Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,048,351 C→G 12.7% P257R (CCT→CGT)  PP_5300 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,048,3510CG12.7% 149.3 / 13.9 55P257R (CCT→CGT) PP_5300LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (23/25);  new base G (4/3);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01

CAACACACCTCCGGAGCACCTGGTTGGCCGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATGCGCTGCCCGGGGTGGA  >  NC_002947/6048205‑6048498
                                                                                                                                                  |                                                                                                                                                   
cAACACACCTCCGGAGCACCTGGTTGGCCGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTaa                                                                                                                                                  >  1:697558/1‑149 (MQ=255)
    acacCTCCGGAGCACCTGGTTGGCCGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGGGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACggtg                                                                                                                                              >  2:809475/1‑150 (MQ=255)
    acacCTCCGGAGCACCTGGTTGGCCGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACggtg                                                                                                                                              >  1:1105238/1‑150 (MQ=255)
    acacCTCCGGAGCACCTGGTTGGCCGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACggtg                                                                                                                                              <  2:754844/150‑1 (MQ=255)
       ccgcctgAGCACCGGTGTGGCCGCAACCGGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTc                                                                                                                                           >  2:16464/7‑150 (MQ=255)
           cGGAGCACCTGGTTGGCCGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGgc                                                                                                                                       <  1:94494/150‑1 (MQ=255)
           cGGAGCACCTGGTTGGCCGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGgc                                                                                                                                       <  2:1022962/150‑1 (MQ=255)
             gAGCACCTGGTTGGCCGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCGTAGGGTGGTcctgcat                                                                                                                                     <  1:538131/150‑7 (MQ=255)
                cACCTGGTTGGCCGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCTGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGcaac                                                                                                                                  >  2:182981/1‑150 (MQ=255)
                cACCTGGTTGGCCGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGcaac                                                                                                                                  >  1:983110/1‑150 (MQ=255)
                 aCCTGGTTGGCCGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGcaacc                                                                                                                                 <  1:705552/150‑1 (MQ=255)
                   cTGGTTGGCCGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGcaaccaa                                                                                                                               >  2:340517/1‑150 (MQ=255)
                    tGGTTGGCCGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCTGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGcaaccaac                                                                                                                              <  1:182981/150‑1 (MQ=255)
                           ccGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACAccc                                                                                                                       <  1:340517/150‑1 (MQ=255)
                           ccGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGCCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGCATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCACCAACcccc                                                                                                                       >  1:610479/1‑150 (MQ=255)
                           ccGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCACCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGAACCACCCTACGGTGGTCTGGCGCAACCAAAAacactc                                                                                                                       >  2:183047/1‑148 (MQ=255)
                                 ccTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCt                                                                                                                 <  1:464831/150‑1 (MQ=255)
                                              tcctATGTAGTCTGCGGCCAGCCACAGTGGCGCTAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTAc                                                                                                    <  2:697558/150‑1 (MQ=255)
                                              tcctATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTAc                                                                                                    <  2:1105238/150‑1 (MQ=255)
                                              tcctATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTAc                                                                                                    <  2:98107/150‑1 (MQ=255)
                                               cctATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGcaaccaa                                                                                                                               <  2:364058/122‑1 (MQ=255)
                                               cctATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGcaaccaa                                                                                                                               >  1:364058/1‑122 (MQ=255)
                                                ctATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCa                                                                                                  >  1:978039/1‑150 (MQ=255)
                                                ctATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCa                                                                                                  <  2:983110/150‑1 (MQ=255)
                                                     tAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTgca                                                                                             <  2:978039/150‑1 (MQ=255)
                                                                    cacaGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTAc                                                                                                                                                  <  1:568802/82‑1 (MQ=255)
                                                                    cacaGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTAc                                                                                                                                                  >  2:568802/1‑82 (MQ=255)
                                                                       aggggCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCg                                                                           <  1:16464/147‑1 (MQ=255)
                                                                               gaccgcCCCAGCTAGCGCCCCGCCAGCGTGCCGTGGATCGCACCAGACGGCTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTg                                                                   <  2:610479/147‑1 (MQ=255)
                                                                               gAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTg                                                                   <  1:809475/150‑1 (MQ=255)
                                                                                    ccTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAAt                                                              >  1:1192845/1‑150 (MQ=255)
                                                                                     cTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATg                                                             >  1:794420/1‑150 (MQ=255)
                                                                                      tCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTgca                                                                                             <  1:687747/117‑1 (MQ=255)
                                                                                      tCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTgca                                                                                             >  2:687747/1‑117 (MQ=255)
                                                                                        aGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAAAACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGTATGTCGACCATCGCCCAGTTGGTAATggcc                                                          <  1:183047/150‑1 (MQ=255)
                                                                                         gCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGt                                                                                    <  1:783785/123‑1 (MQ=255)
                                                                                         gCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGt                                                                                    >  2:783785/1‑123 (MQ=255)
                                                                                                 cccGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGc                                                 >  2:391325/1‑150 (MQ=255)
                                                                                                   cGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGt                                               <  2:652915/150‑1 (MQ=255)
                                                                                                                            gacgacTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTg                      <  1:432637/150‑1 (MQ=255)
                                                                                                                                  tCGCTGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTAGCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATgcg                >  2:866678/1‑150 (MQ=255)
                                                                                                                                       gccagACCACCGTAGGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATgcgc               >  1:305217/5‑146 (MQ=255)
                                                                                                                                       gccagACCACCGTAGGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATgcgc               <  2:305217/142‑1 (MQ=255)
                                                                                                                                       gccagACCACCGTAGGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATGCGCTGcc           >  1:619868/5‑150 (MQ=255)
                                                                                                                                        ccagACCACCGTAGGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATGCGCTGccc          >  1:1186412/4‑150 (MQ=255)
                                                                                                                                        ccagACCACCGTAGGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATGCGCTGccc          >  2:201273/4‑150 (MQ=255)
                                                                                                                                        ccagACCACCCTACGTTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTgg                                             >  1:1131677/4‑115 (MQ=255)
                                                                                                                                        ccagACCACCCTACGTTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTgg                                             <  2:1131677/112‑1 (MQ=255)
                                                                                                                                           gACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCCTGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATGCGCTGCCCggg       >  2:41133/1‑150 (MQ=255)
                                                                                                                                             ccaccGTAGGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATGCGCTGCCCGGGGt     <  2:619868/150‑1 (MQ=255)
                                                                                                                                             ccaccCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATg                  <  2:1175205/137‑1 (MQ=255)
                                                                                                                                             ccaccCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATg                  >  1:1175205/1‑137 (MQ=255)
                                                                                                                                             ccaccCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATGCGCTGCCCGGGGt     <  2:1192845/150‑1 (MQ=255)
                                                                                                                                              caccCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATGCGCTGCCCGGGGTg    >  2:584813/1‑150 (MQ=255)
                                                                                                                                              caccCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATGCGCTGCCCGGGGTg    >  2:309673/1‑150 (MQ=255)
                                                                                                                                                cccTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATGCGCTGCCCGGGGTGGa  >  1:867830/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CAACACACCTCCGGAGCACCTGGTTGGCCGCAACCTGGGTTCGACCTCCTATGTAGTCTGCGGCCAGCCACAGTGGCGCGAGCGCCTCAGCGAGTCCCCCGCCAGCGTGCCGTGGATCGCACCAGACGACTCGATGCAGGACCACCCTACGGTGGTCTGGCGCAACCAACAACACCCGGGCCTGAGCCCGCGCTACCAGTGCAGCAGCATGTCGACCATCGCCCAGTTGGTAATGGCCGGCCTGGGCGTGGCAGCACTGCCGGACTACATGGTGCATGCGCTGCCCGGGGTGGA  >  NC_002947/6048205‑6048498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑