Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,679,489 A→G 11.3% M355V (ATG→GTG)  bioA → adenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,679,4890AG11.3% 138.8 / 7.7 53M355V (ATG→GTG) bioAadenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA
Reads supporting (aligned to +/- strand):  ref base A (25/22);  new base G (3/3);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

GTTCTATGACGATTACCCGACACTGCGCGCATTCCTGCACTCGCACAGCTACACCGGCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGCCTACCCCTGGCAGGAGCGCCGTGGCCTGAAGGTGTTC  >  NC_002947/5679341‑5679635
                                                                                                                                                    |                                                                                                                                                  
gTTCTATGACGATTACCCGACACTGCGCGCATTCCTGCACTCGCACAGCTACACCGGCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACACGGCCCTGGCCACGCGCAt                                                                                                                                                   <  1:1189526/150‑1 (MQ=255)
   cTATGACGATTACCCGACACTGCGCGCATTCCTGCACTCGCACAGCTACACCGGCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGc                                                                                                                                                <  1:705124/150‑1 (MQ=255)
          gATTACCCGACACTGCGCGCATTCCTGCACTCGCACAGCTACACCGGCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGcc                                                                                                                                         >  1:389194/1‑150 (MQ=255)
                ccGACACTGCGCGCATTCCTGCACTCGCACAGCTACACCGGCAACCCGCTGGCGTGCGCTGCAGTGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGca                                                                                                                                   >  1:1138259/1‑150 (MQ=255)
                     acTGCGCGCATTCCTGCACTCGCACAGCTACACCGGCAACCCGCTGGCGTGCGCTGCCGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACAttt                                                                                                                              <  2:342613/150‑1 (MQ=255)
                          gcgcATTCCTGCACTCGCACAGCTACACCGGCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCg                                                                                                                         >  2:303916/1‑150 (MQ=255)
                                      aCTCGCACAGCTACACCGGCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCGCATg                                                                                                             >  2:477533/1‑150 (MQ=255)
                                          gCACAGCTACACCGGCAACCCGCTGGCGTGCGCTGACGCGCTGGCGACCCTGGCTCCATTCGAGCAGGACAAAGTTTTCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGc                                                                                                         >  1:1053147/1‑150 (MQ=255)
                                          gCACAGCTAAACCGGCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGc                                                                                                         >  2:1079665/1‑150 (MQ=255)
                                            acaGCTACACCGGCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGAGCTAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCg                                                                                                       >  1:81261/1‑150 (MQ=255)
                                            acaGCTACACCGGCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAACAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTgg                                                                                                                            <  2:631969/129‑1 (MQ=255)
                                            acaGCTACACCGGCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAACAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTgg                                                                                                                            >  1:631969/1‑129 (MQ=255)
                                                  acacCTGCAACCCGCTGGCGTGCGCTGCAGCGCTGGTGACCCTGGACATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATAc                                                                                                 <  2:1053147/150‑1 (MQ=255)
                                                    acCGGCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGc                                                                                               <  2:389194/150‑1 (MQ=255)
                                                     ccGGCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGcc                                                                                              >  2:61680/1‑150 (MQ=255)
                                                      cGGCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCa                                                                                             >  2:119456/1‑150 (MQ=255)
                                                       ggCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGc                                                                                                                           >  1:235430/1‑119 (MQ=255)
                                                       ggCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGc                                                                                                                           <  2:235430/119‑1 (MQ=255)
                                                           aCCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTg                                                                                        >  1:224256/1‑150 (MQ=255)
                                                           aCCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTg                                                                                        <  2:673859/150‑1 (MQ=255)
                                                             ccGCTGGCGTGCGCTCCCGCGCTGGCGACCCTGGATAGCTTCGAGCAGGACAACGTGGTCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGc                                                                                      >  2:12622/1‑150 (MQ=255)
                                                                cTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATg                                                                                   <  1:61680/150‑1 (MQ=255)
                                                                   gCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGcc                                                                                <  2:224256/150‑1 (MQ=255)
                                                                     gTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGccag                                                                              <  1:357635/150‑3 (MQ=255)
                                                                                              gATATCTTCGAGCAGGACAACGTGATCGATCCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAag                                                     <  1:306688/150‑1 (MQ=255)
                                                                                               atatCTTCGATCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCGTGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCATACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAagg                                                    <  1:1152872/150‑1 (MQ=255)
                                                                                                            ggACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGaaa                                                                   >  2:313669/1‑122 (MQ=255)
                                                                                                            ggACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGaaa                                                                   <  1:313669/122‑1 (MQ=255)
                                                                                                            ggACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGc                                       >  2:233745/1‑150 (MQ=255)
                                                                                                             gACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGc                                                                                               <  2:103789/93‑1 (MQ=255)
                                                                                                             gACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGc                                                                                               >  1:103789/1‑93 (MQ=255)
                                                                                                                    tGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTgg                                                                            >  1:789634/1‑105 (MQ=255)
                                                                                                                    tGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTgg                                                                            <  2:789634/105‑1 (MQ=255)
                                                                                                                    tGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGCCTACCCCt                               <  1:303916/150‑1 (MQ=255)
                                                                                                                     gATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGtt                                                              >  2:974621/1‑118 (MQ=255)
                                                                                                                     gATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGtt                                                              <  1:974621/118‑1 (MQ=255)
                                                                                                                           ggCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGCCTACCCCTGGCAGGa                        <  2:604134/150‑1 (MQ=255)
                                                                                                                           ggCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGCCTACCCCTGGCAGGa                        >  1:490085/1‑150 (MQ=255)
                                                                                                                               aacaaGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGCCTACCCCTGGCAGGAgcgc                    >  2:562516/1‑150 (MQ=255)
                                                                                                                                 caaGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGCCTACCCCTGGCAGGAgcgcgg                  >  1:973470/1‑148 (MQ=255)
                                                                                                                                     gCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATc                                                                       <  2:177725/93‑1 (MQ=255)
                                                                                                                                     gCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATc                                                                       >  1:177725/1‑93 (MQ=255)
                                                                                                                                       tgtGGCCATGCGCGTGGCCAGGGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGCCTACCCCTGGCAGGAGCGCCGTGGCCt            >  2:28894/3‑150 (MQ=255)
                                                                                                                                       tgtGGCCATGCGCGTGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGc                                                                                 <  1:1048412/79‑1 (MQ=255)
                                                                                                                                       tgtGGCCATGCGCGTGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGc                                                                                 >  2:1048412/3‑81 (MQ=255)
                                                                                                                                       tgtGGCCATGCGCGTGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGaa                                                                    >  1:529888/3‑94 (MQ=255)
                                                                                                                                       tgtGGCCATGCGCGTGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGaa                                                                    <  2:529888/92‑1 (MQ=255)
                                                                                                                                        cTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGCCTACCCCTGGCAGGAGCGCCGTGGCCTg           >  2:252442/1‑150 (MQ=255)
                                                                                                                                          ggCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGCCTACCCCTGGCAGGAGCGCCGTGGCCTGaa         >  1:370039/1‑150 (MQ=255)
                                                                                                                                          ggCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGCCTACCCCTGGCAGGAGCGCCGTGGCCTGaa         <  2:1138259/150‑1 (MQ=255)
                                                                                                                                             cACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGCCTACCCCTGGCAGGAGCGCCTTGGCCTGAAGgt      >  2:375096/1‑150 (MQ=255)
                                                                                                                                                 cgcATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGCCTACCCCTGGCAGGAGCGCCGTGGCCTGAAGGTGTTc  <  1:233745/150‑1 (MQ=255)
                                                                                                                                                 cgcATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGCCTACCCCTGGCAGGAGCGCCGTGGCCTGAAGGTGTTc  <  1:1079665/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTTCTATGACGATTACCCGACACTGCGCGCATTCCTGCACTCGCACAGCTACACCGGCAACCCGCTGGCGTGCGCTGCAGCGCTGGCGACCCTGGATATCTTCGAGCAGGACAACGTGATCGAGGCCAACAAGGCCCTGGCCACGCGCATGGCCACAGCCACTGCACATTTGGCCGATCATGCTCATGTTGCCGAAATACGCCAGACTGGCATGGCCCTGGCCATCGAAATGGTTCAAGACAAGGCAGGCAAGCTTGCCTACCCCTGGCAGGAGCGCCGTGGCCTGAAGGTGTTC  >  NC_002947/5679341‑5679635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑