Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 541,443 C→T 7.0% P1273L (CCG→CTG)  rpoB → DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947541,4430CT 7.0% 167.2 / 3.1 57P1273L (CCG→CTG) rpoBDNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (28/25);  new base T (2/2);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01

CCAGATGGTGCTGTTCGATGGCCGTACCGGCAACAAGTTCGAGCGTCCTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAA  >  NC_002947/541294‑541587
                                                                                                                                                     |                                                                                                                                                
ccAGATGGTGCTGTTCGATGGCCGTACCGGCAACAAGTTCGAGCGTCCTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGcc                                                                                                                                                  >  1:451168/1‑150 (MQ=255)
       gTGATGTTCGATGGCCGTACCGGCAACAAGTTCGAGCGTCCTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGggt                                                                                                                                           <  2:386463/150‑1 (MQ=255)
         gCTGTTCGATGGCCGTACCGGCAACAAGTTCGAGCGTCCTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTgg                                                                                                                                             >  1:999813/1‑146 (MQ=255)
         gCTGTTCGATGGCCGTACCGGCAACAAGTTCGAGCGTCCTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTgg                                                                                                                                             <  2:999813/146‑1 (MQ=255)
             ttCGATGGCCGTACCGGCAACAAGTTCGAGCGTCCTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAg                                                                                                                                     <  1:391447/150‑1 (MQ=255)
                  tGGCCGTACCGGCAACAAGTTCGAGCGTCCTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCa                                                                                                                                >  1:905770/1‑150 (MQ=255)
                     ccGTACCGGCAACAAGTTCGAGCGTCCTGTGACCGTTGGTTACATCTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGtt                                                                                                                             <  1:860774/150‑1 (MQ=255)
                      cGTACCGGCAACAAGTTCGAGCGTCCTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTc                                                                                                                            >  1:208091/1‑150 (MQ=255)
                           cGGCAACAAGTTCGAGCGTCCTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCggtgg                                                                                                                       >  2:629162/1‑150 (MQ=255)
                                  aaGTTCGAGCGTCCTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGt                                                                                                                >  2:946717/1‑150 (MQ=255)
                                        gAGCGTCCTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCggg                                                                                                          <  1:863547/150‑1 (MQ=255)
                                          gCGTCCTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGggtgacca                                                                                                                                      >  1:1185120/1‑116 (MQ=255)
                                          gCGTCCTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGggtgacca                                                                                                                                      <  2:1185120/120‑5 (MQ=255)
                                              ccTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATg                                                                                                    <  1:629162/150‑1 (MQ=255)
                                               cTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGGTGTCAGCGTTTCGGGGAGATgg                                                                                                   <  1:766068/150‑1 (MQ=255)
                                                 gtgACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGaa                                                                                                 <  2:981787/150‑1 (MQ=255)
                                                 gtgACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGaa                                                                                                 <  2:905770/150‑1 (MQ=255)
                                                     ccGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAgtgt                                                                                             >  2:961439/1‑150 (MQ=255)
                                                       gTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTgg                                                                                           <  2:208091/150‑1 (MQ=255)
                                                       gTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTgg                                                                                           <  1:961439/150‑1 (MQ=255)
                                                           gTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGGGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGgcgc                                                                                       >  1:739947/1‑150 (MQ=255)
                                                                            aaGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAg                                                                                 >  1:894622/1‑139 (MQ=255)
                                                                            aaGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAg                                                                                 <  2:894622/139‑1 (MQ=255)
                                                                              gCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTGCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTACGGCGAGTTGGATGTGTGGGCGCTGGAAGCATACGGCGCGGc                                                                    >  2:698940/1‑150 (MQ=255)
                                                                                 gAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATa                                                                 <  1:698940/150‑1 (MQ=255)
                                                                                     cACTTGGTGGAAGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACAcc                                                             <  2:1004350/150‑1 (MQ=255)
                                                                                                cgacAAGATGCACGCGCGTTCCACTGGTTCCTACAGACTGGTTACCCAGCAGCCGCTGGGTGGTAAGGGGCAGTCAGCTGGTCAGCGTTTAGGGGAGACGGAGGCGTGGGCGCTTGAAGGATACGGCGCGGCATACACCCTGCAAGAAAt                                                  >  1:682131/1‑150 (MQ=255)
                                                                                                 gacAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGaa                                                                                                 >  2:738007/1‑102 (MQ=255)
                                                                                                 gacAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGaa                                                                                                 <  1:738007/102‑1 (MQ=255)
                                                                                                   cAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCggg                                                                                                          >  1:57302/1‑91 (MQ=255)
                                                                                                   cAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCggg                                                                                                          <  2:57302/91‑1 (MQ=255)
                                                                                                   cAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCt                                               >  1:319396/1‑150 (MQ=255)
                                                                                                      gATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTg                                                                                                                                              >  1:925945/1‑52 (MQ=255)
                                                                                                      gATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTg                                                                                                                                              <  2:925945/52‑1 (MQ=255)
                                                                                                        tGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAg                                          >  2:720463/1‑150 (MQ=255)
                                                                                                           aCGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACAcc                                                             >  1:463762/1‑128 (MQ=255)
                                                                                                           aCGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACAcc                                                             <  2:463762/128‑1 (MQ=255)
                                                                                                               gcgTTCCACTGGTTCCTACAGCCAGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGACGCTGGAAGCATACCGCGCGGCATACACCCTGCACGAAATGCTCACAGTGAACTc                                   >  2:223869/1‑150 (MQ=255)
                                                                                                                  ttCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGga                                >  2:1080174/1‑150 (MQ=255)
                                                                                                                     cACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGaaa                                                   >  1:1027519/1‑128 (MQ=255)
                                                                                                                     cACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGaaa                                                   <  2:1027519/128‑1 (MQ=255)
                                                                                                                     cACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGgacga                             >  1:414823/1‑150 (MQ=255)
                                                                                                                      aCTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGCAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGgacgac                            <  2:451168/150‑1 (MQ=255)
                                                                                                                          gTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATg                                                 <  1:147885/125‑1 (MQ=255)
                                                                                                                          gTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATg                                                 >  2:147885/1‑125 (MQ=255)
                                                                                                                            tCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGATGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAAc                      <  1:675435/150‑1 (MQ=255)
                                                                                                                                  cgCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGt                >  2:829010/2‑150 (MQ=255)
                                                                                                                                  aGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCggtg                                                                                                                        <  2:600245/46‑1 (MQ=255)
                                                                                                                                  aGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCggtg                                                                                                                        >  1:600245/1‑46 (MQ=255)
                                                                                                                                        gTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCaa           <  1:1058115/149‑1 (MQ=255)
                                                                                                                                        gTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCaa           >  2:1058115/1‑149 (MQ=255)
                                                                                                                                          caCCCAGCGGCTGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAgtgt                                                                                             <  2:270299/64‑1 (MQ=255)
                                                                                                                                          caCCCAGCGGCTGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAgtgt                                                                                             >  1:270299/2‑65 (MQ=39)
                                                                                                                                            cccAGCGGCTGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGgcgc                                                                       <  2:860170/85‑1 (MQ=255)
                                                                                                                                            cccAGCGGCTGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGgcgc                                                                       >  1:860170/1‑85 (MQ=255)
                                                                                                                                              cagcagCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTAc    >  2:262638/1‑150 (MQ=255)
                                                                                                                                               agcagcCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACa   >  1:1125811/1‑150 (MQ=255)
                                                                                                                                                gcagcCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGAGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATggtcga  >  1:179549/1‑145 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CCAGATGGTGCTGTTCGATGGCCGTACCGGCAACAAGTTCGAGCGTCCTGTGACCGTTGGTTACATGTACATGCTCAAGCTGAACCACTTGGTGGACGACAAGATGCACGCGCGTTCCACTGGTTCCTACAGCCTGGTTACCCAGCAGCCGCTGGGTGGTAAGGCGCAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAA  >  NC_002947/541294‑541587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑