Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,966,388 A→T 10.4% intergenic (‑401/+29) PP_5232 ← / ← amtB hypothetical protein/ammonium transporter AmtB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,966,3880AT10.4% 140.5 / 7.8 48intergenic (‑401/+29)PP_5232/amtBhypothetical protein/ammonium transporter AmtB
Reads supporting (aligned to +/- strand):  ref base A (19/24);  new base T (2/3);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

TTCGGCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGTAGGTGAC  >  NC_002947/5966240‑5966533
                                                                                                                                                    |                                                                                                                                                 
ttCGGCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCaa                                                                                                                                                  <  2:797691/150‑1 (MQ=255)
ttCGGCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGAAAGTTTTTTACCTACGTCACGAACGTGTAGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCaa                                                                                                                                                  >  1:617503/1‑150 (MQ=255)
         cATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCaa                                                                                                                                         <  1:377787/150‑1 (MQ=255)
          aTTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCTTGCCGGGCatt                                                                                                                                        <  1:556970/150‑3 (MQ=255)
              tAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGtttt                                                                                                                                    >  1:473414/1‑150 (MQ=255)
               aGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAACTTGCCCGGCTTGCCGGGCattttttt                                                                                                                                   <  1:602291/150‑8 (MQ=255)
                gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTa                                                                                                                                  <  1:838812/150‑1 (MQ=255)
                          ggAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCa                                                                                                                        <  2:1159357/150‑1 (MQ=255)
                                    aagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGtt                                                                                                              >  2:642181/1‑150 (MQ=255)
                                     agcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCTTGCCGGGCattt                                                                                                                                       >  2:524469/1‑121 (MQ=255)
                                     agcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCTTGCCGGGCattt                                                                                                                                       <  1:524469/124‑4 (MQ=255)
                                        aCCATAAACGCCCTGCGGCTTGAAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGGTTTTACCTCGTCGCAGTTACAGGTTGTAg                                                                                                          <  2:617503/150‑1 (MQ=255)
                                         ccATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGc                                                                                                         <  1:1138920/150‑1 (MQ=255)
                                          cATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACcgc                                                                                                                              <  1:1091215/128‑1 (MQ=255)
                                          cATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACcgc                                                                                                                              >  2:1091215/1‑128 (MQ=255)
                                          cATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGCGTCGAATAAACCGTTTGCCGTGCGGGCACTCCTGCAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTTGCAGTTACAGGTTGTAGcc                                                                                                        >  1:447774/1‑150 (MQ=255)
                                           aTAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCcg                                                                                                       >  1:1043642/1‑150 (MQ=255)
                                             aaaCGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGtt                                                                                                                     >  1:255816/1‑134 (MQ=255)
                                             aaaCGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGtt                                                                                                                     <  2:255816/134‑1 (MQ=255)
                                                     tGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTtgt                                                                                             >  1:496289/1‑150 (MQ=255)
                                                     tGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTtgt                                                                                             >  2:1101434/1‑150 (MQ=255)
                                                         gCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAGAAACTGGCAAAAACTTGCCCGGCTTGCCGGGCATtttttt                                                                                                                                   >  2:1144227/1‑108 (MQ=37)
                                                               atgTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAg                                                                                   <  1:806527/148‑1 (MQ=255)
                                                                     tttACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAg                                                                             <  1:572478/150‑1 (MQ=255)
                                                                      ttACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTCTTTATCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAgg                                                                            <  2:1012082/150‑1 (MQ=255)
                                                                           tACGTCACGAACGTGTCGAATATACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACGGCGTCGCCGTTACCGGTTGTAGCCGCGCTCGTTGTGATGAGCCAGGTCGGCGccgac                                                                       <  2:447774/150‑1 (MQ=255)
                                                                           tACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGccgac                                                                       <  2:473414/150‑1 (MQ=255)
                                                                            aCGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGccgacc                                                                      <  2:1143288/150‑1 (MQ=255)
                                                                            aCGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGccgacc                                                                      >  1:679698/1‑150 (MQ=255)
                                                                              gTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGccgaccga                                                                    <  1:642181/150‑1 (MQ=255)
                                                                               tCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCcgaccgac                                                                   <  2:1043642/150‑1 (MQ=255)
                                                                                 aCGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGActc                                                                 <  2:496289/150‑1 (MQ=255)
                                                                                   gAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtctt                                                               >  1:525901/1‑150 (MQ=255)
                                                                                       gtgtCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttctt                                                            <  1:223014/149‑1 (MQ=255)
                                                                                       gtgtCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttctt                                                            >  2:223014/1‑149 (MQ=255)
                                                                                          tCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCttcttcttc                                                        >  1:1017171/1‑150 (MQ=255)
                                                                                           cGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCg                                                       <  2:679698/150‑1 (MQ=255)
                                                                                             aaTAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGt                                                                                                                            <  1:563247/79‑1 (MQ=255)
                                                                                             aaTAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGt                                                                                                                            >  2:563247/1‑79 (MQ=255)
                                                                                                  aCCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGtt                                                     >  1:736472/1‑145 (MQ=255)
                                                                                                  aCCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGtt                                                     <  2:736472/145‑1 (MQ=255)
                                                                                                    cGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGc                                              >  2:586952/1‑150 (MQ=255)
                                                                                                          ccGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGccc                                        <  1:586952/150‑1 (MQ=255)
                                                                                                                gggAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATcacc                                  >  1:776766/1‑150 (MQ=255)
                                                                                                                         tGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGc                         >  1:244304/1‑150 (MQ=255)
                                                                                                                              aaCTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCtt                    <  1:1101434/150‑1 (MQ=255)
                                                                                                                                     aaaaaaaTGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTAc                                                                                                                   >  1:275952/1‑48 (MQ=255)
                                                                                                                                     aaaaaaaTGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTAc                                                                                                                   <  2:275952/48‑1 (MQ=255)
                                                                                                                                       tcacaTGCTCGCCAACCCCGGCAAGTTTTGACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCAc           <  2:974026/146‑1 (MQ=255)
                                                                                                                                                cGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGTAGGTGAc  >  2:172465/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TTCGGCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGTAGGTGAC  >  NC_002947/5966240‑5966533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑