| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,698,213 | G→T | 11.1% | R264L (CGT→CTT) | hslU → | protease HslVU ATPase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,698,213 | 0 | G | T | 11.1% | 125.6 / 6.5 | 45 | R264L (CGT→CTT) | hslU | protease HslVU ATPase subunit |
| Reads supporting (aligned to +/- strand): ref base G (23/17); new base T (2/3); total (25/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01 | |||||||||||
AAGCTGAAGGTGAAAGAAGCGCTGAAGATGGTTCGCGATGAAGAAGCGAGCCGCCTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGTCAAGACCGACCACATTCTGTTCATTGCCTCGGGTGCATTCCACCTGAG > NC_002947/5698065‑5698360 | aaGCTGAAGGTGAAAGAAGCGCTGAAGATGGTTCGCGATGAAGAAGCGAGCCGCCTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGt < 1:407400/150‑1 (MQ=255) cTGAAGGTGAAAGAAGCGCTGAAGATGGTTCGCGATGAAGAAGCGAGCCGCCTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGc > 1:951967/1‑150 (MQ=255) tGAAGGTGAAAGAAGCGCTGAAGATGGTTCGCGATGAAGAAGCGAGCCGCCTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCa > 2:388520/1‑150 (MQ=255) aaaGAAGCGCTGAAGATGGTTCGCGATGAAGAAGCGAGCCGCCTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAGCAGCACGGTATCGTGTTCATCGACGAAATCGCCAACGTTGCCACGCTTGGCAACCTTgtc < 1:218281/150‑3 (MQ=255) aaaGAAGCGCTGAAGATGGTTCGCGATGAAGAAGCGAGCCGCCTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCCACGTTggc < 2:951967/150‑1 (MQ=255) aaaGAAGCGCTGAAGATGGTTCGCGATGAAGAAGCGAGCCGCCTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTggc > 2:248776/1‑150 (MQ=255) tCGCGATGAAGAAGCGAGCCGCCTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCACGCTTGGCAACCTTgt < 2:5367/129‑2 (MQ=255) tCGCGATGAAGAAGCGAGCCGCCTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCACGCTTGGCAACCTTgt > 1:5367/1‑128 (MQ=255) gcgATGAAGAAGCGAGCCGCCTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAg < 2:980316/150‑1 (MQ=255) tGAAGAAGCGAGCCGCCTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGt > 2:770523/1‑150 (MQ=255) gaagaaGCGAGCGGCCTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTa < 1:248776/150‑1 (MQ=255) agCGAGCCGCCTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAgcg < 2:1067066/150‑1 (MQ=255) gccgccTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGAcc < 1:468613/150‑1 (MQ=255) gccTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTccc < 1:145755/126‑1 (MQ=255) gccTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTccc > 2:145755/1‑126 (MQ=255) gAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGaa < 1:132368/150‑1 (MQ=255) tCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCa > 1:594683/1‑150 (MQ=255) gccaaggccCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTc > 2:791995/1‑150 (MQ=255) gccaaggccCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTc > 2:451987/1‑150 (MQ=255) caaggccCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCaa < 1:231511/150‑1 (MQ=255) aaggccCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGACGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAac < 1:84553/150‑1 (MQ=255) aggccCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAaca > 2:225279/1‑150 (MQ=255) ggccCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGCTGGTAGGCTGCACCGTCAacac > 2:1183176/1‑150 (MQ=255) cTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAg > 2:789530/1‑150 (MQ=255) aGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTggg < 2:1038708/150‑1 (MQ=255) aGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAgg > 1:1095181/1‑123 (MQ=255) aGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAgg < 2:1095181/123‑1 (MQ=255) aGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGt > 1:462450/1‑145 (MQ=255) aGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGt < 2:462450/145‑1 (MQ=255) gCACGTTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGTCAAgac > 2:1018422/1‑150 (MQ=255) tCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGTCAAGACCGACcaca > 1:472492/1‑150 (MQ=255) aTCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGTCAAGACCGACCACATTCTGTTc < 1:770523/150‑1 (MQ=255) gacgaAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGTCAAGACCGACCACATTCTg < 2:79343/144‑1 (MQ=255) gacgaAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGTCAAGACCGACCACATTCTg > 1:79343/1‑144 (MQ=255) acgaAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACAcc < 2:733762/110‑1 (MQ=255) acgaAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACAcc > 1:733762/1‑110 (MQ=255) aTCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGTCAAGACCGACCACATTCTGTTCATTGCCTCg > 2:525134/1‑150 (MQ=255) aacgTTGCCACGCTTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCt < 2:380619/71‑1 (MQ=38) aacgTTGCCACGCTTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCt > 1:380619/4‑74 (MQ=38) aaGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGTCAAGACCGACCACATTCTGTTCATTGCCTCGGGTGCa > 2:639186/1‑150 (MQ=255) cAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGTCAAGACCGACCACATTCTGTTCATTGCCTCGGGTGCATTCCACCt > 2:211927/1‑150 (MQ=255) cAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGTCAAGACCGACCACATTCTGTTCATTGCCTCGGGTGCATTCCACCt < 1:388520/150‑1 (MQ=255) cAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGTCAAGACCGACCACATTCTGTTCATTGCCTCGGGTGCATTCCACCt > 1:1045515/1‑150 (MQ=255) aaGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGTCAAGACCGACCACATTCTGTTCATTGCCTCGGGTGCATTCCACCTg > 2:205622/1‑150 (MQ=255) gCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGTCAAGACCGACCACATTCTGTTCATTGCCTCGGGTGCATTCCACCTGAg > 1:867932/1‑150 (MQ=255) | AAGCTGAAGGTGAAAGAAGCGCTGAAGATGGTTCGCGATGAAGAAGCGAGCCGCCTGGTCAACGAGGAAGAGCTCAAGGCCAAGGCCCTGGAAGCAGTCGAACAGCACGGTATCGTGTTCATCGACGAAATCGACAAGGTTGCCAAGCGTGGCAACGTTGGCGGCGCCGACGTGTCCCGTGAAGGCGTACAGCGCGACCTGCTGCCGCTGATCGAAGGCTGCACCGTCAACACCAAGCTGGGCATGGTCAAGACCGACCACATTCTGTTCATTGCCTCGGGTGCATTCCACCTGAG > NC_002947/5698065‑5698360 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |