Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,763,912 G→A 9.9% S411N (AGC→AAC)  PP_5057 → M23/M37 family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,763,9120GA 9.9% 104.0 / 3.7 41S411N (AGC→AAC) PP_5057M23/M37 family peptidase
Reads supporting (aligned to +/- strand):  ref base G (20/16);  new base A (2/2);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

ATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATC  >  NC_002947/5763763‑5764060
                                                                                                                                                     |                                                                                                                                                    
aTTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAg                                                                                                                                                      <  1:877946/150‑1 (MQ=255)
  tCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCg                                                                                                                                                    <  2:493721/150‑1 (MQ=255)
    gcgGACTGGTTGCGCGGGGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATATCCACCGTTGGCGACAGCggt                                                                                                                                                  >  2:618421/1‑150 (MQ=255)
                 gcgGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCgg                                                                                                                                     >  1:35113/1‑150 (MQ=255)
                             ttCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCtt                                                                                                                              >  2:1115889/1‑145 (MQ=255)
                             ttCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCtt                                                                                                                              <  1:1115889/145‑1 (MQ=255)
                                         tcGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTACCACCGTTGGCGACAGCGGTGGTCAg                                                                                                                                            >  1:776886/1‑119 (MQ=255)
                                         tcGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTACCACCGTTGGCGACAGCGGTGGTCAg                                                                                                                                            <  2:776886/119‑1 (MQ=255)
                                           gACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCcag                                                                                                           >  2:987828/1‑150 (MQ=255)
                                            aCCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCggtgg                                                                                                                                                >  2:615798/1‑112 (MQ=255)
                                            aCCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCggtgg                                                                                                                                                <  1:615798/112‑1 (MQ=255)
                                               aTGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTCGCTTCCTGGTTGTGCAGGACGCGGGAGGCTTGTATTTCGCTATCCGCCAGCAgg                                                                                                       >  2:31131/1‑150 (MQ=255)
                                                               gAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGAccc                                                                                       <  1:762131/150‑1 (MQ=255)
                                                                  ccTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGAAAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTc                                                                                    <  2:503106/150‑1 (MQ=255)
                                                                  ccTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGCTGTCGCCAACggtggaaat                                                                                                                                            <  1:608978/94‑5 (MQ=37)
                                                                  ccTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGCTGTCGCCAACggtggaaat                                                                                                                                            >  2:608978/1‑90 (MQ=37)
                                                                     gTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCa                                                                                 <  1:282417/150‑1 (MQ=255)
                                                                     gTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCa                                                                                 >  2:106592/1‑150 (MQ=255)
                                                                      tACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCAGGGAGCAGGGCCGGCCTACCGACCCTTCGCAg                                                                                <  1:31131/150‑1 (MQ=255)
                                                                           ccACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAgtggtg                                                                           >  2:990074/1‑150 (MQ=255)
                                                                            cacaACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGCTGTCGCCAACggtgg                                                                                                                                                <  1:1190854/80‑1 (MQ=38)
                                                                            cacaACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGCTGTCGCCAACggtgg                                                                                                                                                >  2:1190854/1‑80 (MQ=38)
                                                                               aaCCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCgg                                                                       <  2:357328/150‑1 (MQ=255)
                                                                                 ccAGAGCCTGCTCAAGAATGCCGGTGTTATCTTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCgggg                                                                     <  2:917629/150‑1 (MQ=255)
                                                                                            taaaGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGAAGACAGCCGTGGTAAGGACGCGGCAGGCTTGTATTTCGCTATACGCCAGCAGGACCGACCTACCGACCCTTCGCCGAGGTGCGGGAGCTCGTCAAAtt                                                          >  2:111536/3‑150 (MQ=255)
                                                                                                gAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTa                                                      >  2:396752/1‑150 (MQ=255)
                                                                                                gAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTa                                                      >  2:960201/1‑150 (MQ=255)
                                                                                                 aaTGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTAc                                                     >  1:414991/1‑150 (MQ=255)
                                                                                                           atatCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCt                                           >  1:756016/1‑150 (MQ=255)
                                                                                                              tCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCCACAGCCGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAg                                        >  1:811521/1‑150 (MQ=255)
                                                                                                                gTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGtg                                      >  1:204146/1‑150 (MQ=255)
                                                                                                                          gTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGAt                            <  1:396752/150‑1 (MQ=255)
                                                                                                                                 cATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTcccc                     >  2:460823/1‑150 (MQ=255)
                                                                                                                                  aTTTCCACCGTTGGCGACAGTGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCa                    >  1:1139173/1‑150 (MQ=255)
                                                                                                                                  aTTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTa                                                                  <  1:48001/104‑1 (MQ=255)
                                                                                                                                  aTTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTa                                                                  >  2:48001/1‑104 (MQ=255)
                                                                                                                                    ttCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAgg                  <  1:987828/150‑1 (MQ=255)
                                                                                                                                          cGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAgcgc            >  1:244414/1‑150 (MQ=255)
                                                                                                                                            ttGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCtt          <  2:713487/150‑1 (MQ=255)
                                                                                                                                             tGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTGCGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTa         <  2:35113/150‑1 (MQ=255)
                                                                                                                                                 gACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAATTTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAgg     >  2:586637/1‑150 (MQ=255)
                                                                                                                                                    aGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATc  <  1:960201/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCAGAGCCTGCTCAAGAATGCCGGTGATATCGTCAAGGCCGGTGAAGCCATTTCCACCGTTGGCGACAGCGGTGGTCAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATC  >  NC_002947/5763763‑5764060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑