| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 417,378 | A→G | 7.0% | Q362R (CAG→CGG) | waaG → | lipopolysaccharide glucosyltransferase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 417,378 | 0 | A | G | 7.0% | 170.2 / 2.5 | 57 | Q362R (CAG→CGG) | waaG | lipopolysaccharide glucosyltransferase I |
| Reads supporting (aligned to +/- strand): ref base A (26/27); new base G (2/2); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
CGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGGCG > NC_002947/417229‑417521 | cGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCgca > 1:693200/1‑150 (MQ=255) gCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTgcggccgtctg < 2:599961/150‑7 (MQ=255) gCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCAtgctg < 1:758588/150‑1 (MQ=255) ggTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGa < 2:875616/150‑1 (MQ=255) gCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGt > 1:395393/1‑150 (MQ=255) tGGAAGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGGGCAGGCGCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCGTTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGa > 2:676526/1‑150 (MQ=255) gACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATc > 2:1140264/1‑150 (MQ=255) gACGAGCAGTCAGAACAAGCGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTGACACAATGCCCTGGCATTTGCTGACAGCGCCGATCTGTACAGCATCCCGCACCATGCGGCCGACGTGATc < 2:513073/150‑1 (MQ=255) gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctgtacagat > 2:525825/1‑134 (MQ=255) gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctgtacagat < 1:525825/141‑8 (MQ=255) gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTggg < 1:715569/150‑1 (MQ=255) gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTggg > 1:288434/1‑150 (MQ=255) cAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGa < 2:498193/128‑1 (MQ=255) cAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGa > 1:498193/1‑128 (MQ=255) agagCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGc < 1:1011403/150‑1 (MQ=255) tCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCt < 1:956072/150‑1 (MQ=255) aaCGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGGTCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGa < 1:553832/150‑1 (MQ=255) ggCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCa‑aaaaa > 2:912861/1‑133 (MQ=255) ggCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCa‑aaaaa < 1:912861/133‑1 (MQ=255) cTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTg < 2:774148/150‑1 (MQ=255) aTCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGc < 1:1140264/150‑1 (MQ=255) aTCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTAAAAAAAAAAAA‑AAAAACCGCATGAAGCTGATATTGGc < 2:1027282/150‑1 (MQ=255) tGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCa < 2:776165/121‑1 (MQ=255) tGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCa > 1:776165/1‑121 (MQ=255) cAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAAc < 1:352750/150‑1 (MQ=255) ctgCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTggg > 2:1141966/3‑150 (MQ=255) ccGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTggg > 2:907642/1‑150 (MQ=255) gcgTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGa < 2:490933/83‑1 (MQ=255) gcgTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGa > 1:490933/1‑83 (MQ=255) gcgTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgg > 1:398621/1‑89 (MQ=255) gcgTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgg < 2:398621/89‑1 (MQ=255) cgTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTg < 2:395393/150‑1 (MQ=255) gCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATg > 2:290969/1‑150 (MQ=255) cAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGcc > 1:579279/1‑138 (MQ=255) cAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGcc < 2:579279/138‑1 (MQ=255) gcgcAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGc < 2:965992/104‑1 (MQ=255) gcgcAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGc > 1:965992/1‑104 (MQ=255) gcgcAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAAcc > 2:904595/1‑110 (MQ=255) gcgcAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAAcc < 1:904595/110‑1 (MQ=255) gcgcAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGcc > 2:426074/1‑150 (MQ=255) gCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCACAAAGACTGC‑GCAAGCTTATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGc > 2:347210/1‑150 (MQ=255) gCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGcc < 2:471363/92‑1 (MQ=255) gCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGcc > 1:471363/1‑92 (MQ=255) gCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACTCCGTGGAGGCGCTGc > 1:467985/1‑150 (MQ=255) aTTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCtt > 1:1007981/1‑98 (MQ=255) aTTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCtt < 2:1007981/98‑1 (MQ=255) cTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGa < 1:536309/150‑1 (MQ=255) cTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGa < 2:693200/150‑1 (MQ=255) tGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAg < 1:1141966/150‑1 (MQ=255) aTAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGt < 1:426074/150‑1 (MQ=255) gcgcCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTa > 1:1145203/1‑150 (MQ=255) gcCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATc > 1:506438/1‑150 (MQ=255) cGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATcgc > 2:323993/1‑150 (MQ=255) cgtcggcAGCATGCTGCGGCATGCTGTCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAg < 2:1014336/132‑1 (MQ=255) cgtcggcAGCATGCTGCGGCATGCTGTCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAg > 1:1014336/7‑138 (MQ=255) tACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGcc < 2:1193616/104‑1 (MQ=255) tACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGcc > 1:1193616/1‑104 (MQ=255) aTGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGgc > 2:1086540/1‑150 (MQ=255) tGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGgcg > 1:946066/1‑150 (MQ=255) | CGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCA‑AAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGGCG > NC_002947/417229‑417521 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |