Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,685,958 G→C 8.5% P331A (CCC→GCC) ‡ PP_4988 ← signal transduction histidine kinase CheA/CheY‑like receiver

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,685,9580GC 8.5% 135.2 / 4.3 47P331A (CCC→GCC) ‡PP_4988signal transduction histidine kinase CheA/CheY‑like receiver
Reads supporting (aligned to +/- strand):  ref base G (24/19);  new base C (2/2);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

GCTCATCAATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTTC  >  NC_002947/5685817‑5686102
                                                                                                                                             |                                                                                                                                                
gCTCATCAATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAgcc                                                                                                                                          >  1:1081709/1‑148 (MQ=255)
    atcaATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTg                                                                                                                                      >  1:675127/1‑150 (MQ=255)
    atcaATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTg                                                                                                                                      >  1:42513/1‑150 (MQ=255)
       aaTCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCg                                                                                                                                   >  1:182072/1‑150 (MQ=255)
        aTCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAGCCCTTGCCgg                                                                                                                                  <  1:896465/150‑1 (MQ=255)
         tCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAGCCCTTGCCggg                                                                                                                                 <  2:1081709/150‑1 (MQ=255)
                ctctTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTc                                                                                                                          >  2:890135/1‑150 (MQ=255)
                   ttCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGa                                                                                                                       <  1:463791/150‑1 (MQ=255)
                      ctccAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGctgttgc                                                                                                                         >  2:545724/1‑139 (MQ=255)
                      ctccAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGctgttgc                                                                                                                         <  1:545724/145‑7 (MQ=255)
                         cAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                 >  1:675078/1‑150 (MQ=255)
                           ggTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCAGACAAACAGCTCGGCCGCGGCCGGCAGCATCAACATGGCCAATGACCCGCGGATCTGTTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTACCGGGGCGAGTCGGACTCCAggg                                                                                                               >  2:488791/1‑149 (MQ=255)
                               ctgcAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATc                                                                                                           >  1:934036/1‑150 (MQ=255)
                                    accaacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGa                                                                                                      <  2:675127/150‑1 (MQ=255)
                                     ccaacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCGGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                 >  1:1152040/1‑138 (MQ=255)
                                     ccaacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                 <  2:1152040/138‑1 (MQ=255)
                                     ccaacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGAt                                                                                                     >  2:925081/1‑150 (MQ=255)
                                      caacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATc                                                                                                    >  2:1053894/1‑150 (MQ=255)
                                       aacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCa                                                                                                   <  1:1053894/150‑1 (MQ=255)
                                                 aTAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTGGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGc                                                                                         >  1:378717/1‑150 (MQ=255)
                                                     cTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCctgc                                                                                     >  2:674296/1‑150 (MQ=255)
                                                      ttGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCctgct                                                                                    <  2:525922/150‑1 (MQ=255)
                                                            aGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAaca                                                                              >  2:769787/1‑150 (MQ=255)
                                                             gCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAacac                                                                             <  2:934036/150‑1 (MQ=255)
                                                               cGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGt                                                                           >  2:138452/1‑150 (MQ=255)
                                                                gACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGtt                                                                          >  2:1154279/1‑150 (MQ=255)
                                                                     aCAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTaa                                                                                                             <  2:86136/110‑1 (MQ=255)
                                                                     aCAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGATGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTaa                                                                                                             >  1:86136/1‑110 (MQ=255)
                                                                         cTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGattc                                                                 <  1:925081/150‑1 (MQ=255)
                                                                         cTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGattc                                                                 >  2:34089/1‑150 (MQ=255)
                                                                               cACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAg                                                           >  1:1059903/1‑150 (MQ=255)
                                                                                      ggcAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGc                                                    >  1:251650/1‑150 (MQ=255)
                                                                                          gcaTCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGc                                                <  1:1154279/150‑1 (MQ=255)
                                                                                               aaCATGGCCAAGGCCCCGCGGGTCTGCTGCAACAGCCCCGGCAAGCGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTAtc                                           <  2:378717/150‑1 (MQ=255)
                                                                                                aCATGGCCAATGCGCCGCGGATCTGCTGCAACAGCCCCGGCAAGTGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTAtct                                          >  2:239944/1‑150 (MQ=255)
                                                                                                        aaTGACCCGCGGATCTGCTGCAACAGACCCGGCAAGGGCTGCAGGCGTTGCCGGTGCCAGTCGAACTCCAGGTAATCGCCGATCAGATCCTGGGCCTGCGGCAACACGTTCAGCGCTTCATTCAGCACCAGCTGGCGTATCTCGGCCAgg                                  >  2:410218/1‑150 (MQ=255)
                                                                                                           gCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGAAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAaca                                                                              >  1:924180/1‑103 (MQ=255)
                                                                                                           gCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGAAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAaca                                                                              <  2:924180/103‑1 (MQ=255)
                                                                                                                gcgGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGcc                          <  2:984134/150‑1 (MQ=255)
                                                                                                                     tCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGTCCAGGTCCGAGCCCGGCa                     <  1:674296/150‑1 (MQ=255)
                                                                                                                              aCAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCtt            <  1:138452/150‑1 (MQ=255)
                                                                                                                               tggCCCCGGCAACGCCTGCAGCCCTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTg           >  1:541160/3‑150 (MQ=255)
                                                                                                                                aGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTGCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTgg          <  1:239944/150‑1 (MQ=255)
                                                                                                                                     cGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGtt     <  2:675078/150‑1 (MQ=255)
                                                                                                                                      ggCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGttt    <  2:42513/150‑1 (MQ=255)
                                                                                                                                       gCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGtttt   >  2:116647/1‑150 (MQ=255)
                                                                                                                                        cAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTtc  <  1:890135/150‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                
GCTCATCAATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTTC  >  NC_002947/5685817‑5686102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑