| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 702,766:1 | +G | 10.0% | intergenic (+11/‑121) | PP_23SD → / → PP_5SD | 23S ribosomal RNA/5S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 702,766 | 1 | . | G | 10.0% | 144.9 / 16.1 | 50 | intergenic (+11/‑121) | PP_23SD/PP_5SD | 23S ribosomal RNA/5S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base . (23/22); new base G (2/3); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01 | |||||||||||
ACTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAA > NC_002947/702630‑702912 | aCTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTA‑GCAGATTGct > 1:1093001/1‑149 (MQ=2) cTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTA‑GCAGATTGctt < 2:1093001/150‑3 (MQ=2) gggATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTA‑GCAGATTGcttgg < 1:104463/150‑5 (MQ=11) gggATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCggtg < 2:267698/150‑1 (MQ=32) gATCTTGAATCCCCTGAAGGGTCGTCGAAGACTACGACGTTGNTAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAACAATCTGCGTA‑GCAGATTGcttgggt < 2:220742/150‑7 (MQ=11) tCTTGAATCCCCTAAAGGGCCGTCGAANACTACGACGTTGNTAGGTTNGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGa > 1:1180691/1‑150 (MQ=17) ccccTAAAGGGCGGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGaaa < 2:525879/150‑1 (MQ=17) ccacTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGAAGaaa < 2:750710/147‑1 (MQ=17) ccTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGACGTTGAGCTAACCAATTCCAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGa > 1:147103/1‑150 (MQ=12) cTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGAc > 1:454813/1‑150 (MQ=32) gCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGaaaa < 1:842341/150‑1 (MQ=32) gTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTc > 1:91827/1‑150 (MQ=32) cGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGt < 2:1180691/150‑1 (MQ=32) cGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGt < 2:454813/150‑1 (MQ=32) tACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTac > 1:858269/1‑150 (MQ=32) gacgTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTAcaca > 1:465314/1‑150 (MQ=32) gTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCg < 2:389264/99‑1 (MQ=32) gTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCg > 1:389264/1‑99 (MQ=32) gggTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACACAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAat < 1:944133/150‑1 (MQ=17) aaGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCata > 1:524224/1‑150 (MQ=32) gcgcTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATc > 1:765052/1‑150 (MQ=32) cgcTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATcc < 2:858269/150‑1 (MQ=32) cTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGa < 2:465314/150‑1 (MQ=32) gCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCg < 1:592161/105‑1 (MQ=33) gCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCg > 2:592161/1‑105 (MQ=33) gCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCt < 1:881339/150‑1 (MQ=32) ttGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTGGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTggg > 1:1057681/1‑150 (MQ=17) gCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGt < 2:91827/150‑1 (MQ=32) cTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTc > 2:674627/1‑150 (MQ=32) aCCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCAt > 2:1119811/1‑150 (MQ=32) aCCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCAt < 2:765052/150‑1 (MQ=32) cAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCt < 1:674627/150‑1 (MQ=32) aCTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCg > 1:525484/1‑117 (MQ=33) aCTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCg < 2:525484/117‑1 (MQ=33) cccGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCCAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGc < 1:178436/150‑1 (MQ=17) cGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCaa > 2:893238/1‑150 (MQ=32) tGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACa < 2:147103/150‑1 (MQ=32) cgtGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAAtt > 2:174132/3‑150 (MQ=17) gACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCt > 2:1120041/1‑150 (MQ=32) atatAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACa > 2:1135298/1‑127 (MQ=33) atatAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACa < 1:1135298/127‑1 (MQ=33) acCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCtt > 1:304832/1‑140 (MQ=33) acCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCtt < 2:304832/140‑1 (MQ=33) ccAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGc > 1:933023/1‑104 (MQ=32) ccAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGc < 2:933023/104‑1 (MQ=33) cAAGCAATCTGC‑TACGCAGATGGCGGTTGTGAAGCCGAAAGACCCGAAAATTCGTGCTTTACACACGAGCTTCGCAAATATCGCAGACCCGAATTCGCTGGGCTGTCCGTCTGAACATTCTGGCAAAAGAATTTCTTGACGACCATagag > 2:445885/1‑150 (MQ=11) aaGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGc > 1:392398/1‑150 (MQ=32) cgCAATCTGCGTA‑GCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGAAGCAAGCGCATATCCGAATTCGCTGGGCTGTCGATCTGGACATGCTGGCAACAGAATTTCTTGACGACCATAGAGCa > 1:870948/2‑150 (MQ=11) cAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCAtt < 1:893238/150‑1 (MQ=32) cTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGaa > 2:692343/1‑150 (MQ=32) | ACTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAA > NC_002947/702630‑702912 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |