| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 374,745 | C→G | 8.8% | A11G (GCC→GGC) | PP_0312 → | electron transfer flavoprotein subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 374,745 | 0 | C | G | 8.8% | 172.4 / 7.5 | 57 | A11G (GCC→GGC) | PP_0312 | electron transfer flavoprotein subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (27/25); new base G (3/2); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
GCCGCGCCCACAGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATC > NC_002947/374597‑374893 | gCCGCGCCCACAGATCAAGGACTTGGCCGAACGGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGcc < 2:22625/150‑1 (MQ=255) cgcCCACAGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGt < 1:653419/150‑1 (MQ=255) cacaGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGATAAACGTGAACCTGCGGAGGTGCACTGATGAGCCACATTATCCGCCGCGATCCACGCGCCGAGTTGAt > 2:236344/1‑150 (MQ=255) tCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCg > 2:182838/1‑150 (MQ=255) aGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTaa > 2:257626/1‑150 (MQ=255) aGGACTTGGCCGAACTGGTGGCCGACGTACTGATCGAGGAGCACGCATCCACTGTCGCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTaa > 1:453584/1‑150 (MQ=255) cTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGt < 2:693541/150‑1 (MQ=255) tGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGATCAATTGTATGCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCt < 1:328661/150‑1 (MQ=255) gCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGc > 1:319377/1‑150 (MQ=255) gCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGc < 1:7560/150‑1 (MQ=255) cGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCTCGATCCACGCGCCGAGTGGATTGCCCGTAACCGTCTGCAc < 2:453584/150‑1 (MQ=255) cGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGcc > 1:637463/1‑150 (MQ=255) gACGTGCTGATCGGCGAGGACGCATCCACTGACCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCcg < 2:319377/150‑1 (MQ=255) gACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCAcc > 2:766848/1‑138 (MQ=255) gACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCAcc < 1:766848/138‑1 (MQ=255) aCGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCTGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCcgc > 1:235854/1‑150 (MQ=255) tGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCTGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGAt < 2:235854/150‑1 (MQ=255) tGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGAt < 2:637463/150‑1 (MQ=255) gcaggaCGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCCCGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCaa < 2:80679/148‑1 (MQ=255) gaggaCGCATACACTGTCGCAACGACGCAGACCGATAAACGTGAACCCGCGGAGGTGCACTGATGAGCGACATTATCCTCCGCGATCCACGCGCCGAGTGGATAGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAAc < 2:10169/150‑1 (MQ=255) gCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCa < 1:257626/150‑1 (MQ=255) aTCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGt < 1:869165/91‑1 (MQ=255) aTCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGc > 2:582553/1‑150 (MQ=255) aTCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATCATCCGCCGCGATCCACGCGCCGAGt > 2:869165/1‑91 (MQ=255) cccAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATgggg < 1:603879/150‑1 (MQ=255) ccAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGCTGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATg < 2:808022/146‑1 (MQ=255) ccAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATg > 1:808022/1‑146 (MQ=255) ccAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGc > 1:384738/1‑150 (MQ=255) ccAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTAGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCCGGATgatgc > 2:83724/1‑146 (MQ=255) ccAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGAGGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGCTGCAGACGCAACAGACCAGCTGGATGgcgc > 2:569911/1‑150 (MQ=255) cAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGcc < 1:582553/150‑1 (MQ=255) cGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACgg > 1:949362/1‑150 (MQ=255) gCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGc < 2:23188/150‑1 (MQ=255) aaaCGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACTCGGCGCGTGGATCGcggc > 2:330337/1‑78 (MQ=37) aaaCGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACTCGGCGCGTGGATCGcggc < 1:330337/81‑4 (MQ=37) cTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGAc > 2:563883/1‑120 (MQ=255) cTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGAc < 1:563883/120‑1 (MQ=255) aaaCGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCa > 1:765877/1‑150 (MQ=255) cGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGa > 1:910478/1‑150 (MQ=255) gTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGaa > 1:310662/1‑150 (MQ=255) aCCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCca < 2:384738/150‑1 (MQ=255) tGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACAt < 2:765877/150‑1 (MQ=255) cACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGCTTGCCg > 2:125923/1‑150 (MQ=255) cACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCg > 2:479580/1‑150 (MQ=255) tgatgaGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGaa > 1:469602/1‑130 (MQ=255) tgatgaGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGaa < 2:469602/130‑1 (MQ=255) tgatgaGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAg > 1:955921/1‑150 (MQ=255) tgatgaGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAg > 1:720298/1‑150 (MQ=255) atgaGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAGCAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGc > 1:1015705/1‑150 (MQ=255) gACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTc < 2:310662/150‑1 (MQ=255) gccgcGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAAccc < 2:697469/115‑1 (MQ=255) gccgcGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAAccc > 1:697469/1‑115 (MQ=255) gcgATCCACTCGGCGCGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCtt < 2:556875/133‑1 (MQ=255) gcgATCCACTCGGCGCGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCtt > 1:556875/1‑133 (MQ=255) gcgATCCACTCGGCGCGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCa > 1:590537/1‑150 (MQ=255) cgATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATg > 1:1196645/1‑116 (MQ=255) cgATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATg < 2:1196645/116‑1 (MQ=255) cACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAgc < 2:955921/150‑1 (MQ=255) gcgcCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCa < 2:720298/150‑1 (MQ=255) gcCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATc > 1:733784/1‑150 (MQ=255) | GCCGCGCCCACAGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGATCCACGCGCCGAGTGGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGCGATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCATC > NC_002947/374597‑374893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |