Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,660,295	C→G	8.7%	intergenic (+97/‑132)	metK → / → ligB	methionine adenosyltransferase/DNA ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,660,295	0	C	G	8.7%	141.5 / 3.4	46	intergenic (+97/‑132)	metK/ligB	methionine adenosyltransferase/DNA ligase B
Reads supporting (aligned to +/- strand):  ref base C (21/21);  new base G (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTAAGCGCTTCCCCGCCATGCAAGGATGCCAGGCCATGCCGTAC  >  NC_002947/5660146‑5660435                                                                                                                                                      |                                                                                                                                             ccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGcgcc                                                                                                                                              <  1:558401/150‑1 (MQ=255)   tGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGcgccgc                                                                                                                                            <  2:602408/150‑1 (MQ=255)           cACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCGGCGCTCgatc                                                                                                                                    <  1:544380/150‑4 (MQ=255)            accgaccgCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCCGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTAAGATCGAGCGCCGCGCTCGttt                                                                                                                                    >  1:339506/1‑149 (MQ=255)            accgaccgCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTc                                                                                                                                   >  1:729956/1‑150 (MQ=255)            accgaccgCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTAAGATCGAGCGCCGCGCTCGttt                                                                                                                                    <  2:339506/149‑1 (MQ=255)              cgaccgCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCGGCGCTCgatctga                                                                                                                                 <  1:106497/150‑7 (MQ=255)                  cgcgCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTc                                                                                                                             >  1:301307/1‑150 (MQ=255)                        cAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGtctc                                                                                                                       <  2:660942/150‑1 (MQ=255)                                    gACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGAc                                                                                                           <  1:1108871/150‑1 (MQ=255)                                      cGCTGCCGGCCTGTAAGCTTCCTGCAGCCCCAGACGCAAGCCTCTGCCGAGCGATCGGCAGGGGCTTTTTTCCGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGa                                                                                                         <  1:870069/150‑1 (MQ=255)                                            cGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCa                                                                                                   <  2:840016/150‑1 (MQ=255)                                              gCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGc                                                                                                 >  1:1061620/1‑150 (MQ=255)                                                 tGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTg                                                                                                                                >  1:1061458/1‑116 (MQ=255)                                                 tGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTg                                                                                                                                <  2:1061458/116‑1 (MQ=255)                                                   tAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGaaa                                                                                             <  2:562162/149‑1 (MQ=255)                                                   tAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGaaa                                                                                             >  1:562162/1‑149 (MQ=255)                                                   tAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAat                                                                                            <  2:729956/150‑1 (MQ=255)                                                        ttCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCAc                                                                                       >  1:842092/1‑150 (MQ=255)                                                        ttCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCAc                                                                                       <  2:842092/150‑1 (MQ=255)                                                         tCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATCTCAcc                                                                                      <  2:301307/150‑1 (MQ=255)                                                         tCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCAcc                                                                                      <  1:18102/150‑1 (MQ=255)                                                            tGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGa                                                                                   <  2:626489/150‑1 (MQ=255)                                                             gCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCg                                                                                                                      <  2:915555/114‑1 (MQ=255)                                                             gCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCg                                                                                                                      >  1:915555/1‑114 (MQ=255)                                                              cAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACa                                                                                 >  2:479455/1‑150 (MQ=255)                                                                         gAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTgcacgcac                                                                      >  1:998451/1‑150 (MQ=255)                                                                         gAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTgcacgcac                                                                      >  1:1145970/1‑150 (MQ=255)                                                                                   gCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGcccgcccc                                                            <  1:413268/150‑1 (MQ=255)                                                                                          gATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCAccc                                                     >  1:431378/1‑150 (MQ=255)                                                                                            tCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGc                                                   <  2:652236/150‑1 (MQ=255)                                                                                                   gggCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCt                                            >  2:109306/1‑150 (MQ=255)                                                                                                    ggCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTa                                           >  1:959032/1‑150 (MQ=255)                                                                                                      cTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTAAg                                         >  1:164354/1‑150 (MQ=255)                                                                                                       ttttttGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTAAgc                                        >  1:659786/1‑150 (MQ=255)                                                                                                               gCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTAAGCGCTTcccc                                >  1:883598/1‑150 (MQ=255)                                                                                                               gCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTAAGCGCTTcccc                                <  1:479455/150‑1 (MQ=255)                                                                                                                      aCGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTAAGCGCTTCCCCGCCATGc                         <  1:109306/150‑1 (MQ=255)                                                                                                                              ttCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTAAGCGCTTCCCCGCCATGCAAGGATGc                 <  1:1078707/150‑1 (MQ=255)                                                                                                                                cATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTAAGCGCTTCCCCGCCATGCAAGGATGCCa               <  2:959032/150‑1 (MQ=255)                                                                                                                                 aTCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCgcccgccc                                                             <  2:609033/103‑1 (MQ=255)                                                                                                                                 aTCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCgcccgccc                                                             >  1:609033/1‑103 (MQ=255)                                                                                                                                 aTCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTAAGCGCTTCCCCGCCATGCAAGGATGCCAg              >  1:297596/1‑150 (MQ=255)                                                                                                                                     cccGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTAAGCGCTTCCCCGCCATGCAAGGATGCCAGGCCa          >  2:813434/1‑150 (MQ=255)                                                                                                                                       cGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACCACTGCACGCCCCGCCCGCCCCGCCACCCGCCTACGACAAGAGCATCCACGCCCGGCAAGGAAGCCAGGCCATg        <  2:980096/150‑1 (MQ=255)                                                                                                                                           gaaacGAGCGCGGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATTACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTAAGCGCTTCCCCGCCATGCAAGGATGCCAGGCCATGCCGt    >  2:421800/5‑150 (MQ=255)                                                                                                                                            aaacGAGCGCGGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTAAGCGCTTCCCCGCCATGCAAGGATGCCAGGCCATGCCGTa   >  1:299060/4‑150 (MQ=255)                                                                                                                                             aTCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTAACCGCTTCCCCGCCATGCAAGGATGCCAGGCCATGCCGTAc  >  1:969066/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                             CCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCCGCCTACGCTAAGCGCTTCCCCGCCATGCAAGGATGCCAGGCCATGCCGTAC  >  NC_002947/5660146‑5660435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑