Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,923,762 A→C 6.2% L242R (CTG→CGG)  gcvP‑II ← glycine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,923,7620AC 6.2% 167.5 / 2.0 58L242R (CTG→CGG) gcvP‑IIglycine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (23/31);  new base C (2/2);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGACAGGCGAAATAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATCAGCTCAAAGCCGAAGCCTT  >  NC_002947/5923616‑5923909
                                                                                                                                                  |                                                                                                                                                   
cgcgACAGGCGAAATAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAggg                                                                                                                                                  <  1:314356/150‑1 (MQ=255)
       ggCGAAATAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCctgctg                                                                                                                                           >  1:1112228/1‑150 (MQ=255)
        gCGAAATAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCATGGTctgcttg                                                                                                                                          <  2:952215/150‑3 (MQ=255)
        gCGAAATAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTgctgg                                                                                                                                          >  2:1075898/1‑150 (MQ=255)
               aGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTAAGCGTCTACGCAGTCCAGGGCCTGCTGGCTGTGCa                                                                                                                                   >  2:615235/1‑150 (MQ=255)
                ggCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTTGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCaa                                                                                                                                  <  2:154654/150‑1 (MQ=255)
                   cgcgTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTg                                                                                                                               <  1:1181479/150‑1 (MQ=255)
                     cgTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGt                                                                                                                             <  2:949199/150‑1 (MQ=255)
                      gTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTc                                                                                                                            <  2:157880/150‑1 (MQ=255)
                             ccgccgTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGc                                                                                                                     >  1:569461/1‑150 (MQ=255)
                                       ccATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAgg                                                                                                                                                   <  1:385002/110‑1 (MQ=255)
                                       ccATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAgg                                                                                                                                                   >  2:385002/1‑110 (MQ=255)
                                          tCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTgctggct                                                                                                                                        >  1:1129064/1‑118 (MQ=255)
                                          tCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTgctggct                                                                                                                                        <  2:1129064/118‑1 (MQ=255)
                                             gCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCa                                                                                                     >  2:33586/1‑150 (MQ=255)
                                               acacCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCCTGGCctgcgtag                                                                                                                                         <  1:1118357/112‑5 (MQ=255)
                                               acacCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCCTGGCctgcgtag                                                                                                                                         >  2:1118357/1‑108 (MQ=255)
                                                 acCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTc                                                                                                 <  1:1075898/150‑1 (MQ=255)
                                                 acCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTc                                                                                                 >  1:1099345/1‑150 (MQ=255)
                                                                 gAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCggg                                                                                 <  1:738311/150‑1 (MQ=255)
                                                                   gCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTa                                                                               <  1:637118/150‑1 (MQ=255)
                                                                    cccAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTAc                                                                              <  1:615235/150‑1 (MQ=255)
                                                                          accacATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACtcatga                                                                        <  2:569461/150‑6 (MQ=255)
                                                                          accacATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTgcagc                                                                        <  2:419553/150‑1 (MQ=255)
                                                                                  ggCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGcc                                                                <  1:33586/150‑1 (MQ=255)
                                                                                       cGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTGCTGCAGCAAGGCGCCCAaca                                                           >  2:870737/1‑150 (MQ=255)
                                                                                           ctctCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCaaa                                                                                                            >  1:1078909/1‑97 (MQ=255)
                                                                                           ctctCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCaaa                                                                                                            <  2:1078909/97‑1 (MQ=255)
                                                                                           ctctCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGa                                                       <  2:1099345/150‑1 (MQ=255)
                                                                                                cGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCt                                                  >  1:969695/1‑150 (MQ=255)
                                                                                                 gggCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTg                                                                                           >  2:475321/1‑108 (MQ=255)
                                                                                                 gggCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTg                                                                                           <  1:475321/108‑1 (MQ=255)
                                                                                                 gggCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCtt                                                 >  2:941966/1‑150 (MQ=255)
                                                                                                  ggCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTg                                                <  2:236723/150‑1 (MQ=255)
                                                                                                  ggCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCCTg                                                <  1:1206919/150‑1 (MQ=255)
                                                                                                  ggCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTAAGCAGGCCAGGGCATGCTGGCTGTGCAACTTGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTg                                                <  2:905120/150‑1 (MQ=255)
                                                                                                      gTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCaa                                            <  1:941966/150‑1 (MQ=255)
                                                                                                           aGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCCCCTCTCCCACTGGAGCGGGGGTGTCCGGGGACTGCAGCCAGGCGCCGAACACCGAGCACTTGGGAAGGTTa                                       >  1:274580/1‑150 (MQ=255)
                                                                                                               ccaccaGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAACCCGCCGAACGCGTAGTGCTTGGCAAGGTTACCca                                   <  2:352390/150‑1 (MQ=255)
                                                                                                                 accaGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCgg                                                                                  <  1:484837/101‑1 (MQ=255)
                                                                                                                 accaGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCgg                                                                                  >  2:484837/1‑101 (MQ=255)
                                                                                                                  ccaGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAg                                >  1:744090/1‑150 (MQ=255)
                                                                                                                     ggCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAAt                             <  1:111407/150‑1 (MQ=255)
                                                                                                                        tcggcagGTCAGCGGCTCCGCAGGCCCCGGCCTGCTGGTGGTGCATCTTGTCGATCAGCGTGGGCAAATCGCGCACTTCGTCGTGGGTTTCCGGGTACTGCAGCAAGGCTCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATcga                          <  2:563340/147‑1 (MQ=255)
                                                                                                                        tCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGcc                                                                <  1:773113/112‑1 (MQ=255)
                                                                                                                        tCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGcc                                                                >  2:773113/1‑112 (MQ=255)
                                                                                                                        tCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATcga                          <  2:969695/150‑1 (MQ=255)
                                                                                                                          agcagGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCcac                                  <  2:981453/140‑1 (MQ=255)
                                                                                                                          agcagGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCcac                                  >  1:981453/1‑140 (MQ=255)
                                                                                                                           gcagGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAaca                                                           >  2:531472/1‑114 (MQ=255)
                                                                                                                           gcagGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAaca                                                           <  1:531472/114‑1 (MQ=255)
                                                                                                                               gTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGgc                                                                   >  2:107075/1‑102 (MQ=255)
                                                                                                                               gTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGgc                                                                   <  1:107075/102‑1 (MQ=255)
                                                                                                                                      gCTACGCAGGCCAGTGCCTGCTGGCTGTGCAACTGGTCCATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCCAGGTTATCCACACAATCCCCGATCAGCTCACAg            >  2:55729/1‑150 (MQ=255)
                                                                                                                                       gccagCAGGCCCTGGCCTGCTGGCTGTGCAACTGGTCGATCAGCAGGCATAGATTGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAAAACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATCAGCTCAAAGc           >  1:634173/5‑150 (MQ=255)
                                                                                                                                       cTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATCAGCTCAAAGc           >  2:713779/1‑150 (MQ=255)
                                                                                                                                               gCCAGGGCCTGATGGATGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATCAGCTCAAAGCCGAAGCCt   >  2:956142/1‑150 (MQ=255)
                                                                                                                                                ccAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATCAGCTCAAAGCCGAAGCCtt  <  1:861227/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGCGACAGGCGAAATAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATCAGCTCAAAGCCGAAGCCTT  >  NC_002947/5923616‑5923909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑