| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,002,100 | G→A | 9.3% | P86L (CCC→CTC) | amaA ← | L‑pipecolate oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,002,100 | 0 | G | A | 9.3% | 141.8 / 5.5 | 54 | P86L (CCC→CTC) | amaA | L‑pipecolate oxidase |
| Reads supporting (aligned to +/- strand): ref base G (30/19); new base A (2/3); total (32/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01 | |||||||||||
TGCGCCATGTGCAGCGTGCCTTTGTGCTGGGCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGTGGATGGCCGCCGACAGGCCGGTAATACCGCCGCCGATCACA > NC_002947/6001953‑6002249 | tgCGCTATGTGGAGCGTGTCTTTGTGCTGGGCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCTAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCCCGACGACGTCgagg < 2:409571/150‑1 (MQ=255)tgCGCCATGTGCAGCGTGCCTTTGTGCTGGGCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCgggg < 1:277873/150‑1 (MQ=255) gcgcCATGTGCAGCGTGCCTTTGTGCTGGGCCTGGCAGTCGATGCCGAGGCGCTAGCTGAGTTCTAACACTTAAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCCCGACGACGTCGAGGc < 2:465958/150‑1 (MQ=255) gcCATGTGCAGCGTGCCTTTGTGCTGGGCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCgg > 2:763827/1‑150 (MQ=255) gcCATGTGCAGCGTGCCTTTGTGCTGGGCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCgg > 2:281570/1‑150 (MQ=255) ccATGTGCAGCGTGCCTTTGTGCTGGGCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGa < 2:971953/150‑1 (MQ=255) gtgCAGCGTGCCTTTGTGCTGGGCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCa < 2:1099192/150‑1 (MQ=255) cTTTGTGCTGGGCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGCCCACGGGTGGCCTCGACCTCGTCGGGGCGGAGCCTCGTGCCgtggt > 2:333071/1‑146 (MQ=255) cTGGGCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAg < 2:355418/150‑1 (MQ=255) tGGGCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAgg < 1:1067555/150‑1 (MQ=255) gggCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGc > 2:288459/1‑150 (MQ=255) ggCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCCCGACGACGTCGAGGcca < 2:977967/124‑3 (MQ=255) ggCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCCCGACGACGTCGAGGcca > 1:977967/1‑122 (MQ=255) gCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCg < 2:275616/150‑1 (MQ=255) cTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGAc > 2:898856/1‑150 (MQ=255) gCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGtt > 1:617771/1‑150 (MQ=255) aGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTgc > 2:612262/1‑150 (MQ=255) tCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTgcgc > 1:1067453/1‑150 (MQ=255) tGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCgg > 1:913410/1‑150 (MQ=255) cGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGc < 2:1090695/150‑1 (MQ=255) gcgcTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCAcc > 1:646491/1‑150 (MQ=255) cTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTg > 2:140944/1‑150 (MQ=255) aTGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCgg < 1:1072242/88‑1 (MQ=255) aTGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCgg > 2:1072242/1‑88 (MQ=255) aTGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATc < 1:618517/150‑1 (MQ=255) cGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTcc > 2:308435/1‑150 (MQ=255) cacTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGc < 1:763827/150‑1 (MQ=255) acTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCCCGACGACGTCGAGGccac > 2:796519/1‑82 (MQ=255) acTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCCCGACGACGTCGAGGccac < 1:796519/85‑4 (MQ=255) ttCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTc > 1:466762/1‑150 (MQ=255) ttCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTc > 2:56175/1‑150 (MQ=255) ttCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTc > 1:427911/1‑150 (MQ=255) ttCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGAACAGGCGGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTc > 1:560220/1‑150 (MQ=255) tCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCAGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGtt < 2:117289/98‑1 (MQ=255) tCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCAGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGtt > 1:117289/1‑98 (MQ=255) aGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAg > 1:460344/1‑150 (MQ=255) gCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGc > 1:392722/1‑103 (MQ=255) gCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGc < 2:392722/103‑1 (MQ=255) ggggCTTCGCCAAGCAGCTTGTTCAGGCGGCTACCCGGCTTCTGTCAAATGGGGGCCTCGACGTCGTTGGGGCGGATGCACGTGACGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCGACCGTGGCCGGTCTTCCGGGCCTCCAGCACg > 2:72897/1‑150 (MQ=255) ttCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCAc < 1:288459/150‑1 (MQ=255) cAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACt > 1:441105/1‑150 (MQ=255) ttGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGt < 2:441105/150‑1 (MQ=255) ggcTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGt < 2:460344/150‑1 (MQ=255) tACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGt < 2:344649/93‑1 (MQ=255) tACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGt > 1:344649/1‑93 (MQ=255) tACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGTGGa > 2:305238/1‑150 (MQ=255) aCCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGTGGAt < 1:305238/150‑1 (MQ=255) cccTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGTGGATg > 1:686755/1‑150 (MQ=255) gCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGTGGATGgccg > 1:1016340/1‑150 (MQ=255) ttCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGTGGATGgccgcc < 1:308435/150‑1 (MQ=255) cAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGTGGATGGCCGCCGACAGGc < 1:281570/150‑1 (MQ=255) tGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGt < 2:513176/126‑1 (MQ=255) tGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGt > 1:513176/1‑126 (MQ=255) tGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGTGGATGGCCGCCGACAGGCCGGTaa < 1:140944/150‑1 (MQ=255) cTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGTGGATGGCCGCCGACAGGCCGGTAATAcc > 1:670513/1‑150 (MQ=255) cTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTACGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGTGGATGGCCGCCGACAGGCCGGTAATAcc > 2:595057/1‑150 (MQ=255) gtcgtcGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGTGGATGGCCGCCGACAGGCCGGTAATAccgccgcc > 2:817895/1‑150 (MQ=255) tcgtcgGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGTGGATGGCCGCCGACAGGCCGGTAATAccgccgccg > 1:363708/1‑150 (MQ=255) gggCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGTGGATGGCCGCCGACAGGCCGGTAGTACCGCCGCCGATcaca < 2:617771/150‑1 (MQ=255) | TGCGCCATGTGCAGCGTGCCTTTGTGCTGGGCCTGGCAGTCGATGCCGAGGCGCTCGATCATGGCGAACACTTCAGCCGGGGCTTCGCCAAGCACCTTGTTCAGGCGGCTACCCTGCTTCTGGCCAAGGGTGGCCTCGACGTCGTCGGGGCGGATCCACGTGCCGGCGTTGACCAGGCCGACGTTGCGCCCGGAGCCACCGTGGCCGATCTTCCAGGCCTCCAGCACGATCACCGACTTGCCCTGTTCGAGCAGGTGGATGGCCGCCGACAGGCCGGTAATACCGCCGCCGATCACA > NC_002947/6001953‑6002249 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |