAACCGGCAACACCCTGCATTTCAGGGAATTCGCCGACCATCTCGGTGGCCAGGTCGCACTTCGACAGCAGGCCGGC‑ACGGCCAGCGCGCTGGGCGTCGCCGCCGATCAGCGGAGCAATGAAGGCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGG > NC_002947/70608‑70899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |