Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,081,045 T→A 20.9% intergenic (+36/+26) PP_5332 → / ← PP_5333 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,081,0450TA20.9% 109.7 / 30.0 59intergenic (+36/+26)PP_5332/PP_5333hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (25/21);  new base A (5/7);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.64e-01

CAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGGTGTAGAT  >  NC_002947/6080902‑6081193
                                                                                                                                               |                                                                                                                                                    
cAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCcgc                                                                                                                                                <  2:582421/150‑1 (MQ=255)
   cGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                             <  1:1019618/150‑1 (MQ=255)
     aaTGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGAaga                                                                                                                                           <  2:716650/150‑1 (MQ=255)
           gtgCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCgg                                                                                                                                     <  1:1128020/150‑1 (MQ=255)
                 cGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAAt                                                                                                                               <  2:601619/150‑1 (MQ=255)
                    gaCAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCGTGGAGAGAGTGATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCAGGATTGCACGCGATGAGGACGGGGCAAtttt                                                                                                                            >  2:582320/1‑148 (MQ=255)
                     ccAGCGAATGTGTCGGCTGACCTGGTTCAGGGTAAGCGTGGGGCGAGTCATTCGCTGGGCGTCCAGTTCACGGGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATc                                                                                                                           <  1:327012/149‑1 (MQ=255)
                       aGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCaa                                                                                                                         >  2:459665/1‑150 (MQ=255)
                        gCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCaaa                                                                                                                        <  2:1065062/150‑1 (MQ=255)
                         cGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGAGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAg                                                                                                                       <  1:64830/150‑1 (MQ=255)
                          gAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                             >  1:374108/1‑127 (MQ=255)
                          gAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                             <  2:374108/127‑1 (MQ=255)
                                     aTGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTc                                                                                                           <  2:962657/150‑1 (MQ=255)
                                            ggtggAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCAGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCCTCCaccacc                                                                                                    >  2:1089185/1‑150 (MQ=255)
                                             gtggAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGAAGAGGCCgg                                                                                                                                     >  1:272220/1‑116 (MQ=255)
                                             gtggAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGAAGAGGCCgg                                                                                                                                     <  2:272220/116‑1 (MQ=255)
                                                   ttGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTAGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCt                                                                                             >  2:254998/1‑150 (MQ=255)
                                                   ttGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGACTTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCt                                                                                             >  1:157792/1‑150 (MQ=255)
                                                     gAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAg                                                                                                                       >  1:562214/1‑122 (MQ=255)
                                                     gAAGCCTGGGGCCAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAg                                                                                                                       <  2:562214/122‑1 (MQ=255)
                                                       aGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTc                                                                                         <  1:261247/150‑1 (MQ=255)
                                                           tGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGTGCTTGCCCGCGCAGAGGCAGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCttcc                                                                                     >  2:345732/1‑147 (MQ=255)
                                                           tGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCa                                                                                     <  1:459665/150‑1 (MQ=255)
                                                                    tCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCa                                                                            >  1:1077883/1‑150 (MQ=255)
                                                                    tCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCa                                                                            >  1:631898/1‑150 (MQ=255)
                                                                       ttCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAg                                                                         >  1:1185433/1‑150 (MQ=255)
                                                                       ttCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGAGGGCTTGCCCGCGAAGAGGCCGGGGGAATTATATGAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAg                                                                         >  1:378668/1‑150 (MQ=255)
                                                                               gCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGAaga                                                                                                                                           <  1:939946/76‑1 (MQ=37)
                                                                               gCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGAaga                                                                                                                                           >  2:939946/1‑76 (MQ=37)
                                                                                 gTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCcgcg                                                                                                                                               >  2:669118/1‑70 (MQ=38)
                                                                                 gTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCcgcg                                                                                                                                               <  1:669118/70‑1 (MQ=255)
                                                                                   ccAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCAGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCCTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGcc                                                             <  1:1089185/150‑1 (MQ=255)
                                                                                     aGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAg                                                           >  2:941182/1‑150 (MQ=255)
                                                                                     aGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAg                                                           >  1:1186167/1‑150 (MQ=255)
                                                                                      gATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAgg                                                          >  2:636416/1‑150 (MQ=255)
                                                                                           gTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCAtt                                                     <  2:1185433/150‑1 (MQ=255)
                                                                                              gtgAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTg                                                    <  2:942709/148‑1 (MQ=255)
                                                                                              gtgAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTg                                                    >  1:942709/1‑148 (MQ=255)
                                                                                               tgAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTg                                                 >  2:4807/1‑150 (MQ=255)
                                                                                                  aaaTATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGAcc                                              <  2:509664/150‑1 (MQ=255)
                                                                                                   aaTATCTGAGCCCTGCTC‑TTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCCAAGGTCTGCGTCTTCCACCACCCTCCCCTTGTCTGCATTCCGCGCATAGGCCGCCTCCGCCCGGTTCTTGCTTACCtt                                            >  2:6050/1‑150 (MQ=255)
                                                                                                          gaccccTCCTC‑TTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCAcc                                     <  1:6050/147‑1 (MQ=255)
                                                                                                                tGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAg                                >  1:442434/1‑150 (MQ=255)
                                                                                                                   tctTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCtt                             <  2:442854/150‑1 (MQ=255)
                                                                                                                     tttttttACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTcc                                        >  1:650103/1‑137 (MQ=255)
                                                                                                                     tttttttACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTcc                                        <  2:650103/137‑1 (MQ=255)
                                                                                                                              cGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCAt                                                                                    >  2:882455/1‑84 (MQ=255)
                                                                                                                              cGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCAt                                                                                    <  1:882455/84‑1 (MQ=255)
                                                                                                                                gCCTCTTCGCGGGCATACCCCACAGGATGCTGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCcac                >  2:1012207/1‑150 (MQ=255)
                                                                                                                                   tctTCGCGGGCAAGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCa                                                                                 <  1:973699/82‑1 (MQ=255)
                                                                                                                                   tctTCGCGGGCAAGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCa                                                                                 >  2:973699/1‑82 (MQ=255)
                                                                                                                                    ctTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCg            >  1:791971/1‑150 (MQ=255)
                                                                                                                                     ttCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCAcc                                     <  1:117564/124‑1 (MQ=255)
                                                                                                                                     ttCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCAcc                                     >  2:117564/1‑124 (MQ=255)
                                                                                                                                      tCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCggg          <  1:4807/150‑1 (MQ=255)
                                                                                                                                      tCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGAATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCggg          >  1:495296/1‑150 (MQ=255)
                                                                                                                                            gCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAgg                                                                        >  2:826365/1‑82 (MQ=255)
                                                                                                                                            gCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAgg                                                                        <  1:826365/82‑1 (MQ=255)
                                                                                                                                            gCTCGCCCGCGAAGAGGCCGGGGCAATTATCAACGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCTAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGGTGTAg    <  2:885061/150‑1 (MQ=255)
                                                                                                                                              ttGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGGTGTAGat  <  2:1186167/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGGTGTAGAT  >  NC_002947/6080902‑6081193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑