Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,763,903 T→C 9.1% V408A (GTT→GCT)  PP_5057 → M23/M37 family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,763,9030TC 9.1% 117.3 / 3.0 44V408A (GTT→GCT) PP_5057M23/M37 family peptidase
Reads supporting (aligned to +/- strand):  ref base T (23/17);  new base C (2/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

GTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTT  >  NC_002947/5763756‑5764052
                                                                                                                                                   |                                                                                                                                                     
gtgtGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTg                                                                                                                                                     <  2:785281/150‑1 (MQ=255)
  gtgGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGc                                                                                                                                                   <  1:68393/150‑1 (MQ=255)
   tgGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCg                                                                                                                                                  >  2:282417/1‑150 (MQ=255)
      tATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGaaa                                                                                                                                               >  1:503106/1‑148 (MQ=255)
      tATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACa                                                                                                                                               <  1:1075535/150‑1 (MQ=255)
      tATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACa                                                                                                                                               >  2:1075535/1‑150 (MQ=255)
       aTTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAg                                                                                                                                              <  1:877946/150‑1 (MQ=255)
         tCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCg                                                                                                                                            <  2:493721/150‑1 (MQ=255)
           gcgGACTGGTTGCGCGGGGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATATCCACCGTTGGCGACAGCggt                                                                                                                                          >  2:618421/1‑150 (MQ=255)
                        gcgGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCgg                                                                                                                             >  1:35113/1‑150 (MQ=255)
                                    ttCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCtt                                                                                                                      <  1:1115889/145‑1 (MQ=255)
                                    ttCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCtt                                                                                                                      >  2:1115889/1‑145 (MQ=255)
                                                tcGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTACCACCGTTGGCGACAGCGGTGGTCAg                                                                                                                                    >  1:776886/1‑119 (MQ=255)
                                                tcGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTACCACCGTTGGCGACAGCGGTGGTCAg                                                                                                                                    <  2:776886/119‑1 (MQ=255)
                                                  gACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCcag                                                                                                   >  2:987828/1‑150 (MQ=255)
                                                   aCCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCggtgg                                                                                                                                        <  1:615798/112‑1 (MQ=255)
                                                   aCCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCggtgg                                                                                                                                        >  2:615798/1‑112 (MQ=255)
                                                      aTGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTCGCTTCCTGGTTGTGCAGGACGCGGGAGGCTTGTATTTCGCTATCCGCCAGCAgg                                                                                               >  2:31131/1‑150 (MQ=255)
                                                                      gAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGAccc                                                                               <  1:762131/150‑1 (MQ=255)
                                                                         ccTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGAAAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTc                                                                            <  2:503106/150‑1 (MQ=255)
                                                                         ccTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGCTGTCGCCAACggtggaaat                                                                                                                                    <  1:608978/94‑5 (MQ=37)
                                                                         ccTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGCTGTCGCCAACggtggaaat                                                                                                                                    >  2:608978/1‑90 (MQ=37)
                                                                            gTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCa                                                                         >  2:106592/1‑150 (MQ=255)
                                                                            gTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCa                                                                         <  1:282417/150‑1 (MQ=255)
                                                                             tACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCAGGGAGCAGGGCCGGCCTACCGACCCTTCGCAg                                                                        <  1:31131/150‑1 (MQ=255)
                                                                                  ccACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAgtggtg                                                                   >  2:990074/1‑150 (MQ=255)
                                                                                   cacaACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGCTGTCGCCAACggtgg                                                                                                                                        >  2:1190854/1‑80 (MQ=38)
                                                                                   cacaACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGCTGTCGCCAACggtgg                                                                                                                                        <  1:1190854/80‑1 (MQ=38)
                                                                                      aaCCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCgg                                                               <  2:357328/150‑1 (MQ=255)
                                                                                        ccAGAGCCTGCTCAAGAATGCCGGTGTTATCTTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCgggg                                                             <  2:917629/150‑1 (MQ=255)
                                                                                                   taaaGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGAAGACAGCCGTGGTAAGGACGCGGCAGGCTTGTATTTCGCTATACGCCAGCAGGACCGACCTACCGACCCTTCGCCGAGGTGCGGGAGCTCGTCAAAtt                                                  >  2:111536/3‑150 (MQ=255)
                                                                                                       gAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTa                                              >  2:396752/1‑150 (MQ=255)
                                                                                                       gAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTa                                              >  2:960201/1‑150 (MQ=255)
                                                                                                        aaTGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTAc                                             >  1:414991/1‑150 (MQ=255)
                                                                                                                  atatCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCt                                   >  1:756016/1‑150 (MQ=255)
                                                                                                                     tCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCCACAGCCGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAg                                >  1:811521/1‑150 (MQ=255)
                                                                                                                       gTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGtg                              >  1:204146/1‑150 (MQ=255)
                                                                                                                                 gTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGAt                    <  1:396752/150‑1 (MQ=255)
                                                                                                                                        cATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTcccc             >  2:460823/1‑150 (MQ=255)
                                                                                                                                         aTTTCCACCGTTGGCGACAGTGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCa            >  1:1139173/1‑150 (MQ=255)
                                                                                                                                         aTTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTa                                                          >  2:48001/1‑104 (MQ=255)
                                                                                                                                         aTTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTa                                                          <  1:48001/104‑1 (MQ=255)
                                                                                                                                           ttCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAgg          <  1:987828/150‑1 (MQ=255)
                                                                                                                                                 cGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAgcgc    >  1:244414/1‑150 (MQ=255)
                                                                                                                                                   ttGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCtt  <  2:713487/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTT  >  NC_002947/5763756‑5764052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑