| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,043,722 | T→A | 9.7% | intergenic (+18/+16) | gmk → / ← PP_5297 | deoxyguanylate kinase/guanylate kinase/amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,043,722 | 0 | T | A | 9.7% | 184.8 / 9.4 | 62 | intergenic (+18/+16) | gmk/PP_5297 | deoxyguanylate kinase/guanylate kinase/amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base T (23/33); new base A (3/3); total (26/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GACTACGTCATCATCAATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACGAACACCAGC > NC_002947/6043573‑6043870 | gtcgaaatcatcattgATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCAt > 1:441994/8‑150 (MQ=255) tACGTCATCATCAATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGc < 1:1138192/150‑1 (MQ=255) gTCATCATCAATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCTGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGc < 1:718088/150‑1 (MQ=255) tcatcattgATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGcc < 2:441994/150‑1 (MQ=255) catcatcaATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGccc > 2:1177576/1‑150 (MQ=255) catcaATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCaa < 1:1192333/150‑1 (MQ=255) catcaATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCAATGCAGCCCCat < 2:1178981/150‑2 (MQ=255) tcaATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGCAGAAGCAGCAGCAGCGTCATGCGGCATTGCTAAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATg > 2:899474/1‑150 (MQ=255) tgatgaTTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGttt > 1:389080/1‑150 (MQ=255) gatgatTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTa < 1:720590/150‑1 (MQ=255) cacGGCGCTGGCAGACTTGAAATCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCg < 1:1088926/150‑1 (MQ=255) cTGGCAGACTTGAAACCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGt < 1:2841/150‑1 (MQ=255) gCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGGGGCATTTCTCAAGCGCATGATCGGCTGACGACCGATGGGGCTGCATCGCTGCTCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGc < 1:1177576/150‑1 (MQ=255) gCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGtt > 1:725611/1‑127 (MQ=255) gCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGtt < 2:725611/127‑1 (MQ=255) cTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCctgct < 1:47516/150‑1 (MQ=255) ttGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATg < 2:364145/118‑1 (MQ=255) ttGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATg > 1:364145/1‑118 (MQ=255) ttGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCt > 2:584026/1‑128 (MQ=255) ttGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCt < 1:584026/128‑1 (MQ=255) ttGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCctgctg > 2:329835/1‑150 (MQ=255) gAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGAc < 2:389080/150‑1 (MQ=255) aGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCt > 1:1136494/1‑123 (MQ=255) aGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCt < 2:1136494/123‑1 (MQ=255) aGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCa > 2:1010494/1‑150 (MQ=255) gCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAg < 2:1038674/150‑1 (MQ=255) aaTCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGaa < 1:1010494/150‑1 (MQ=255) ggCTGCTGCTTCAGCAGGAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAAAGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTAc < 1:439048/150‑1 (MQ=255) tgctgAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCAgc > 1:445671/1‑150 (MQ=255) aagaagCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGcgcacg > 2:927844/1‑150 (MQ=255) gaagCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGcgcacgca > 1:997641/1‑150 (MQ=255) gcagcagcagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATgc > 2:519878/1‑150 (MQ=255) cagcagcagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGCCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATgcg < 2:849522/150‑1 (MQ=255) gcagaagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATACGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGcgcg > 1:85688/1‑150 (MQ=255) gcagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACa < 2:476259/150‑1 (MQ=255) cagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGc < 2:219702/114‑1 (MQ=255) cagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGc > 1:219702/1‑114 (MQ=255) cagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATAGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAg < 1:987700/150‑1 (MQ=255) gCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAg < 2:929529/150‑1 (MQ=255) ggCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGc < 1:572937/150‑1 (MQ=255) ttGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCTGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGAt < 1:927844/150‑1 (MQ=255) tCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTg < 1:489539/40‑1 (MQ=255) tCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTg > 2:489539/1‑40 (MQ=255) gCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAg > 1:80172/1‑85 (MQ=255) gCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAg < 2:80172/85‑1 (MQ=255) aaaTGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGcatcat > 2:569126/1‑131 (MQ=255) aaaTGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGcatcat < 1:569126/131‑1 (MQ=255) aaTGATCGGCTGAAGATCGATGGGGCTGCATTGAAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCg > 1:1187843/1‑150 (MQ=255) gCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGc > 2:890500/1‑127 (MQ=255) gCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGc < 1:890500/127‑1 (MQ=255) cattGGGGCTGCAATGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAg > 1:823088/4‑107 (MQ=255) cattGGGGCTGCAATGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAg < 2:823088/104‑1 (MQ=255) cattGGGGCTGCAATGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATc > 2:251001/4‑150 (MQ=255) cGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATc < 2:445671/150‑1 (MQ=255) attGGGGCTGCAATGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGcatcat > 2:1080093/3‑112 (MQ=255) attGGGGCTGCAATGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGcatcat < 1:1080093/110‑1 (MQ=255) aTGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCAc < 1:329835/150‑1 (MQ=255) gggCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGc > 2:289787/1‑81 (MQ=255) gggCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGc < 1:289787/81‑1 (MQ=255) ggCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGt < 2:1061881/76‑1 (MQ=255) ggCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGt > 1:1061881/1‑76 (MQ=255) cTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACGAacac < 1:519878/150‑1 (MQ=255) aTTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACGAACAccagc > 1:632208/1‑150 (MQ=255) | GACTACGTCATCATCAATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACGAACACCAGC > NC_002947/6043573‑6043870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |