Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 550,552 C→G 8.5% intergenic (+30/‑112) tufB → / → rpsJ elongation factor Tu‑B/30S ribosomal protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947550,5520CG 8.5% 174.7 / 8.2 59intergenic (+30/‑112)tufB/rpsJelongation factor Tu‑B/30S ribosomal protein S10
Reads supporting (aligned to +/- strand):  ref base C (28/26);  new base G (2/3);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.04e-01

AGATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTT  >  NC_002947/550410‑550700
                                                                                                                                              |                                                                                                                                                    
aGATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCgggg                                                                                                                                               >  1:1013090/1‑150 (MQ=255)
 gATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCggggg                                                                                                                                              <  2:1013090/150‑1 (MQ=255)
  aTGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTGAGCGGGGGt                                                                                                                                             <  2:420383/150‑1 (MQ=255)
   ggACTGTTATCCTGATCAATTCCATCGCAATGGAAGACGGGCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTGAGCGGGGGtt                                                                                                                                            <  2:192352/149‑1 (MQ=25)
     aCTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGtttt                                                                                                                                          >  2:1074331/1‑150 (MQ=255)
       tGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGtttttt                                                                                                                                        >  1:1031526/1‑150 (MQ=255)
            cccTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTAtt                                                                                                                                   >  1:993812/1‑150 (MQ=255)
               tGATAAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTggg                                                                                                                                <  2:460103/150‑1 (MQ=255)
                  tCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATGGGGTTg                                                                                                                             >  1:566801/1‑150 (MQ=255)
                  tCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTGTTTTATTGGGTTg                                                                                                                             >  2:681294/1‑150 (MQ=255)
                     aGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGTTTTTTTTTATTGGGTTGaca                                                                                                                          >  2:402974/1‑150 (MQ=255)
                     aGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGCCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGaca                                                                                                                          >  1:626808/1‑150 (MQ=255)
                      gACCATCGCAATCGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGacac                                                                                                                         <  1:1166556/150‑1 (MQ=255)
                                     aGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCgg                                                                                                                                                 >  1:414091/1‑111 (MQ=255)
                                     aGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCgg                                                                                                                                                 <  2:414091/111‑1 (MQ=255)
                                      gACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCta                                                                                                         >  2:656296/1‑150 (MQ=255)
                                           tCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGTCGGCGTTTTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATTGaa                                                                                                    <  1:948178/150‑1 (MQ=255)
                                           tCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGaa                                                                                                    >  1:532021/1‑150 (MQ=255)
                                               cgcTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCAc                                                                                                <  2:532021/150‑1 (MQ=255)
                                               cgcTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGCTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCAc                                                                                                <  1:260486/150‑1 (MQ=255)
                                                  ttCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACg                                                                                               <  2:157822/148‑1 (MQ=255)
                                                  ttCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACg                                                                                               >  1:157822/1‑148 (MQ=255)
                                                  ttCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGcc                                                                                             >  2:783508/1‑150 (MQ=255)
                                                   tCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGcct                                                                                            <  2:626808/150‑1 (MQ=255)
                                                      cTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCTTCTATAGAATCACGcctcct                                                                                         >  2:124655/1‑150 (MQ=255)
                                                       tATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTACtt                                                                                        >  1:124035/1‑150 (MQ=255)
                                                       tATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAAACCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGGCTATAGAATCACGCCTCCtt                                                                                        >  2:229190/1‑150 (MQ=255)
                                                        aTCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCttt                                                                                       <  2:993812/150‑1 (MQ=255)
                                                           cGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAg                                                                                    >  2:735552/1‑150 (MQ=255)
                                                            gTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGc                                                                                   <  2:1031526/150‑1 (MQ=255)
                                                             tGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCg                                                                                  >  1:119710/1‑150 (MQ=255)
                                                              gAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCgg                                                                                 <  1:783508/150‑1 (MQ=255)
                                                                 ggCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCg                                                                              >  2:575476/1‑150 (MQ=255)
                                                                    ggTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAg                                                                           <  1:575476/150‑1 (MQ=255)
                                                                     gTCGTACCGGCGGCCCCCGCCGTGTGGTCAAAATCTTCGAATAATCACTCGACCCATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGt                                                                          <  2:240617/150‑1 (MQ=255)
                                                                              tCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGtt                                                                 <  1:656296/150‑1 (MQ=255)
                                                                                               gCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTc                                                <  1:1074331/150‑1 (MQ=255)
                                                                                                ccAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTgggg                                                                                                                >  2:961745/1‑85 (MQ=255)
                                                                                                ccAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTgggg                                                                                                                <  1:961745/85‑1 (MQ=255)
                                                                                                    aaTCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGaaa                                           >  1:663700/1‑150 (MQ=255)
                                                                                                       catcGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTg                                                                <  2:440981/126‑1 (MQ=255)
                                                                                                       catcGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTg                                                                >  1:440981/1‑126 (MQ=255)
                                                                                                                aTCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTggg                                                                                                                                <  2:883591/53‑1 (MQ=255)
                                                                                                                aTCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTggg                                                                                                                                >  1:883591/1‑53 (MQ=255)
                                                                                                                aTCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTg                                                                                                                   <  1:745603/66‑1 (MQ=255)
                                                                                                                aTCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTg                                                                                                                   >  2:745603/1‑66 (MQ=255)
                                                                                                                aTCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCaaaa                               <  1:1153821/150‑1 (MQ=255)
                                                                                                                   aCTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGTTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCa                            <  1:402974/150‑1 (MQ=255)
                                                                                                                        aCCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGaaa                                           <  2:183705/130‑1 (MQ=255)
                                                                                                                        aCCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGaaa                                           >  1:183705/1‑130 (MQ=255)
                                                                                                                                aaaaaaCCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATc               >  1:890184/1‑150 (MQ=255)
                                                                                                                                 aaaaaCCCCCGCTGAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGACGTAGTGCGTCCGTTGGGAACAGCCTAGAGTCTGAAATCCAATGCAAAATCAGCAAATccatgtcg              >  1:16225/1‑144 (MQ=255)
                                                                                                                                 aaaaaCCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCa              <  2:890184/150‑1 (MQ=255)
                                                                                                                                  aaaaCCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTg                                                     >  1:485518/1‑110 (MQ=255)
                                                                                                                                  aaaaCCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTg                                                     <  2:485518/110‑1 (MQ=255)
                                                                                                                                  aaaaCCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAg             <  2:119710/150‑1 (MQ=255)
                                                                                                                                     aCCCCCGCTGAGCGGGGGTTTTTTTTTTTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGaa                                                            >  2:780745/1‑100 (MQ=255)
                                                                                                                                     aCCCCCGCTGAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGaa                                                            <  1:780745/100‑1 (MQ=255)
                                                                                                                                             tCAGCGGGGGTATTTTTTTTTAGGGTGAAAATGATTGTGGGCGGCTGTTTGATGAGTGCTTCTTTTAGCGGGCGTAGTGCGTCCGTTGGGACCACACTGGAAACATAAATCCAATGCAAAATTAGCAAATCCTTATTAGGTTGAAGGCtt  >  2:264373/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
AGATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTT  >  NC_002947/550410‑550700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑