Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,005,043 T→G 9.6% intergenic (+25/‑151) PP_5259 → / → ydcJ LysR family transcriptional regulator/metalloprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,005,0430TG 9.6% 133.1 / 8.1 52intergenic (+25/‑151)PP_5259/ydcJLysR family transcriptional regulator/metalloprotein
Reads supporting (aligned to +/- strand):  ref base T (20/27);  new base G (2/3);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01

CCGTGGAACCATGCAATGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCA  >  NC_002947/6004899‑6005182
                                                                                                                                                |                                                                                                                                           
ccGTGGAACCATGCAATGCGCAGTGCCGGAGCGCATTACCTTGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTAc                                                                                                                                        <  2:484177/150‑1 (MQ=255)
  gTGGAACCATGCAATGCGCAGTGCCGGCGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACgg                                                                                                                                      <  1:1115478/150‑1 (MQ=255)
        ccATGCAATGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACcg                                                                                                                                >  1:63560/1‑150 (MQ=255)
          aTGCAATGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACcgcg                                                                                                                              >  1:997118/1‑150 (MQ=255)
            gCAATGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACggg                                                                                                                                     >  2:431137/1‑141 (MQ=255)
            gCAATGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACggg                                                                                                                                     <  1:431137/141‑1 (MQ=255)
             cAATGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCCTTTCCCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAg                                                                                                                           <  1:465847/150‑1 (MQ=37)
               aTGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGCGGTCCCCGTAgga                                                                                                                         <  1:1179116/150‑2 (MQ=255)
                 gcgcAGTGCCGGAGCGCATTACCTGGCGTGGGGCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGTTCCTACGGGGACCGCGTAGgcgc                                                                                                                       <  1:907741/150‑1 (MQ=255)
                 gcgcAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACggg                                                                                                                                     >  1:95569/1‑136 (MQ=255)
                 gcgcAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACggg                                                                                                                                     <  2:95569/136‑1 (MQ=255)
                 gcgcAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGgcgc                                                                                                                       <  2:614551/150‑1 (MQ=255)
                                 cATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCAGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGaaa                                                                                                       >  2:540251/1‑150 (MQ=255)
                                     aCCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGc                                                                                                   <  1:768883/150‑1 (MQ=255)
                                      ccTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCAGGGAAAGGGcc                                                                                                  <  1:88055/150‑1 (MQ=255)
                                        tGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCggg                                                                                                          >  1:112409/1‑140 (MQ=255)
                                        tGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCggg                                                                                                          <  2:112409/140‑1 (MQ=255)
                                        tGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGt                                                                                                >  1:263559/1‑150 (MQ=255)
                                                     gCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAAAGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCaa                                                                                   >  1:1150060/1‑150 (MQ=255)
                                                                      cAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACAcact                                                                  >  1:909621/1‑150 (MQ=255)
                                                                       aaaGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGACTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGaca                                                                      >  2:544777/1‑145 (MQ=255)
                                                                       aaaGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGACTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGaca                                                                      <  1:544777/145‑1 (MQ=255)
                                                                                ggCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACttttttt                                                        <  2:63560/150‑1 (MQ=255)
                                                                                ggCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGGAGCgg                                                                                                                     <  2:918054/89‑1 (MQ=21)
                                                                                ggCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGGAGCgg                                                                                                                     >  1:918054/1‑89 (MQ=21)
                                                                                 gcgcTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTc                                                       >  2:446553/1‑150 (MQ=255)
                                                                                   gcTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGc                                                     <  2:437718/150‑1 (MQ=255)
                                                                                        tggAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTACGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTtgtg                                                <  1:446553/150‑1 (MQ=255)
                                                                                         ggAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGa                                               <  2:1150060/150‑1 (MQ=255)
                                                                                           aaTGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACttttt                                                          <  1:751617/137‑1 (MQ=255)
                                                                                           aaTGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACttttt                                                          >  2:751617/1‑137 (MQ=255)
                                                                                              ggATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCaaa                                          <  2:263559/150‑1 (MQ=255)
                                                                                                      gagaAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCTCTTTTGAGCAAAGTGCATTc                                  >  2:444168/1‑150 (MQ=255)
                                                                                                        gCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGacaca                                                                    <  2:263515/114‑1 (MQ=255)
                                                                                                        gCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGacaca                                                                    >  1:263515/1‑114 (MQ=255)
                                                                                                         cAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCaaaaaa                                                                               >  1:586643/1‑102 (MQ=255)
                                                                                                         cAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCaaaaaa                                                                               <  2:586643/102‑1 (MQ=255)
                                                                                                           gTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCttt                                                                                                                 >  2:894035/1‑66 (MQ=255)
                                                                                                           gTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCttt                                                                                                                 <  1:894035/66‑1 (MQ=255)
                                                                                                            ttGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGa                                                                        >  1:52365/1‑106 (MQ=255)
                                                                                                            ttGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGa                                                                        <  2:52365/106‑1 (MQ=255)
                                                                                                            ttGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCt                            >  1:770707/1‑150 (MQ=255)
                                                                                                                  gTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGacac                                                                     <  2:70427/103‑1 (MQ=255)
                                                                                                                  gTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGacac                                                                     >  1:70427/1‑103 (MQ=255)
                                                                                                                   tttGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGt                     <  2:997118/150‑1 (MQ=255)
                                                                                                                     tGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTaa                   <  2:770707/150‑1 (MQ=255)
                                                                                                                         ggCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGt               >  2:511785/1‑150 (MQ=255)
                                                                                                                           ccTCTTCGCGGCTAAAGCCGATCCTACGGAGACCGCGTAGGTGTGGCTTTAGCAGGGAAAGGGCCGTTGAGAACAAAAAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCTCAGTTTTCAGGTAAGCGTcc             <  2:356453/150‑1 (MQ=255)
                                                                                                                                  ccgGCTAAAGCCGCGCCTACGCGGTCCCCGTAGGAGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTtgt                                                 >  2:629899/2‑107 (MQ=21)
                                                                                                                                  ccgGCTAAAGCCGCGCCTACGCGGTCCCCGTAGGAGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTtgt                                                 <  1:629899/106‑1 (MQ=21)
                                                                                                                                    ggCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAAc    <  1:540251/150‑1 (MQ=255)
                                                                                                                                      cTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCa  <  2:42980/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                           
CCGTGGAACCATGCAATGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCA  >  NC_002947/6004899‑6005182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑