Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,030,405 T→A 8.8% intergenic (+34/+33) PP_5729 → / ← rpmG hypothetical protein/50S ribosomal protein L33

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,030,4050TA 8.8% 134.2 / 4.6 46intergenic (+34/+33)PP_5729/rpmGhypothetical protein/50S ribosomal protein L33
Reads supporting (aligned to +/- strand):  ref base T (17/24);  new base A (2/2);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

CGGCCGCACCCGGGTGGTCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTGTCGGTGGTGTAGAA  >  NC_002947/6030257‑6030548
                                                                                                                                                     |                                                                                                                                               
cGGCCGCACCCGGGTGGTCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTc                                                                                                                                                <  1:226796/150‑1 (MQ=255)
   ccGCACCCGGGTGGTCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGa                                                                                                                                             >  1:162967/1‑150 (MQ=255)
      cACCCGGGTGGTCGGCCCCAAAGAAATGTGCACCCTCTAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAACACCCGCCTCCTTTCGGATGc                                                                                                                                          <  1:311497/150‑1 (MQ=255)
       aCCCGGGTGGTCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCg                                                                                                                                         <  2:1053217/150‑1 (MQ=255)
         ccGAGTGGACGGCCCCAACGAAACGCGCACCCGCGAGTGCGAGGTCGAGCGTAACCAGAACAATGCCGGTGCCGACCTGCTGATCCAGGGCTTCACCTTCAACTGCCACAGAAAAACAA‑AAAAAAAAACCCGCCTCCTTTCGGATGCggg                                                                                                                                       <  2:676731/150‑1 (MQ=255)
           gggTGGTCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGtt                                                                                                                                     <  2:877662/150‑1 (MQ=255)
                 tCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTc                                                                                                                               >  1:1122234/1‑150 (MQ=255)
                  cGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTCCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCa                                                                                                                              <  2:402410/150‑1 (MQ=255)
                   ggCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAg                                                                                                                             >  1:1133045/1‑150 (MQ=255)
                    gCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAgg                                                                                                                            <  2:1133045/150‑1 (MQ=255)
                             aaaTGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCATCCGAAAGGAGGCGGGTTTTTTTCAGGTCGCCGATc                                                                                                                   <  2:1144242/150‑1 (MQ=39)
                                tgtgCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAAt                                                                                                                <  1:923968/150‑1 (MQ=255)
                                     aCCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACtt                                                                                                           <  2:1109458/150‑1 (MQ=255)
                                     aCCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACtt                                                                                                           >  2:1051209/1‑150 (MQ=255)
                                          cgAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCt                                                                                                      <  2:1097311/150‑1 (MQ=255)
                                                        aGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTac                                                                                        <  1:344064/150‑1 (MQ=255)
                                                              tCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCATCCGAAAGGAGGCGGGtttttttt                                                                                                                               <  1:1194815/105‑2 (MQ=25)
                                                              tCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCATCCGAAAGGAGGCGGGtttttttt                                                                                                                               >  2:1194815/1‑104 (MQ=25)
                                                                     cAAGGCCGCTGCCGCCCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTACAACAGCAAAAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTg                                                                            <  2:1030009/150‑1 (MQ=255)
                                                                        ggCCGGTGCCGACCTGCTGATCCAGGGCCTCGACTCCAACTGACCCTGCGACGGCG‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGaa                                                                        >  1:1056653/1‑150 (MQ=255)
                                                                           cGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAACCCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGaacaa                                                                     <  1:1051209/150‑1 (MQ=255)
                                                                                 cGACATACTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑ACAAAATGTACAACTTGCCCGTGGAGGGTGTTCCTTTTCAGTGCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGAt                                                               <  1:274558/150‑1 (MQ=255)
                                                                                           acccAGGGCGTCAACTTCAACTGACCCTGCAACAGCT‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTTGTTGATCCCTTACTTCATCATTGCTTACTTATTCACCACGTTCGTTCTAACTACCGGAGCATATGTCtt                                                     <  2:12488/148‑1 (MQ=255)
                                                                                                   tttCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGa                                             <  2:1061712/149‑1 (MQ=255)
                                                                                                    ttCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTAc                                                                                                             >  2:137889/1‑85 (MQ=255)
                                                                                                    ttCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTAc                                                                                                             <  1:137889/85‑1 (MQ=255)
                                                                                                        aCTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTg                                        >  1:305128/1‑150 (MQ=255)
                                                                                                          ttCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCtt                                                                                            >  1:778838/1‑96 (MQ=255)
                                                                                                          ttCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCtt                                                                                            <  2:778838/96‑1 (MQ=255)
                                                                                                             aaCTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCgg                                   >  1:1019412/1‑150 (MQ=255)
                                                                                                                 gACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGggtg                               <  2:162967/150‑1 (MQ=255)
                                                                                                                    ccTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGgtggtg                            >  1:1062915/1‑150 (MQ=255)
                                                                                                                      tGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTgcg                          >  2:1061387/1‑150 (MQ=255)
                                                                                                                               c‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCtt                 <  2:1019412/150‑1 (MQ=255)
                                                                                                                                 tgaaaaaaaCCCGCATCCGAAAGGAGGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTACGGGGGGGTGCGCTTGTTCttt                >  2:160811/3‑149 (MQ=38)
                                                                                                                                 aaaaaaaaaCCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCCATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGGTCATATTTCTTGATCTCGATTTTGTCCGGCGTGGTGCGCTTGTTCTTg                >  1:657214/1‑150 (MQ=255)
                                                                                                                                 aaaaaaaaaCCCGCCTCCTTTCGGAAGCGGGGTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTg                >  1:1125468/1‑150 (MQ=255)
                                                                                                                                     aaaaaCCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTGTCgg            >  1:332594/1‑150 (MQ=255)
                                                                                                                                      aaaaCCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTGTCggt           <  2:1122234/150‑1 (MQ=255)
                                                                                                                                         aCCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTGTCggtggt        <  2:1062915/150‑1 (MQ=255)
                                                                                                                                           ccGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGggtg                               >  1:1150321/1‑125 (MQ=255)
                                                                                                                                           ccGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGggtg                               <  2:1150321/125‑1 (MQ=255)
                                                                                                                                           ccGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTAATCTCGTTTTTCTCCGGGGTGGTGCGCTTGTTCTTGTCGGTGgtgt      >  1:1172291/1‑150 (MQ=255)
                                                                                                                                               ctcctTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCatat                                                          <  2:375018/94‑1 (MQ=255)
                                                                                                                                               ctcctTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCatat                                                          >  1:375018/1‑94 (MQ=255)
                                                                                                                                               ctcctTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTGTCGGTGGTGTAGaa  <  2:305128/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
CGGCCGCACCCGGGTGGTCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTGTCGGTGGTGTAGAA  >  NC_002947/6030257‑6030548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑