| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 715,012:1 | +T | 13.5% | intergenic (+75/+2) | ispH → / ← PP_0607 | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase/type IV pili biogenesis protein FimT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 715,012 | 1 | . | T | 13.5% | 116.0 / 16.5 | 37 | intergenic (+75/+2) | ispH/PP_0607 | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase/type IV pili biogenesis protein FimT |
| Reads supporting (aligned to +/- strand): ref base . (14/18); new base T (2/3); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
AAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTGCGCCCAACCAGCTGTTGCCCGACGTCATGGGC > NC_002947/714864‑715158 | aaGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACcgc‑g > 2:814372/1‑150 (MQ=255) agagCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCgc‑gc > 1:608558/1‑150 (MQ=255) gCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTag < 2:360777/150‑1 (MQ=255) cgcgAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCAt < 1:297893/150‑1 (MQ=255) gcgAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCAtt > 2:470070/1‑150 (MQ=255) gcgAAGAGAACATCACCTTTTCAATGCACAAAGAGCTGCGGGTTTGCTTTCTTTGCTTACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGCTGCTAGC‑GCGGTCAtt < 2:336907/150‑1 (MQ=255) agagAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTc < 2:608558/150‑1 (MQ=255) tCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGAAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAgg > 1:1097239/1‑145 (MQ=255) tCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGAAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAgg < 2:1097239/145‑1 (MQ=255) cACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGtcctcc < 2:572554/150‑1 (MQ=255) aTGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAAcc < 1:470070/150‑1 (MQ=255) cccAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTACCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGa < 1:977382/150‑1 (MQ=255) aaaGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGActc > 2:514745/1‑150 (MQ=255) ctcgctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGTTCCTGCAATGACCGCTGCTAGC‑GCGGt > 2:998587/1‑100 (MQ=255) ctcgctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGTTCCTGCAATGACCGCTGCTAGC‑GCGGt < 1:998587/100‑1 (MQ=255) ctcgctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGc < 1:814372/150‑1 (MQ=255) ctcgctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGAACGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGc > 2:36432/1‑150 (MQ=255) tcgctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGca < 1:959222/150‑1 (MQ=255) tGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGAt < 1:501438/139‑1 (MQ=255) tGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGAt > 2:501438/1‑139 (MQ=255) aCGGGCAGTACCGCCGCCAATGCAGGGGCTGCTCTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGatat > 2:733646/1‑150 (MQ=255) gggCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCg > 2:117894/1‑150 (MQ=255) gCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGc < 1:117894/150‑1 (MQ=255) aCCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGa < 1:830053/150‑1 (MQ=255) aCCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCACGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGa < 1:397562/150‑1 (MQ=255) aCCGCCGCCAATGCAGGGGCTGCTCTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGa < 1:733646/150‑1 (MQ=255) ccgccgCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGGAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGag > 1:35917/1‑150 (MQ=255) gccgccAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGAct > 2:98292/1‑108 (MQ=255) gccgccAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGAct < 1:98292/108‑1 (MQ=255) gCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAg > 1:719828/1‑150 (MQ=255) accgtGCGCGACTCAAGCCAGACCCTGGAATGGCCGC‑GCTATCATCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGtttt < 2:1163505/146‑1 (MQ=255) ccTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGGAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTg < 2:35917/150‑1 (MQ=255) aaGACCGCTCCTGCAATGCCCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTGCGCCCAACCAGCt > 2:186170/1‑150 (MQ=255) aaGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCATTGCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTGCGCCCAACCAGCt > 2:533887/1‑150 (MQ=255) gCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTGCGCCCAACCAGCTgagatc > 1:633532/1‑145 (MQ=255) cTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTGCGCCCAACCAGCTGTTGccc < 1:514745/150‑1 (MQ=255) cAATGACCGCTGCTAGC‑GCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTGCGCCCAACCAGCTGt > 2:1044406/1‑139 (MQ=255) cAATGACCGCTGCTAGC‑GCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTGCGCCCAACCAGCTGt < 1:1044406/139‑1 (MQ=255) ccGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTGCGCCCAACCAGCTGTTGCCCGACGTCATGGGc > 1:716014/1‑150 (MQ=255) | AAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTGCGCCCAACCAGCTGTTGCCCGACGTCATGGGC > NC_002947/714864‑715158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |