Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,897,998 G→C 8.6% A982A (GCC→GCG)  PP_5173 ← RND family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,897,9980GC 8.6% 165.7 / 5.7 55A982A (GCC→GCG) PP_5173RND family transporter
Reads supporting (aligned to +/- strand):  ref base G (28/22);  new base C (3/2);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

TTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCT  >  NC_002947/5897849‑5898133
                                                                                                                                                     |                                                                                                                                       
ttCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAg                                                                                                                                         <  1:1067233/150‑1 (MQ=255)
 tCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAgg                                                                                                                                        <  1:199099/150‑1 (MQ=255)
     gggTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCAt                                                                                                                                    >  2:640602/1‑150 (MQ=255)
      ggTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATg                                                                                                                                   <  2:1144357/150‑1 (MQ=255)
           aCCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTTGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCTACGATGATCCCGCCAATCATGGCGTAGGCGATGGGGcc                                                                                                                              >  1:182348/1‑150 (MQ=255)
             ccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATgattggcg                                                                                                                            <  1:640602/150‑8 (MQ=255)
             ccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATga                                                                                                                                  >  2:841976/1‑143 (MQ=255)
             ccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATga                                                                                                                                  <  1:841976/144‑2 (MQ=255)
                 tCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGaa                                                                                                                        <  2:441889/150‑1 (MQ=255)
                      caaGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCt                                                                                                                   >  1:593707/3‑150 (MQ=255)
                       cAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGAGTAGGCCATGGGGCCCCAGAACACCTc                                                                                                                  >  2:29628/1‑150 (MQ=255)
                          gggCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCAt                                                                                                                                    >  2:592720/1‑129 (MQ=255)
                          gggCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCAt                                                                                                                                    <  1:592720/129‑1 (MQ=255)
                                tatGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAAt                                                                                                         >  2:478399/1‑150 (MQ=255)
                                       cTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGAGTACACAAGTGTGGGCCAGAACACCTCGCGTGCAATCGGGATc                                                                                                  >  2:286214/1‑150 (MQ=255)
                                        ttCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACATACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCa                                                                                                 >  2:874002/1‑150 (MQ=255)
                                             gCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCGCAGAACACCTCGCGTGCAATCGGGATCATGccc                                                                                            >  1:593953/1‑150 (MQ=255)
                                             gCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGccc                                                                                            >  1:726734/1‑150 (MQ=255)
                                                 gtTTCGGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCTACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGc                                                                                        <  2:182348/150‑1 (MQ=255)
                                                  tttCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATg                                                                                               >  2:609416/1‑142 (MQ=255)
                                                  tttCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATg                                                                                               <  1:609416/142‑1 (MQ=255)
                                                      tGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCt                                                                                                                   >  1:578301/1‑118 (MQ=255)
                                                      tGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCt                                                                                                                   <  2:578301/118‑1 (MQ=255)
                                                        cGGCTCGCGGATCTTGTACCGGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCATAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgccgc                                                                                 <  1:539344/150‑1 (MQ=255)
                                                        cGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgccgc                                                                                 >  1:435085/1‑150 (MQ=255)
                                                           cTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGccc                                                                                                                             >  1:860650/1‑103 (MQ=255)
                                                           cTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGccc                                                                                                                             <  2:860650/103‑1 (MQ=255)
                                                            tCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGaa                                                                                                                        <  1:249317/107‑1 (MQ=255)
                                                            tCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGaa                                                                                                                        >  2:249317/1‑107 (MQ=255)
                                                              gcgGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgccgccgccgc                                                                           <  1:259060/150‑1 (MQ=255)
                                                                           cAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATc                                                              >  1:474982/1‑150 (MQ=255)
                                                                            aGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGc                                                                                        >  1:696024/1‑123 (MQ=255)
                                                                            aGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGc                                                                                        <  2:696024/123‑1 (MQ=255)
                                                                               ccACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATcggcc                                                          >  1:839350/1‑150 (MQ=255)
                                                                                cACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATcggccg                                                         >  2:714255/1‑150 (MQ=255)
                                                                                 aCGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATcggccgg                                                        >  1:341391/1‑150 (MQ=255)
                                                                                                  gAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGc                                                                                        <  2:479347/101‑1 (MQ=255)
                                                                                                  gAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGc                                                                                        >  1:479347/1‑101 (MQ=255)
                                                                                                  gAACAGCAGCGTCAGCAAGGTCGCCACGATGATCACGCCAATCATGGCGTAGGCCCTGGGGCCGCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCTGCCGGCTGCGGTGGTTGGTGGc                                       >  1:184643/1‑150 (MQ=255)
                                                                                                        cagcGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTccac                                 <  1:874002/150‑1 (MQ=255)
                                                                                                               aGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCCCGCCGCCGCCGCGGTCAGCAGGAACGGCCGGCGTCGGTGGTTGGTGGCCTCCACCAACGcc                          >  2:66336/1‑149 (MQ=255)
                                                                                                                       cGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgccgccgccg                                                                            <  1:154163/92‑1 (MQ=255)
                                                                                                                       cGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgccgccgccg                                                                            >  2:154163/1‑92 (MQ=255)
                                                                                                                       cGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACgg                  <  1:207230/150‑1 (MQ=255)
                                                                                                                          cACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGa               <  2:474982/150‑1 (MQ=255)
                                                                                                                          cACGATGATCCCGCCAATCATGGAGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGa               <  1:29628/150‑1 (MQ=255)
                                                                                                                             gatgatCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTcca                                  <  1:342740/128‑1 (MQ=255)
                                                                                                                             gatgatCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTcca                                  >  2:342740/1‑128 (MQ=255)
                                                                                                                             gatgatCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACaa            <  2:102502/150‑1 (MQ=255)
                                                                                                                                  tCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCt       >  2:1177698/1‑150 (MQ=255)
                                                                                                                                  tCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCt       >  2:312268/1‑150 (MQ=255)
                                                                                                                                   cccGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTg      >  1:513634/1‑150 (MQ=255)
                                                                                                                                     cGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTggg    <  2:726734/150‑1 (MQ=255)
                                                                                                                                     cGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGGCGTGGCCACCACCGCATCCCACGGCGACAAGCCCTggg    >  2:1188909/1‑150 (MQ=255)
                                                                                                                                       ccAATCATGGCGTAGGCCATGGGGCCCCAGAACCCCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCt  <  2:472085/150‑1 (MQ=255)
                                                                                                                                              tGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgcc                                                                                   >  1:570982/1‑62 (MQ=255)
                                                                                                                                              tGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgcc                                                                                   <  2:570982/62‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                       
TTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCT  >  NC_002947/5897849‑5898133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑