| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 418,298 | C→G | 13.9% | L26L (CTC→CTG) | PP_0345 → | heptose kinase WapP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 418,298 | 0 | C | G | 13.9% | 133.3 / 12.1 | 49 | L26L (CTC→CTG) | PP_0345 | heptose kinase WapP |
| Reads supporting (aligned to +/- strand): ref base C (19/23); new base G (2/5); total (21/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGCTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATG > NC_002947/418149‑418441 | gctgctCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTCCGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTc < 2:738404/150‑1 (MQ=255) tgctCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCa > 1:824803/1‑150 (MQ=255) ctggctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGaa < 1:118506/150‑1 (MQ=255) tggctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGAAc < 1:655730/150‑1 (MQ=255) ggctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGAAca < 1:273658/150‑2 (MQ=255) gctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGTGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGAAcac < 1:814573/150‑3 (MQ=255) ctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGc < 1:850825/150‑1 (MQ=255) tggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCt > 1:435033/1‑150 (MQ=255) tggAGCGCAAGGCCCAGAAACTCTACGATCGCAAAAGACACTATGGGGATGCACTATGATGGCGAGTTGGACAATGGCGCCGGGGTACGAACACTTGGCGGCGGACTTCGCAAGCATCGAGGCGGAGTTAGCCCTGCAGGGCCAAAGGcc > 2:207176/1‑149 (MQ=255) gAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGNACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTga < 1:767329/150‑1 (MQ=255) cccAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgaccc < 1:986745/150‑1 (MQ=255) ccAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgacccg > 2:700365/1‑150 (MQ=255) ctACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGGCCCGCGACCCGCTCAGTGaa < 2:968489/150‑1 (MQ=255) gATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTgg > 1:623945/1‑150 (MQ=255) aTCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGt < 2:544104/150‑1 (MQ=255) tCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTa < 1:1200777/150‑1 (MQ=255) tCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTa < 2:935560/150‑1 (MQ=255) cGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTAc < 1:456458/150‑1 (MQ=255) aaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGa > 2:749438/1‑150 (MQ=255) aaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGa > 2:298583/1‑150 (MQ=255) cGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGc < 1:298583/150‑1 (MQ=255) cGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGc < 2:959138/150‑1 (MQ=255) gggATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACgg < 2:824803/150‑1 (MQ=255) tctGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAac > 2:887193/1‑148 (MQ=255) tctGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAac < 1:887193/148‑1 (MQ=255) aGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAg < 1:700365/150‑1 (MQ=255) acTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTacac > 2:887702/1‑150 (MQ=255) cgcCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGCGCGTGCGGTGTGGAACTACTACGTCAAGCGCTACACCgggg > 2:96267/1‑150 (MQ=255) cGGGGTCCGAACCCGACGCGCCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCg < 2:633760/150‑1 (MQ=255) ggTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCggg > 2:137540/1‑143 (MQ=255) ggTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCggg < 1:137540/143‑1 (MQ=255) cGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTg > 1:55646/1‑88 (MQ=255) cGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTg < 2:55646/88‑1 (MQ=255) ccccTGGCGGCGGACTCCGGCAGCCTCTACGCGGTGTTCGCCCTCCAGTGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGGCAAGCGCTACACCGGGGCCGGCAAGCACATg < 1:207176/148‑1 (MQ=255) cTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAgg > 2:525404/1‑46 (MQ=255) cTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAgg < 1:525404/46‑1 (MQ=255) cTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGt > 2:201719/1‑96 (MQ=255) cTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGt < 1:201719/96‑1 (MQ=255) cTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATgcgc < 1:887702/150‑1 (MQ=255) gcggACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTAc > 2:784291/1‑150 (MQ=255) cTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTggg > 2:652277/1‑150 (MQ=255) cTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCggg < 2:505644/111‑1 (MQ=255) cTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCggg > 1:505644/1‑111 (MQ=255) tCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGAATGCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCa > 1:316975/1‑150 (MQ=255) ggTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGc > 1:787609/1‑150 (MQ=255) ccgtTCGCCCTGGAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGggcggc > 2:512154/3‑136 (MQ=255) ccgtTCGCCCTGGAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGggcggc < 1:512154/134‑1 (MQ=255) tgtTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCg > 2:298044/1‑150 (MQ=255) tCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAAt < 2:1179635/150‑1 (MQ=255) cGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATg < 1:652277/150‑1 (MQ=255) | GCTGCTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATG > NC_002947/418149‑418441 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |