Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,763,906 G→T 9.1% G409V (GGC→GTC)  PP_5057 → M23/M37 family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,763,9060GT 9.1% 123.1 / 3.0 45G409V (GGC→GTC) PP_5057M23/M37 family peptidase
Reads supporting (aligned to +/- strand):  ref base G (22/18);  new base T (2/2);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

GTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTA  >  NC_002947/5763758‑5764053
                                                                                                                                                    |                                                                                                                                                   
gtgGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGc                                                                                                                                                    <  1:68393/150‑1 (MQ=255)
 tgGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCg                                                                                                                                                   >  2:282417/1‑150 (MQ=255)
    tATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGaaa                                                                                                                                                >  1:503106/1‑148 (MQ=255)
    tATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACa                                                                                                                                                <  1:1075535/150‑1 (MQ=255)
    tATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACa                                                                                                                                                >  2:1075535/1‑150 (MQ=255)
     aTTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAg                                                                                                                                               <  1:877946/150‑1 (MQ=255)
       tCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCg                                                                                                                                             <  2:493721/150‑1 (MQ=255)
         gcgGACTGGTTGCGCGGGGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATATCCACCGTTGGCGACAGCggt                                                                                                                                           >  2:618421/1‑150 (MQ=255)
                      gcgGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCgg                                                                                                                              >  1:35113/1‑150 (MQ=255)
                                  ttCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCtt                                                                                                                       >  2:1115889/1‑145 (MQ=255)
                                  ttCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCtt                                                                                                                       <  1:1115889/145‑1 (MQ=255)
                                              tcGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTACCACCGTTGGCGACAGCGGTGGTCAg                                                                                                                                     <  2:776886/119‑1 (MQ=255)
                                              tcGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTACCACCGTTGGCGACAGCGGTGGTCAg                                                                                                                                     >  1:776886/1‑119 (MQ=255)
                                                gACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCcag                                                                                                    >  2:987828/1‑150 (MQ=255)
                                                 aCCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCggtgg                                                                                                                                         >  2:615798/1‑112 (MQ=255)
                                                 aCCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCggtgg                                                                                                                                         <  1:615798/112‑1 (MQ=255)
                                                    aTGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTCGCTTCCTGGTTGTGCAGGACGCGGGAGGCTTGTATTTCGCTATCCGCCAGCAgg                                                                                                >  2:31131/1‑150 (MQ=255)
                                                                    gAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGAccc                                                                                <  1:762131/150‑1 (MQ=255)
                                                                       ccTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGAAAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTc                                                                             <  2:503106/150‑1 (MQ=255)
                                                                       ccTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGCTGTCGCCAACggtggaaat                                                                                                                                     >  2:608978/1‑90 (MQ=37)
                                                                       ccTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGCTGTCGCCAACggtggaaat                                                                                                                                     <  1:608978/94‑5 (MQ=37)
                                                                          gTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCa                                                                          >  2:106592/1‑150 (MQ=255)
                                                                          gTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCa                                                                          <  1:282417/150‑1 (MQ=255)
                                                                           tACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCAGGGAGCAGGGCCGGCCTACCGACCCTTCGCAg                                                                         <  1:31131/150‑1 (MQ=255)
                                                                                ccACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAgtggtg                                                                    >  2:990074/1‑150 (MQ=255)
                                                                                 cacaACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGCTGTCGCCAACggtgg                                                                                                                                         >  2:1190854/1‑80 (MQ=38)
                                                                                 cacaACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGCTGTCGCCAACggtgg                                                                                                                                         <  1:1190854/80‑1 (MQ=38)
                                                                                    aaCCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCgg                                                                <  2:357328/150‑1 (MQ=255)
                                                                                      ccAGAGCCTGCTCAAGAATGCCGGTGTTATCTTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCgggg                                                              <  2:917629/150‑1 (MQ=255)
                                                                                                 taaaGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGAAGACAGCCGTGGTAAGGACGCGGCAGGCTTGTATTTCGCTATACGCCAGCAGGACCGACCTACCGACCCTTCGCCGAGGTGCGGGAGCTCGTCAAAtt                                                   >  2:111536/3‑150 (MQ=255)
                                                                                                     gAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTa                                               >  2:960201/1‑150 (MQ=255)
                                                                                                     gAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTa                                               >  2:396752/1‑150 (MQ=255)
                                                                                                      aaTGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTAc                                              >  1:414991/1‑150 (MQ=255)
                                                                                                                atatCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCt                                    >  1:756016/1‑150 (MQ=255)
                                                                                                                   tCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCCACAGCCGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAg                                 >  1:811521/1‑150 (MQ=255)
                                                                                                                     gTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGtg                               >  1:204146/1‑150 (MQ=255)
                                                                                                                               gTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGAt                     <  1:396752/150‑1 (MQ=255)
                                                                                                                                      cATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTcccc              >  2:460823/1‑150 (MQ=255)
                                                                                                                                       aTTTCCACCGTTGGCGACAGTGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCa             >  1:1139173/1‑150 (MQ=255)
                                                                                                                                       aTTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTa                                                           >  2:48001/1‑104 (MQ=255)
                                                                                                                                       aTTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTa                                                           <  1:48001/104‑1 (MQ=255)
                                                                                                                                         ttCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAgg           <  1:987828/150‑1 (MQ=255)
                                                                                                                                               cGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAgcgc     >  1:244414/1‑150 (MQ=255)
                                                                                                                                                 ttGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCtt   <  2:713487/150‑1 (MQ=255)
                                                                                                                                                  tGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTGCGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTa  <  2:35113/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTA  >  NC_002947/5763758‑5764053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑