Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,030,410 T→G 10.8% intergenic (+39/+28) PP_5729 → / ← rpmG hypothetical protein/50S ribosomal protein L33

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,030,4100TG10.8% 111.2 / 3.0 45intergenic (+39/+28)PP_5729/rpmGhypothetical protein/50S ribosomal protein L33
Reads supporting (aligned to +/- strand):  ref base T (15/24);  new base G (2/3);  total (18/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCCGGGTGGTCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTGTCGGTGGTGTAGAAGTGGCCGG  >  NC_002947/6030263‑6030556
                                                                                                                                                    |                                                                                                                                                  
cACCCGGGTGGTCGGCCCCAAAGAAATGTGCACCCTCTAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAACACCCGCCTCCTTTCGGATGc                                                                                                                                                  <  1:311497/150‑1 (MQ=255)
 aCCCGGGTGGTCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCg                                                                                                                                                 <  2:1053217/150‑1 (MQ=255)
   ccGAGTGGACGGCCCCAACGAAACGCGCACCCGCGAGTGCGAGGTCGAGCGTAACCAGAACAATGCCGGTGCCGACCTGCTGATCCAGGGCTTCACCTTCAACTGCCACAGAAAAACAA‑AAAAAAAAACCCGCCTCCTTTCGGATGCggg                                                                                                                                               <  2:676731/150‑1 (MQ=255)
     gggTGGTCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGtt                                                                                                                                             <  2:877662/150‑1 (MQ=255)
           tCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTc                                                                                                                                       >  1:1122234/1‑150 (MQ=255)
            cGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTCCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCa                                                                                                                                      <  2:402410/150‑1 (MQ=255)
             ggCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAg                                                                                                                                     >  1:1133045/1‑150 (MQ=255)
              gCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAgg                                                                                                                                    <  2:1133045/150‑1 (MQ=255)
                       aaaTGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCATCCGAAAGGAGGCGGGTTTTTTTCAGGTCGCCGATc                                                                                                                           <  2:1144242/150‑1 (MQ=39)
                          tgtgCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAAt                                                                                                                        <  1:923968/150‑1 (MQ=255)
                               aCCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACtt                                                                                                                   >  2:1051209/1‑150 (MQ=255)
                               aCCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACtt                                                                                                                   <  2:1109458/150‑1 (MQ=255)
                                    cgAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCt                                                                                                              <  2:1097311/150‑1 (MQ=255)
                                                  aGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTac                                                                                                <  1:344064/150‑1 (MQ=255)
                                                        tCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCATCCGAAAGGAGGCGGGtttttttt                                                                                                                                       >  2:1194815/1‑104 (MQ=25)
                                                        tCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCATCCGAAAGGAGGCGGGtttttttt                                                                                                                                       <  1:1194815/105‑2 (MQ=25)
                                                               cAAGGCCGCTGCCGCCCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTACAACAGCAAAAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTg                                                                                    <  2:1030009/150‑1 (MQ=255)
                                                                  ggCCGGTGCCGACCTGCTGATCCAGGGCCTCGACTCCAACTGACCCTGCGACGGCG‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGaa                                                                                >  1:1056653/1‑150 (MQ=255)
                                                                     cGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAACCCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGaacaa                                                                             <  1:1051209/150‑1 (MQ=255)
                                                                           cGACATACTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑ACAAAATGTACAACTTGCCCGTGGAGGGTGTTCCTTTTCAGTGCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGAt                                                                       <  1:274558/150‑1 (MQ=255)
                                                                                     acccAGGGCGTCAACTTCAACTGACCCTGCAACAGCT‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTTGTTGATCCCTTACTTCATCATTGCTTACTTATTCACCACGTTCGTTCTAACTACCGGAGCATATGTCtt                                                             <  2:12488/148‑1 (MQ=255)
                                                                                             tttCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGa                                                     <  2:1061712/149‑1 (MQ=255)
                                                                                              ttCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTAc                                                                                                                     <  1:137889/85‑1 (MQ=255)
                                                                                              ttCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTAc                                                                                                                     >  2:137889/1‑85 (MQ=255)
                                                                                                  aCTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTg                                                >  1:305128/1‑150 (MQ=255)
                                                                                                    ttCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCtt                                                                                                    >  1:778838/1‑96 (MQ=255)
                                                                                                    ttCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCtt                                                                                                    <  2:778838/96‑1 (MQ=255)
                                                                                                       aaCTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCgg                                           >  1:1019412/1‑150 (MQ=255)
                                                                                                           gACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGggtg                                       <  2:162967/150‑1 (MQ=255)
                                                                                                              ccTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGgtggtg                                    >  1:1062915/1‑150 (MQ=255)
                                                                                                                tGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTgcg                                  >  2:1061387/1‑150 (MQ=255)
                                                                                                                         c‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCtt                         <  2:1019412/150‑1 (MQ=255)
                                                                                                                           tgaaaaaaaCCCGCATCCGAAAGGAGGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTACGGGGGGGTGCGCTTGTTCttt                        >  2:160811/3‑149 (MQ=38)
                                                                                                                           aaaaaaaaaCCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCCATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGGTCATATTTCTTGATCTCGATTTTGTCCGGCGTGGTGCGCTTGTTCTTg                        >  1:657214/1‑150 (MQ=255)
                                                                                                                           aaaaaaaaaCCCGCCTCCTTTCGGAAGCGGGGTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTg                        >  1:1125468/1‑150 (MQ=255)
                                                                                                                               aaaaaCCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTGTCgg                    >  1:332594/1‑150 (MQ=255)
                                                                                                                                aaaaCCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTGTCggt                   <  2:1122234/150‑1 (MQ=255)
                                                                                                                                   aCCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTGTCggtggt                <  2:1062915/150‑1 (MQ=255)
                                                                                                                                     ccGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGggtg                                       <  2:1150321/125‑1 (MQ=255)
                                                                                                                                     ccGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGggtg                                       >  1:1150321/1‑125 (MQ=255)
                                                                                                                                     ccGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTAATCTCGTTTTTCTCCGGGGTGGTGCGCTTGTTCTTGTCGGTGgtgt              >  1:1172291/1‑150 (MQ=255)
                                                                                                                                         ctcctTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCatat                                                                  <  2:375018/94‑1 (MQ=255)
                                                                                                                                         ctcctTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCatat                                                                  >  1:375018/1‑94 (MQ=255)
                                                                                                                                         ctcctTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTGTCGGTGGTGTAGaa          <  2:305128/150‑1 (MQ=255)
                                                                                                                                                 ggATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTGTCGGTGGTGTAGAAGTGGCCgg  <  2:1056653/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CACCCGGGTGGTCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCC‑AAAAAAAAACCCGCCTCCTTTCGGATGCGGGTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGGTGGTGCGCTTGTTCTTGTCGGTGGTGTAGAAGTGGCCGG  >  NC_002947/6030263‑6030556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑