Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,755,182 C→G 10.3% I80M (ATC→ATG)  glnG → two‑component system DNA‑binding response regulator GlnL/GlnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,755,1820CG10.3% 106.6 / 5.8 39I80M (ATC→ATG) glnGtwo‑component system DNA‑binding response regulator GlnL/GlnG
Reads supporting (aligned to +/- strand):  ref base C (17/18);  new base G (2/2);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01

AGAGCTTCGACAGCGCAGACGGTGTCATGGGGCGCCTGGCACGTCAGCAACCCGACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGCTGGTCAAGCGCGCCAACCAGCACGCCCAGGAGCAGCAAGGCCTG  >  NC_002947/5755037‑5755329
                                                                                                                                                 |                                                                                                                                                   
agagCTTCGACAGCGCAGACGGTGTCATGGGGCGCCTGGCACGTCAGCAACCCGACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGtcatcatca                                                                                                                                                 <  2:1105426/150‑1 (MQ=255)
 gagCTTCGACAGCGCAGACGGTGTCATGGGGCGCCTGGCACGTCAGCAACCCGACGTGATCATTTCCGACATTCGCATGCCTGGTACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTcatcatcat                                                                                                                                                <  2:867536/150‑1 (MQ=255)
         aCAGCGCAGACGGTGTCATGGGGCGCCTGGCACGTCAGCAACCCGACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATAACCGccc                                                                                                                                        >  2:851092/1‑150 (MQ=255)
          cAGCGCAGACGGTGTCATGGGGCGCCTGGCACGTCAGCAACCCGACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCa                                                                                                                                       <  1:718758/150‑1 (MQ=255)
             cgcAGACGGTGTCATGGGGCGCCTGGCACGTCAGCAACCCGACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTc                                                                                                                                    >  1:182817/1‑150 (MQ=255)
                    ggTGTCATGGGGCGCCTGGCACGTCAGCAACCCGACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATGATGATGACCggcag                                                                                                                                       >  2:584563/1‑136 (MQ=255)
                    ggTGTCATGGGGCGCCTGGCACGTCAGCAACCCGACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATGATGATGACCggcag                                                                                                                                       <  1:584563/140‑5 (MQ=255)
                      tgtCATGGGGCGCCTGGCACGTCAGCAACCCGACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGa                                                                                                                           <  2:166469/150‑1 (MQ=255)
                             gggCGCCTGGCACGTCAGCAACCCGACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGcc                                                                                                                                         >  2:721216/1‑129 (MQ=255)
                             gggCGCCTGGCACGTCAGCAACCCGACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGcc                                                                                                                                         <  1:721216/129‑1 (MQ=255)
                               gcgcCTGGCACGTCAGCAACCCGACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGt                                                                                                                  <  2:757520/150‑1 (MQ=255)
                                 gcCTGGCACGTCAGCAACCCGACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATAACCGCCCACTCCGACCTGGACAGCGCCGTgg                                                                                                                <  1:851092/150‑1 (MQ=255)
                                                   ccGACGTGATCATTTCCGACATTCGCATTCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACAGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTg                                                                                              >  2:177798/1‑150 (MQ=255)
                                                     gACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATGATGATGACCggca                                                                                                                                        >  1:281696/1‑103 (MQ=255)
                                                     gACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATGATGATGACCggca                                                                                                                                        <  2:281696/106‑4 (MQ=255)
                                                          gATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGGGAGCAGCACCCGCGCCTGCCGGACATCATCACGACCGCACACTCCGACATGCACAGCGCCGGCGCCCCTTCCCAAGACGGAGCCTTCGa                                                                                       >  2:316577/1‑150 (MQ=255)
                                                            tCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGt                                                                                     >  2:730778/1‑150 (MQ=255)
                                                              aTTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTAc                                                                                   <  1:798476/150‑1 (MQ=255)
                                                                  ccGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTTGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGc                                                                               <  1:316577/150‑1 (MQ=255)
                                                                    gACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTg                                                                                                                             >  2:249785/1‑102 (MQ=255)
                                                                    gACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTg                                                                                                                             <  1:249785/102‑1 (MQ=255)
                                                                     aCATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCa                                                                            >  2:1146112/1‑150 (MQ=255)
                                                                       aTTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAg                                                                          <  2:117451/150‑1 (MQ=255)
                                                                            cATGCCTGGCACCAGCGGCCTCGACTTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCAtt                                                                     <  1:678063/150‑1 (MQ=255)
                                                                                   ggCACCAGCGGCCTCGACCGGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATtcgacgtc                                                              <  1:143197/150‑1 (MQ=255)
                                                                                             gCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCgg                                                    >  1:420729/1‑150 (MQ=255)
                                                                                                      tgctgGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGCTgg                                           <  2:182817/150‑1 (MQ=255)
                                                                                                          ggCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGCTGGTCaa                                       <  1:375870/150‑1 (MQ=255)
                                                                                                               aGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCAt                                                                      <  1:354185/114‑1 (MQ=255)
                                                                                                               aGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCAt                                                                      >  2:354185/1‑114 (MQ=255)
                                                                                                                    cgcgAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGCTGGTCAAGCGCGCCAAc                             >  2:193842/1‑150 (MQ=255)
                                                                                                                        agcagcACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGCTGGTCAAGCGCGCCAACCAGc                         <  2:420729/150‑1 (MQ=255)
                                                                                                                           agcaCCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGCTGGTCAAGCGCGCCAACCAGCACg                      >  2:631317/1‑150 (MQ=255)
                                                                                                                           agcaCCCGCGCCTGCCAGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGCTGGTCAAGCGCGCCAACCAGCACg                      >  2:631313/1‑150 (MQ=255)
                                                                                                                                ccGTGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGc                                              <  2:306346/121‑1 (MQ=255)
                                                                                                                                ccGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGc                                              >  1:306346/1‑121 (MQ=255)
                                                                                                                                ccGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGCTg                                            >  2:999463/1‑123 (MQ=255)
                                                                                                                                ccGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGCTg                                            <  1:999463/123‑1 (MQ=255)
                                                                                                                                       tGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGCTGGTCAAGCGCGCCAACCAGCACGCCCAGGagcagc          >  1:686192/1‑150 (MQ=255)
                                                                                                                                            gTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGt                                                                                               <  2:691884/60‑1 (MQ=255)
                                                                                                                                            gTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGt                                                                                               >  1:691884/1‑60 (MQ=255)
                                                                                                                                              catcatcatGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGCTGGTCAAGCGCGCCAACCAGCACGCCCAGGAGCAGCAAGGCCt   <  1:1161134/150‑1 (MQ=255)
                                                                                                                                               atcatcatGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATtcgac                                                                 >  2:397332/1‑87 (MQ=255)
                                                                                                                                               atcatcatGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATtcgac                                                                 <  1:397332/87‑1 (MQ=255)
                                                                                                                                               atcatcatGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGCTGGTCAAGCGCGCCAACCAGCACGCCCAGGAGCAGCAAGGCCTg  <  1:631313/150‑1 (MQ=255)
                                                                                                                                               atcatcatGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGCTGGTCAAGCGCGCCAACCAGCACGCCCAGGAGCAGCAAGGCCTg  <  1:631317/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGAGCTTCGACAGCGCAGACGGTGTCATGGGGCGCCTGGCACGTCAGCAACCCGACGTGATCATTTCCGACATTCGCATGCCTGGCACCAGCGGCCTCGACCTGCTGGCGCAGATCCGCGAGCAGCACCCGCGCCTGCCGGTCATCATCATGACCGCCCACTCCGACCTGGACAGCGCCGTGGCCTCGTACCAAGGCGGTGCCTTCGAGTACCTGCCCAAGCCATTCGACGTCGACGAAGCGGTATCGCTGGTCAAGCGCGCCAACCAGCACGCCCAGGAGCAGCAAGGCCTG  >  NC_002947/5755037‑5755329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑