| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,850,603:1 | +A | 7.7% | coding (41/474 nt) | PP_3398 → | curli fiber surface‑exposed nucleator CsgB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,850,603 | 1 | . | A | 7.7% | 195.9 / 11.1 | 52 | coding (41/474 nt) | PP_3398 | curli fiber surface‑exposed nucleator CsgB |
| Reads supporting (aligned to +/- strand): ref base . (24/24); new base A (2/2); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01 | |||||||||||
ATAGCACCACGATCATCCCGACTGCGGCACCGACGGCGACGATCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAGCAACAAGGGTTAGGCAACCGCGCC > NC_002947/3850456‑3850751 | aTAGCACCACGATCATCCCGACTGCGGCACCGACGGCGACGATCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑gg < 2:1165708/150‑1 (MQ=255)aTAGCACCACGATCATCCCGACTGCGGCACCGACGGCGACGAGCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑gg < 1:198353/150‑1 (MQ=255) ctaacccttgttgctcgatcaccgcagcctggccggcaccggccggcagcaggcggatgatcactggttcaccgaggtcgttgccgggtgcaaggtcggcattgtccatcaggtcctctgcccagcagGCCTGGCCCTGGC‑GGGCCAGGc > 1:582173‑M1/129‑150 (MQ=255) tcCCGACTGCGGCACCGACGGCGACGATCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑gg < 1:704517/135‑1 (MQ=255) tcCCGACTGCGGCACCGACGGCGACGATCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑gg > 2:704517/1‑135 (MQ=255) tcCCGACTGCGGCACCGACGGCGACGATCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTggg > 1:942438/1‑150 (MQ=255) ccGACTGCGGCACCGACGGCGACGATCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGTGCa < 1:102076/150‑1 (MQ=255) ccGACTGCGGCACCGACGGCGACGATCACCACCAGCCAAGGCACCACGCGAACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCa > 1:1018761/1‑150 (MQ=255) gACTGCGGCACGTGCGGCGACGATCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCaga < 2:520024/150‑1 (MQ=255) ggCACCGACGGCGACGATCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCt < 1:127943/150‑1 (MQ=255) cACCGACGGCGACGATCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGa < 2:655087/150‑1 (MQ=255) gACGGCGACGATCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTCGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGa < 2:808107/150‑1 (MQ=255) gCGACGATCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGGAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAAt > 1:1033831/1‑150 (MQ=255) cgacgaTCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATg < 2:578557/150‑1 (MQ=255) gaTCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGa < 2:942438/150‑1 (MQ=255) gaTCACCACCAGCAAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGa < 2:278243/150‑1 (MQ=255) accaccAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCtt < 2:241081/150‑1 (MQ=255) caccaGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGc > 2:1020292/1‑150 (MQ=255) accaGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCa < 2:468665/150‑1 (MQ=255) gCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACg > 1:755384/1‑150 (MQ=255) ccacGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGAcc > 2:638210/1‑150 (MQ=255) ccacGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAAGGCCGACCTTGCACCCGGCAACGAcc > 1:907710/1‑150 (MQ=255) cacGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCt > 2:234222/1‑150 (MQ=255) cacGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCt > 2:1021610/1‑150 (MQ=255) acGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGAc < 1:1123728/111‑1 (MQ=255) acGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGAc > 2:1123728/1‑111 (MQ=255) tCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAg < 2:1018761/150‑1 (MQ=255) gTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGatcaccgca > 2:654845/1‑145 (MQ=255) aGCCGGAGGCTTCGATGGAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTgccgg < 2:1033831/150‑1 (MQ=255) gacGGAGGCTTCGATGAACCGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCAGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTgccggc > 2:849637/3‑150 (MQ=255) gAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGt > 2:150488/1‑150 (MQ=255) gCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTCATGGCCAACGCCGACCTTGCACCCGGCAACGACCTCGGTGAGCTAGTGATCATCCGCCTGCTGCCGGCCGGTTcc < 1:352245/150‑1 (MQ=255) cTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCCGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCg < 1:727827/150‑1 (MQ=255) aTGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAg > 1:742328/1‑150 (MQ=255) tGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAgg < 2:917494/150‑1 (MQ=255) gAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGCACCGGccggca < 1:132536/150‑5 (MQ=255) aaCGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTg > 1:123574/1‑150 (MQ=255) cTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGa < 1:251745/150‑1 (MQ=255) tCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAg > 1:292246/1‑150 (MQ=255) ctctgcccagcagGCCTGGCCC‑GCCAGGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGt > 1:1204537‑M1/14‑93 (MQ=255) ctcggcccagcagGCCTGGCCC‑GCCAGGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGt < 2:1204537‑M1/80‑1 (MQ=255) ccTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGCTGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAGca > 1:192514/1‑150 (MQ=255) ccTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAGca > 2:94952/1‑150 (MQ=255) ccTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAGca > 1:443512/1‑150 (MQ=255) cTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAGcaa > 2:1197266/1‑150 (MQ=255) cccagcagGCCTGGCCC‑GCCAGGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGat > 2:724762‑M1/9‑99 (MQ=255) cccagcagGCCTGGCCC‑GCCAGGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGat < 1:724762‑M1/91‑1 (MQ=255) tGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAGCAACAAg < 2:907710/150‑1 (MQ=255) ctctGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAGCAACAAggg > 2:120410/1‑150 (MQ=255) tctGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCctgc < 2:699308/105‑1 (MQ=255) tctGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCctgc > 1:699308/1‑105 (MQ=255) TGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAGCAACAAgg < 2:353847‑M1/142‑1 (MQ=255) TGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAGCAACAAgg > 1:353847‑M1/1‑142 (MQ=255) GCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAGCAACAAGGGTTAGGCAAc < 2:666468‑M1/150‑1 (MQ=255) CCTGGC‑GGGCCAGGCCTGCTGGGCGGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAGCAACAAGGGTTAGGCAACcg < 1:345159‑M1/150‑1 (MQ=255) CTGGC‑TGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAGCAACAAGGGTTAGGCAACcgc < 2:902279‑M1/150‑1 (MQ=255) gc‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAGCAACAAGGGTTAGGCAACCGcgcc < 1:862625/150‑1 (MQ=255) | ATAGCACCACGATCATCCCGACTGCGGCACCGACGGCGACGATCACCACCAGCCAAGGCACCACGCGCACCGTCAACGTAGTCGTGCAGTAAAAGCCGGAGGCTTCGATGAAACGGCTGTACTCCCTGTTGCTCTGCCTGGCCCTGGC‑GGGCCAGGCCTGCTGGGCAGAGGACCTGATGGACAATGCCGACCTTGCACCCGGCAACGACCTCGGTGAACCAGTGATCATCCGCCTGCTGCCGGCCGGTGCCGGCCAGGCTGCGGTGATCGAGCAACAAGGGTTAGGCAACCGCGCC > NC_002947/3850456‑3850751 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |