| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,879,191 | T→C | 6.9% | intergenic (‑139/+44) | PP_5152 ← / ← ycgM | hypothetical protein/isomerase/hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,879,191 | 0 | T | C | 6.9% | 168.5 / 2.7 | 58 | intergenic (‑139/+44) | PP_5152/ycgM | hypothetical protein/isomerase/hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (22/31); new base C (2/2); total (24/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
GAGGATGGCATGCGGTGATCCTGGGAAAATGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCTCGTCACCGACATTGAACGGCCCGACGCCGGCCGGGGTACCGGTGAGGA > NC_002947/5879042‑5879335 | gaggaTGGCATGCGGTGATCCTGGGAAAATGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCt < 2:994355/150‑1 (MQ=255)gaggaTGGCATGCGGTGATCCTGGGAAAATGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCt > 1:295471/1‑150 (MQ=255) ggCATGCGGTGATCCTGGGAAAATGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGa < 2:267219/150‑1 (MQ=255) gCATGCGGTGATCCTGGGAAAATGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAg > 2:1069698/1‑150 (MQ=255) cATGCGGTGATCCTGGGAAAATGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATACAAGGCCCTGTGCAACCCCTGTAGGAGc < 1:284208/150‑1 (MQ=255) tGCGGTGATCCTGGGAAAATGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCgg < 1:881889/150‑1 (MQ=255) cGGTGATCCTGGGAAAATGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTCAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTAGTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGt < 1:699249/150‑1 (MQ=255) cGGTGATCCTGGGAAAATGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGt < 1:228769/150‑1 (MQ=255) cGGTGATCCTGGGAAAATGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGt < 1:288114/150‑1 (MQ=255) gTGATCCTGGGAAAATGTGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCg < 1:147017/145‑1 (MQ=255) gTGATCCTGGGAAAATGTGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCg > 2:147017/1‑145 (MQ=255) gTGATCCTGGGAAAATGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGttt < 2:1183884/150‑1 (MQ=255) ggAAAATGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCgaa < 2:295471/150‑1 (MQ=255) aaTGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGaaa > 1:637241/1‑150 (MQ=255) aTGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGCGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAg < 2:117016/150‑1 (MQ=255) gTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGAcgc < 1:956108/150‑1 (MQ=255) gTAGGTGCGCGCATCAGCCGCATTCAGGGCGAAAAAGTCATAAATGGGGCCGACCCTTTCGCCGGTAAGCCCCAGCTCAACGTACTGCACAATATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTGCCCCCGAAGAAAGCGAcac < 2:368466/150‑3 (MQ=255) gcgcATTATCCGGATTGGGTGGGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGTACAGTATTCAAGGCCCTGTGCAACGCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGa < 1:161491/150‑1 (MQ=255) cgcATTATCCGGATTGGGTGTGAAAGATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTACACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAg > 1:1064085/1‑150 (MQ=255) tATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGAcgc < 1:558123/136‑1 (MQ=255) tATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGAcgc > 2:558123/1‑136 (MQ=255) tATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCaaa < 1:763454/150‑1 (MQ=255) ttCCGGTTTGGGTGTGCAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAAGCCCTGTGCAACCCCTGTAGGAGCGGGTTTGCCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAg < 2:157067/149‑1 (MQ=255) ggATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGAcgc > 1:58630/1‑131 (MQ=255) ggATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGAcgc < 2:58630/131‑1 (MQ=255) ttGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCgg < 1:1069698/150‑1 (MQ=255) gggTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCgaaga < 1:258747/117‑1 (MQ=255) gggTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCgaaga > 2:258747/1‑117 (MQ=255) aaaaaTCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAg > 2:925991/1‑150 (MQ=255) aaaaaTCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAg > 2:925925/1‑150 (MQ=255) aaaaaTCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAg > 2:904648/1‑150 (MQ=255) cGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTc < 1:925925/150‑1 (MQ=255) cGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTc < 1:925991/150‑1 (MQ=255) cAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCAAAGGTACGGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCg > 1:834549/1‑150 (MQ=255) aGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCgg < 2:59252/150‑1 (MQ=255) gggCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGc > 2:1192565/1‑150 (MQ=255) ggccggccTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCa > 2:649486/1‑150 (MQ=255) gCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAg < 2:283539/150‑1 (MQ=255) ccGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGc < 1:944523/150‑1 (MQ=255) aCCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCTCGTCAc > 1:1190705/1‑150 (MQ=255) cccGTGCGCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGTGTCAAAGCACCCGGCGTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCTCGtgccc > 2:553303/1‑146 (MQ=255) cTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAg > 2:707184/1‑114 (MQ=255) cTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAg < 1:707184/114‑1 (MQ=255) tATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCTCGTCACCGACATTGAACGGCCCGACgccgg > 2:978904/1‑150 (MQ=255) cAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAg > 2:912477/1‑69 (MQ=255) cAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAg < 1:912477/69‑1 (MQ=255) ccTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCTCGTCACCGACATTGAACGGCCCGACGCCGGCCGGGGTAcc > 2:1166478/1‑150 (MQ=255) tgtgCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCTCGTCACCGACATTGAACGGc > 1:40426/1‑128 (MQ=255) tgtgCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCTCGTCACCGACATTGAACGGc < 2:40426/128‑1 (MQ=255) gtgaacccCCGTAGGAGCGGGTTTACCCGCGAAGAggcccgtcaggcaaaccaaaaaaagccccgccagtgcaaactggcggggc > 2:460161‑M2/4‑35 (MQ=255) gtgaacccCCGTAGGAGCGGGTTTACCCGCGAAGAggcccgtcaggcaaaccaaaaaaagccccgccagtgcaaactggcggggc < 1:460161‑M2/82‑51 (MQ=255) gCAACCGCGGTCGGCGCGAGTTTACCCGCGAAGAAAGCGACTCCGCTTCGAGACAAAGCCCCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCTCGTCGCCGACATTGAACGGCCCGACGCCGGCCGGGGTACCGGTGa < 2:1049042/150‑1 (MQ=255) gCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCTCGTCACCGACATTGAACGGCCCGACGCCGGCCGGGGTACCGGTGa < 2:637241/150‑1 (MQ=255) cAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCTCGTCACCGACATTGAACGGCCCGACGCCGGCCGGGGTACCg > 1:1106473/1‑145 (MQ=255) cAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCTCGTCACCGACATTGAACGGCCCGACGCCGGCCGGGGTACCg < 2:1106473/145‑1 (MQ=255) cAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCTCGTCACCGACATTGAACGGCCCGACGCCGGCCGGGGTACCGGTGAg < 1:649486/150‑1 (MQ=255) aaCCCCTGTAGGAGCGAGCTTACCCGCGAAGAAAGCGACCCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCCCCTCACCGACATTAAACGGCCCGACTCCGGCCGGCGTCCCGGTGAgg > 2:601386/1‑150 (MQ=255) aCCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCTCGTCACCGACATTGAACGGCCCGACGCCGGCCGGGGTACCGGTGAGGa < 1:1166478/150‑1 (MQ=255) ccccCGTAGGAGCGGGTTTACCCGCGAAGAggcccgtcaggcaaaccaaaaaaagccccgccagtgcaaactggcgggg > 1:164650‑M2/1‑30 (MQ=255) ccccCGTAGGAGCGGGTTTACCCGCGAAGAggcccgtcaggcaaaccaaaaaaagccccgccagtgcaaactggcgggg < 2:164650‑M2/79‑50 (MQ=255) | GAGGATGGCATGCGGTGATCCTGGGAAAATGGGCGTAGGTGCGCGCATTATCCGGATTGGGTGTGAAAAATCGGTAAACAGGGCCGGCCTCTTCGCCGGTAAACCCGTGCCCAAGGTACTGCACAGTATTCAAGGCCCTGTGCAACCCCTGTAGGAGCGGGTTTACCCGCGAAGAAAGCGACGCGGTTTCGAGTCAAAGCACCCGGCTTTCGAAGCGGCTCACACCCGGCAGCTCCAGCACCAGCTCGTCACCGACATTGAACGGCCCGACGCCGGCCGGGGTACCGGTGAGGA > NC_002947/5879042‑5879335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |