Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,815,592 G→T 7.8% intergenic (+20/+71) PP_5090 → / ← PP_5091 cell division protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,815,5920GT 7.8% 162.0 / 3.1 52intergenic (+20/+71)PP_5090/PP_5091cell division protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base G (25/22);  new base T (2/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

GATGAAACCTGGTACCGCGTGCTGGTTGGGCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACAGCACC  >  NC_002947/5815444‑5815726
                                                                                                                                                    |                                                                                                                                      
gATGAAACCTGGTACCGCGTGCTGGTTGGGCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGc                                                                                                                                       >  1:1082973/1‑150 (MQ=255)
                      tGGTTGGGCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTtat                                                                                                                 >  1:1131979/1‑150 (MQ=255)
                        gTTGGGCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTatat                                                                                                               >  2:641117/1‑150 (MQ=255)
                        gTTGGGCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTatat                                                                                                               >  1:66375/1‑150 (MQ=255)
                            ggCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATg                                                                                                                                   <  2:292677/126‑1 (MQ=255)
                            ggCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATg                                                                                                                                   >  1:292677/1‑126 (MQ=255)
                            ggCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGAc                                                                                                           <  2:66375/150‑1 (MQ=255)
                                  tCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCAtt                                                                                                     <  1:343487/150‑1 (MQ=255)
                                    aGCAACCGCGAGCAGTTGACAGGGACACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTTGATTGGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGc                                                                                                   <  1:625216/150‑1 (MQ=255)
                                     gCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGAt                                                                                                                                    <  1:1101643/116‑1 (MQ=255)
                                     gCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGAt                                                                                                                                    >  2:1101643/1‑116 (MQ=255)
                                                 aGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGa                                                                                                                                     <  1:794309/103‑1 (MQ=255)
                                                 aGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGa                                                                                                                                     >  2:794309/1‑103 (MQ=255)
                                                  gTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGATCGGCAACCTGTTGCTGCAACAGCGGCAGAACCGTCAGCAACGAAGAAGACCTTGCCGCAACGATGGGACAAGTCGTTCTTTATATGGTCAGCATTACCCGTAAAGGGGCTg                                                                                     >  2:445239/1‑150 (MQ=255)
                                                   ttGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGcc                                                                                                                         >  2:829933/1‑113 (MQ=255)
                                                   ttGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGcc                                                                                                                         <  1:829933/113‑1 (MQ=255)
                                                         ggggCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAagca                                                                              <  2:381833/150‑1 (MQ=255)
                                                         ggggCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAagca                                                                              <  2:123231/150‑1 (MQ=255)
                                                              acaGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGTCGCATCGCTGCGGCAAg                                                                                                                            <  1:14240/99‑1 (MQ=38)
                                                              acaGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGTCGCATCGCTGCGGCAAg                                                                                                                            >  2:14240/1‑99 (MQ=38)
                                                                  aaGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCa                                                                     >  1:475604/1‑150 (MQ=255)
                                                                     cagcTGGCTGGGGCCGGGTTCAGCAACCTGTTTCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCg                                                                  >  1:192990/1‑150 (MQ=255)
                                                                      agcTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGt                                                                 >  1:482389/1‑150 (MQ=255)
                                                                               gggCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAgccccgc                                                        <  1:600646/150‑1 (MQ=255)
                                                                                 gCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGc                                                                                                                                  >  2:1126890/1‑74 (MQ=255)
                                                                                 gCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGc                                                                                                                                  <  1:1126890/74‑1 (MQ=255)
                                                                                       ttCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGTCGCATCGCTGCGGCAAg                                                                                                                            >  1:1148495/1‑74 (MQ=25)
                                                                                       ttCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGTCGCATCGCTGCGGCAAg                                                                                                                            <  2:1148495/74‑1 (MQ=25)
                                                                                            cAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACTAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCttt                                           <  2:1131979/150‑1 (MQ=255)
                                                                                             aaCCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTc                                          >  2:775011/1‑150 (MQ=255)
                                                                                              aCCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGCAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCg                                         <  1:347096/150‑1 (MQ=255)
                                                                                                     gctgcAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACATCATTGc                                                                                                   >  1:750162/1‑85 (MQ=255)
                                                                                                     gctgcAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGc                                                                                                   <  2:750162/85‑1 (MQ=255)
                                                                                                     gctgcAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCt                                  >  2:417075/1‑150 (MQ=255)
                                                                                                         cAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCt                              >  1:161067/1‑150 (MQ=255)
                                                                                                          aaCAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTg                             >  2:202584/1‑150 (MQ=255)
                                                                                                           aCAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGt                            <  2:1082973/150‑1 (MQ=255)
                                                                                                                          tCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTc                                               <  1:562095/116‑1 (MQ=255)
                                                                                                                          tCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTc                                               >  2:562095/1‑116 (MQ=255)
                                                                                                                            aGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCa           <  1:202584/150‑1 (MQ=255)
                                                                                                                            aGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCa           <  1:641117/150‑1 (MQ=255)
                                                                                                                            aGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGATGTTCACCGTCAAgggctga           >  2:558498/1‑144 (MQ=255)
                                                                                                                              tAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCt                                             <  2:230163/114‑1 (MQ=255)
                                                                                                                              tAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCt                                             >  1:230163/1‑114 (MQ=255)
                                                                                                                                  gaagaaGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACAGc     >  1:567450/1‑150 (MQ=255)
                                                                                                                                  gaagaaGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACAGc     >  2:1061244/1‑150 (MQ=255)
                                                                                                                                    agaagGCCTTGCCGCAGCGTTGCGACAAGGCCTTCTTTATATGGAACGCCCAGCCGCTGCATGGGCTGGGATTCAGCCCCGCCCCCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACAGCAc   <  1:445239/150‑1 (MQ=255)
                                                                                                                                    agaagGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACa       >  1:1102358/1‑146 (MQ=255)
                                                                                                                                    agaagGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACa       <  2:1102358/146‑1 (MQ=255)
                                                                                                                                     gaagGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACAGCAcc  >  1:386631/1‑150 (MQ=255)
                                                                                                                                           cTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGaa               >  2:707669/1‑131 (MQ=255)
                                                                                                                                           cTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGaa               <  1:707669/131‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                      
GATGAAACCTGGTACCGCGTGCTGGTTGGGCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACAGCACC  >  NC_002947/5815444‑5815726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑