| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 68,340 | G→T | 15.5% | intergenic (+28/+147) | PP_0057 → / ← PP_0058 | major facilitator family transporter/1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 68,340 | 0 | G | T | 15.5% | 149.7 / 12.9 | 57 | intergenic (+28/+147) | PP_0057/PP_0058 | major facilitator family transporter/1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (25/23); new base T (4/5); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TGGTCTATACCGGGGATATCTTCTACGGGCTGTGGTACCCGGTGCTGGTGACCGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGCATTTAATCCAAAAACTGCACACAAGTTATCCACAGAAGGTC > NC_002947/68191‑68488 | tggtCTATACCGGGGATATCTTCTACGGGCTGTGGTACCCGGTGCTGGTGACCGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTg < 2:1125789/150‑1 (MQ=255) atCTATACCGGGGATATCTTCTACGGGCTGTGGTACCCGGTGCTGGTGACCGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTa > 2:828972/2‑150 (MQ=255) taCCGGGGATATCTTCTACGGGCTGTGGTACCCGGTGCTGGTGACCGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGc < 1:1077309/150‑1 (MQ=255) ccGGGGATATCTTCTACGGGCTGTGGTACCCGGTGCTGGTGACCGGGATCAGCCTGGTGGTGGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCg < 1:452123/150‑1 (MQ=255) cGGGGATATCTTCTACGGGCTGTGGTACCCGGTGCTGGTGACCGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCgg < 1:354695/150‑1 (MQ=255) tcttctACGGGCTGTGGTACCCGGTGCTGGTGACCGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTa < 1:849383/150‑1 (MQ=255) cttctACGGGCTGTGGTACCCGGTGCTGGTGACCGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTTTTACAGCCGGTTTTTTTAt < 2:1032361/150‑1 (MQ=255) ttctACGGGCTGTGGTACCCGGTGCTGGTGACCGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATg > 1:1068358/1‑150 (MQ=255) gCTGTGGTACCCGGTGCTGGTGACCGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCaaa‑a > 2:515027/1‑150 (MQ=255) tACCCGGTGCTGGTGACCGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTa > 1:714595/1‑150 (MQ=255) ggTGCTGGTGACCGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCAc < 1:515027/150‑1 (MQ=255) cGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTTTTACAGCCGGttttt > 2:967100/1‑113 (MQ=39) cGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTTTTACAGCCGGttttt < 1:967100/113‑1 (MQ=39) cGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGaaa > 2:270476/1‑150 (MQ=255) cGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGaaa > 2:1125748/1‑150 (MQ=255) aGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCAATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCa < 2:94802/150‑1 (MQ=255) ggtTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGa < 1:828972/150‑1 (MQ=255) ttGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGaaa < 2:1068358/150‑1 (MQ=255) gATCTTCTGTCTGAGAGAAACCAAGGATTTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAAc > 1:1032544/1‑150 (MQ=255) gATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAAc < 2:714595/150‑1 (MQ=255) tctgAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAAtt > 1:963587/1‑150 (MQ=255) tgAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCa > 2:482523/1‑150 (MQ=255) gagagaAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCAt > 2:114681/1‑150 (MQ=255) agagaAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATaa > 2:951177/1‑136 (MQ=255) agagaAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATaa < 1:951177/136‑1 (MQ=255) gagaAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTc < 1:925723/146‑1 (MQ=255) gagaAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTc > 2:925723/1‑146 (MQ=255) agaAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACCTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATaca < 1:733796/150‑1 (MQ=255) agaAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACCTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATaca > 2:733796/1‑150 (MQ=255) atCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGc > 2:1179956/1‑150 (MQ=255) atCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGc > 2:1179955/1‑150 (MQ=255) atCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATG‑CTCAAATGAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGc < 1:927956/150‑1 (MQ=255) tCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCaa < 2:1080197/147‑1 (MQ=255) tCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCaa > 1:1080197/1‑147 (MQ=255) aCAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGCAttt > 2:340220/1‑150 (MQ=255) aaaGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGCATTTaa < 1:114681/150‑1 (MQ=255) aaaGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGCATTTaa > 2:176018/1‑150 (MQ=255) aaGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCAAAAAAAAAAAAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGCATTTAAt < 2:997223/150‑1 (MQ=255) tCTGATGTCCACATGCCATAAAAAAACCGGCTTTTACAGCCGGttt < 1:810250/46‑1 (MQ=14) tCTGATGTCCACATGCCATAAAAAAACCGGCTTTTACAGCCGGttt > 2:810250/1‑46 (MQ=14) tGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGCATTTAATCCaa > 1:212183/1‑149 (MQ=255) tGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGCATTTAATCCaa < 2:212183/149‑1 (MQ=255) aTGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAg > 2:414533/1‑135 (MQ=255) aTGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAg < 1:414533/135‑1 (MQ=255) acaTGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGCATTTAATCCAAAAACTGcac > 1:892648/1‑150 (MQ=255) gCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGCATTTAATCCAAAAACTGCACACaa < 1:1125748/150‑1 (MQ=255) gcATAAAAAAACCGGCTTTTACAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGatat < 2:596979/114‑1 (MQ=255) gcATAAAAAAACCGGCTTTTACAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGatat > 1:596979/2‑115 (MQ=255) aTAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTc > 1:900368/1‑119 (MQ=255) aTAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTc < 2:900368/119‑1 (MQ=255) aaaaaaCCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGCATTTAATCCAAAAACTGCACACAAGTTATc < 2:1032544/150‑1 (MQ=255) aaaaaCCGGCTTTTACAGCCGGTTTTTTTATGGCTCAAA‑AAAACTTATGCATGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAAtt > 2:549764/1‑94 (MQ=25) aaaaaCCGGCTTTTACAGCCGGTTTTTTTATGGCTCAAA‑AAAACTTATGCATGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAAtt < 1:549764/94‑1 (MQ=37) aaCCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTc > 2:147436/1‑92 (MQ=255) aaCCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTc < 1:147436/92‑1 (MQ=255) aCCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGCATTTAATCCAAAAACTGCACACAAGTTATCCACAg < 1:340220/150‑1 (MQ=255) tGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCCAATTCATACACTTAGATATCAATTCAAGCATTTAATCCAAAAACTGCACACAAGTTATCCACAGAAGGTc > 2:342940/1‑150 (MQ=255) tGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGCATTTAATCCAAAAACTGCACACAAGTTATCCACAGAAGGTc > 2:1141377/1‑150 (MQ=255) | TGGTCTATACCGGGGATATCTTCTACGGGCTGTGGTACCCGGTGCTGGTGACCGGGATCAGCCTGGTGGTTGGGATCTTCTGTCTGAGAGAAACCAAGGATGTAGATATCGACAAAGTCTGATGTCCACATGCCATAAAAAAACCGGCTGTAAAAGCCGGTTTTTTTATGCCTCAAA‑AAAACTTATGCACGATTTTCAGAAAAAATTCATACAAAGAAATAACTAGCAAATTCATACACTTAGATATCAATTCAAGCATTTAATCCAAAAACTGCACACAAGTTATCCACAGAAGGTC > NC_002947/68191‑68488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |