Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,051,724 C→A 8.8% intergenic (+17/‑40) ridA → / → PP_5304 aminoacrylate/iminopropionate hydrolase/deaminase/lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,051,7240CA 8.8% 173.2 / 2.9 57intergenic (+17/‑40)ridA/PP_5304aminoacrylate/iminopropionate hydrolase/deaminase/lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (23/28);  new base A (2/3);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CCACTTCGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAG  >  NC_002947/6051575‑6051863
                                                                                                                                                     |                                                                                                                                           
ccACTTCGCACAGGTAAACGCGGTGCTAGGACCATGCCTTGAACAGCGCTATCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGcc                                                                                                                                             <  2:1037582/150‑1 (MQ=255)
  aCTTCGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCt                                                                                                                                           >  2:878243/1‑150 (MQ=255)
    ttCGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGc                                                                                                                                         >  1:1031808/1‑150 (MQ=255)
    ttCGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGc                                                                                                                                         >  1:1032249/1‑150 (MQ=255)
      cGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGccc                                                                                                                                       <  1:339903/150‑1 (MQ=255)
        ccAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGt                                                                                                                                     >  2:1160371/1‑150 (MQ=255)
        ccAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGt                                                                                                                                     <  1:247297/150‑1 (MQ=255)
            ggTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCAcc                                                                                                                                 <  1:1160371/150‑1 (MQ=255)
             gTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCAGGGCCCGTCAcca                                                                                                                                <  1:823111/150‑2 (MQ=255)
               aaaCGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGTACGCCATCCTGGTCATCGAATGATGCCCGGGTGACGGGCAGGGCCCGTCAccagg                                                                                                                              <  2:102198/150‑4 (MQ=255)
                         aTGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGc                                                                                                                    <  2:562983/150‑1 (MQ=255)
                           gggCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCaa                                                                                                                  <  1:744281/150‑1 (MQ=255)
                            ggCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAg                                                                                                                 <  1:167283/150‑1 (MQ=255)
                                gCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCAGGGCCCGTCAcca                                                                                                                                >  1:966904/1‑130 (MQ=255)
                                gCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCAGGGCCCGTCAcca                                                                                                                                <  2:966904/131‑2 (MQ=255)
                                     ttCGAACAGCCCTACCCGGCCCTCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCg                                                                                                        >  2:891901/1‑150 (MQ=255)
                                      tCGAACAGCACTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGACTGATGCCCTGGTGACGGCCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGt                                                                                                       <  1:788477/150‑1 (MQ=255)
                                         aaCAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCAt                                                                                                    >  2:512144/1‑150 (MQ=255)
                                         aaCAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCAt                                                                                                    <  1:878243/150‑1 (MQ=255)
                                                cTACCCGGCCCTCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCaa                                                                                             <  1:891901/150‑1 (MQ=255)
                                                        cccGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGcc                                                                                     >  1:391796/1‑150 (MQ=255)
                                                            cgccgcCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTc                                                                                 <  1:1030335/150‑1 (MQ=255)
                                                             gccgccATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGc                                                                                                  <  2:953461/132‑1 (MQ=255)
                                                             gccgccATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGc                                                                                                  >  1:953461/1‑132 (MQ=255)
                                                              ccgccATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTcgc                                                                               <  2:1032249/150‑1 (MQ=255)
                                                              ccgccATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTcgc                                                                               <  2:1031808/150‑1 (MQ=255)
                                                                   aTCGGCGTTGCCGCACTGACGAAACGCGCCCAGGTCGAAATGGCCGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCGTTGTGGATCAACCCGCTGCTGCGGCAATTTCACACCGTCATGCGTCAAGCACTGACCTCCGCGCTgg                                                                          >  1:444687/1‑150 (MQ=255)
                                                                            gCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGc                                                                 >  1:673246/1‑150 (MQ=255)
                                                                             ccGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCt                                                                >  2:809493/1‑150 (MQ=255)
                                                                              cGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTa                                                               >  1:1038719/1‑150 (MQ=255)
                                                                               gCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGATACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTAc                                                              >  1:379101/1‑150 (MQ=255)
                                                                                          aGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGc                                                   <  2:927902/150‑1 (MQ=255)
                                                                                                       gAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAAc                                      <  1:1085810/150‑1 (MQ=255)
                                                                                                        aaaTGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCt                                                                            <  2:982957/111‑1 (MQ=255)
                                                                                                        aaaTGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCt                                                                            >  1:982957/1‑111 (MQ=255)
                                                                                                        aaaTGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACCGCCCTTCGCGCTGGCAGCCCTGCTACTGggcggc                                                      <  2:877746/133‑1 (MQ=255)
                                                                                                        aaaTGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACCGCCCTTCGCGCTGGCAGCCCTGCTACTGggcggc                                                      >  1:877746/1‑133 (MQ=255)
                                                                                                             gACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAgg                                                                                                                >  1:653931/1‑70 (MQ=255)
                                                                                                             gACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAgg                                                                                                                <  2:653931/70‑1 (MQ=255)
                                                                                                             gACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTAc                                                              <  1:654291/120‑1 (MQ=255)
                                                                                                             gACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTAc                                                              >  2:654291/1‑120 (MQ=255)
                                                                                                               cGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGAt                              <  2:673246/150‑1 (MQ=255)
                                                                                                                gCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGAtt                             <  2:391796/150‑1 (MQ=255)
                                                                                                                gCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGATGCAc                                                 >  1:1195454/1‑130 (MQ=255)
                                                                                                                gCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGATGCAc                                                 <  2:1195454/130‑1 (MQ=255)
                                                                                                                      cTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACg                       >  1:753031/1‑150 (MQ=255)
                                                                                                                          tCATCGAATGATGCCCTGGTGCCGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAAc                        <  1:305573/145‑1 (MQ=255)
                                                                                                                          tCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAAc                        >  2:305573/1‑145 (MQ=255)
                                                                                                                            aTCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTTCACCAGCCACAAACCCGTAGATTTCAACGGCATCt                 <  1:41217/150‑1 (MQ=255)
                                                                                                                             tCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTg                <  1:809493/150‑1 (MQ=255)
                                                                                                                                      gCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCa       >  2:717640/1‑150 (MQ=255)
                                                                                                                                          tGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGaa   <  2:1178226/150‑1 (MQ=255)
                                                                                                                                          tGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGaa   >  1:427053/1‑150 (MQ=255)
                                                                                                                                           ggTGACGGGCAGGGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAg  >  2:531541/1‑150 (MQ=255)
                                                                                                                                             tGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGggcgg                                                       >  2:1015220/1‑95 (MQ=255)
                                                                                                                                             tGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGggcgg                                                       <  1:1015220/95‑1 (MQ=255)
                                                                                                                                                 gggCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATc            <  2:281303/134‑1 (MQ=255)
                                                                                                                                                 gggCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATc            >  1:281303/1‑134 (MQ=255)
                                                                                                                                                     |                                                                                                                                           
CCACTTCGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAG  >  NC_002947/6051575‑6051863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑